Preparation of the Fv fragment from a short-chain mouse IgG2a anti-dansyl monoclonal antibody and use of selectively deuterated Fv analogs for two-dimensional proton NMR analyses of the antigen-antibody interactions

Biochemistry ◽  
1991 ◽  
Vol 30 (11) ◽  
pp. 2840-2847 ◽  
Author(s):  
Hideo Takahashi ◽  
Takako Igarashi ◽  
Ichio Shimada ◽  
Yoji Arata
Keyword(s):  
Monoclonal Antibody ◽  
Proton Nmr ◽  
Short Chain ◽  
Two Dimensional ◽  
Antigen Antibody ◽  
Antibody Interactions ◽  
Mouse Igg2a

scholarly journals Molecular Docking and Molecular Dynamics (MD) Simulation of Human Anti Complement Factor H (CFH) Antibody Ab42 and CFH Polypeptide (pCFH)

2018 ◽  
Author(s):  
Bing Yang ◽  
Jiayi Ren ◽  
Tong Liu ◽  
Shujian Lin ◽  
Yueming Wang ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Molecular Docking ◽  
Md Simulation ◽  
Factor H ◽  
Energy Calculation ◽  
Complement Factor ◽  
Alanine Scanning ◽  
Computational Alanine Scanning ◽  
Antigen Antibody ◽  
Antibody Interactions

The details of antigen-antibody interactions and the identification of epitopes are critical for the development of monoclonal antibody drugs. Ab42 is a native human-derived anti-CFH monoclonal antibody. In this study, the interaction between antigen pCFH and antibody (Ab42) was theoretically demonstrated by molecular docking and MD simulation, combined with free energy calculation and computational alanine scanning (CAS), and key amino acids and epitopes were identified. Experimental alanine scanning (EAS) was then carried out to verify the results of the calculation, and our results indicated that Ab42 antibody forms hydrogen bonds and interacts hydrophobically with pCFH through the Tyr315, Ser100, Gly33, and Tyr53 residues on its CDR, while the main pCFH epitopes are located at the six sites of Pro441, Ile442, Asp443, Asn444, Ile447, and Thr448. In conclusion, this study has explored the mechanism of antigen-antibody interaction from both theoretical and experimental aspects, and our results have important theoretical significance for the design and development of relevant antibody drugs.

Two-Dimensional1H-nmr study of antigen-antibody interactions: Binding of synthetic decapeptides to an anti-acetylcholine receptor monoclonal antibody

Biopolymers ◽  
1991 ◽  
Vol 31 (6) ◽  
pp. 769-776 ◽  
Author(s):  
Manh Thong Cung ◽  
Pascal Demange ◽  
Michel Marraud ◽  
Vassilios Tsikaris ◽  
Constantinos Sakarellos ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Acetylcholine Receptor ◽  
Nmr Study ◽  
Antigen Antibody ◽  
Antibody Interactions ◽  
Receptor Monoclonal Antibody ◽  
Anti Acetylcholine

Radioimmunodetection of Malignant Melanoma with the 225.28S Monoclonal Antibody to HMW-MAA

1986 ◽  
Vol 25 (06) ◽  
pp. 220-224 ◽  
Author(s):  
G. L. Buraggi
Keyword(s):  
Monoclonal Antibody ◽  
Pilot Study ◽  
Malignant Melanoma ◽  
Prospective Study ◽  
Clinical Application ◽  
Multicenter Trial ◽  
Clinical Applications ◽  
Gamma Energy ◽  
A Prospective Study ◽  
Antigen Antibody

A review of the studies on the use of the antigen-antibody system HMW-MAA 225.28S in melanoma radioimmunodetection is reported. The results obtained in a pilot study (42 patients with 74 lesions), a multicenter trial (254 patients with 553 lesions) and a prospective study still outstanding (29 patients with 38 lesions) allow to consider this system as suitable for clinical application. F(ab′)2 labelled with 99mTc gave the best results in terms of positivity. Moreover this radioisotope allows the best dosimetric conditions. The gamma energy emitted by this radionuclide is particularly convenient for conventional scintillation cameras and ECT. Very good results in terms of sensitivity (70%-85%) and especially specificity (about 100%) were achieved. Possible clinical applications of the method are discussed.

scholarly journals Peak-tracking algorithm for use in comprehensive two-dimensional liquid chromatography – Application to monoclonal-antibody peptides

2021 ◽  
Vol 1639 ◽  
pp. 461922
Author(s):  
Stef R.A. Molenaar ◽  
Tina A. Dahlseid ◽  
Gabriel M. Leme ◽  
Dwight R. Stoll ◽  
Peter J. Schoenmakers ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Liquid Chromatography ◽  
Tracking Algorithm ◽  
Two Dimensional ◽  
Peak Tracking

A two-dimensional NMR method for assignment of deoxyribose proton NMR signals in d(ApGpCpT)

1984 ◽  
Vol 121 (3) ◽  
pp. 1021-1026 ◽  
Author(s):  
Kofen Lai ◽  
Dinesh O. Shah ◽  
Eugene DeRose ◽  
David G. Gorenstein
Keyword(s):  
Proton Nmr ◽  
Two Dimensional ◽  
Nmr Method ◽  
Nmr Signals
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