A 1H NMR Method for the Analysis of Antigen-Antibody Interactions: Binding of a Peptide Fragment of Lysozyme to Anti-Lysozyme Monoclonal Antibody

1987 ◽  
Vol 102 (3) ◽  
pp. 643-649 ◽  
Author(s):  
Wataru ITO ◽  
Miwa NISHIMURA ◽  
Nobuo SAKATO ◽  
Hajime FUJIO ◽  
Yoji ARATA
Keyword(s):  
Monoclonal Antibody ◽  
1H Nmr ◽  
Peptide Fragment ◽  
Nmr Method ◽  
Antigen Antibody ◽  
Antibody Interactions

scholarly journals Molecular Docking and Molecular Dynamics (MD) Simulation of Human Anti Complement Factor H (CFH) Antibody Ab42 and CFH Polypeptide (pCFH)

2018 ◽  
Author(s):  
Bing Yang ◽  
Jiayi Ren ◽  
Tong Liu ◽  
Shujian Lin ◽  
Yueming Wang ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Molecular Docking ◽  
Md Simulation ◽  
Factor H ◽  
Energy Calculation ◽  
Complement Factor ◽  
Alanine Scanning ◽  
Computational Alanine Scanning ◽  
Antigen Antibody ◽  
Antibody Interactions

The details of antigen-antibody interactions and the identification of epitopes are critical for the development of monoclonal antibody drugs. Ab42 is a native human-derived anti-CFH monoclonal antibody. In this study, the interaction between antigen pCFH and antibody (Ab42) was theoretically demonstrated by molecular docking and MD simulation, combined with free energy calculation and computational alanine scanning (CAS), and key amino acids and epitopes were identified. Experimental alanine scanning (EAS) was then carried out to verify the results of the calculation, and our results indicated that Ab42 antibody forms hydrogen bonds and interacts hydrophobically with pCFH through the Tyr315, Ser100, Gly33, and Tyr53 residues on its CDR, while the main pCFH epitopes are located at the six sites of Pro441, Ile442, Asp443, Asn444, Ile447, and Thr448. In conclusion, this study has explored the mechanism of antigen-antibody interaction from both theoretical and experimental aspects, and our results have important theoretical significance for the design and development of relevant antibody drugs.

Two-Dimensional1H-nmr study of antigen-antibody interactions: Binding of synthetic decapeptides to an anti-acetylcholine receptor monoclonal antibody

Biopolymers ◽  
1991 ◽  
Vol 31 (6) ◽  
pp. 769-776 ◽  
Author(s):  
Manh Thong Cung ◽  
Pascal Demange ◽  
Michel Marraud ◽  
Vassilios Tsikaris ◽  
Constantinos Sakarellos ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Acetylcholine Receptor ◽  
Nmr Study ◽  
Antigen Antibody ◽  
Antibody Interactions ◽  
Receptor Monoclonal Antibody ◽  
Anti Acetylcholine

Radioimmunodetection of Malignant Melanoma with the 225.28S Monoclonal Antibody to HMW-MAA

1986 ◽  
Vol 25 (06) ◽  
pp. 220-224 ◽  
Author(s):  
G. L. Buraggi
Keyword(s):  
Monoclonal Antibody ◽  
Pilot Study ◽  
Malignant Melanoma ◽  
Prospective Study ◽  
Clinical Application ◽  
Multicenter Trial ◽  
Clinical Applications ◽  
Gamma Energy ◽  
A Prospective Study ◽  
Antigen Antibody

A review of the studies on the use of the antigen-antibody system HMW-MAA 225.28S in melanoma radioimmunodetection is reported. The results obtained in a pilot study (42 patients with 74 lesions), a multicenter trial (254 patients with 553 lesions) and a prospective study still outstanding (29 patients with 38 lesions) allow to consider this system as suitable for clinical application. F(ab′)2 labelled with 99mTc gave the best results in terms of positivity. Moreover this radioisotope allows the best dosimetric conditions. The gamma energy emitted by this radionuclide is particularly convenient for conventional scintillation cameras and ECT. Very good results in terms of sensitivity (70%-85%) and especially specificity (about 100%) were achieved. Possible clinical applications of the method are discussed.

Polyhydroxyalkanoate quantification in organic wastes and pure cultures using a single-step extraction and 1H NMR analysis

2012 ◽  
Vol 66 (5) ◽  
pp. 1000-1006 ◽  
Author(s):  
Elisabeth Linton ◽  
Asif Rahman ◽  
Sridhar Viamajala ◽  
Ronald C. Sims ◽  
Charles D. Miller
Keyword(s):  
1H Nmr ◽  
Azotobacter Vinelandii ◽  
Nile Blue ◽  
Agricultural Waste ◽  
Accurate Method ◽  
Single Step ◽  
Proton Nuclear Magnetic Resonance ◽  
Copolymer Composition ◽  
Nmr Analysis ◽  
Nmr Method

In this study, a proton nuclear magnetic resonance (1H NMR) method was developed to quantitatively analyze polyhydroxyalkanoate (PHA) content in Cupriavidus necator H16, Azotobacter vinelandii AvOP, and mixed microbial cultures from the effluent of an agricultural waste treatment anaerobic digester. In contrast to previous methods, a single-step PHA extractive method using deuterated chloroform was established, thereby facilitating direct 1H NMR analysis. The accuracy of the method was verified through comparison with well-established gas chromatography (GC) methanolysis techniques. Nile blue fluorescence staining was also carried out to serve as an independent and qualitative indicator of intracellular PHA content. The results indicate that the 1H NMR method is appropriate for rapid and non-destructive quantification of overall PHA content and determination of PHA copolymer composition in a variety of cultures. Notably, this technique was effective in measuring PHA content in full-strength waste samples where high concentrations of background impurities and organic compounds are present. The straightforward procedures minimize error-introducing steps, require less time and materials, and result in an accurate method suitable for routine analyses.

ChemInform Abstract: A 1H NMR Method for the Determination of Enantiomeric Excess and Absolute Configuration of cis-Dihydrodiol Metabolites of Polycyclic Arenes and Heteroarenes.

ChemInform ◽  
2010 ◽  
Vol 23 (40) ◽  
pp. no-no
Author(s):  
D. R. BOYD ◽  
N. D. SHARMA ◽  
R. BOYLE ◽  
R. A. S. MCMORDIE ◽  
J. CHIMA ◽  
...  
Keyword(s):  
1H Nmr ◽  
Absolute Configuration ◽  
Enantiomeric Excess ◽  
Nmr Method
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