scholarly journals Molecular phylogeny and genome evolution in the Drosophila virilis species group: duplications of the alcohol dehydrogenase gene

1996 ◽  
Vol 13 (1) ◽  
pp. 132-149 ◽  
Author(s):  
D. I. Nurminsky ◽  
E. N. Moriyama ◽  
E. R. Lozovskaya ◽  
D. L. Hartl
Keyword(s):  
Molecular Phylogeny ◽  
Alcohol Dehydrogenase ◽  
Genome Evolution ◽  
Species Group ◽  
Drosophila Virilis ◽  
Dehydrogenase Gene

scholarly journals The Alcohol Dehydrogenase Gene Is Nested in the outspread Locus of Drosophila melanogaster

Genetics ◽  
1996 ◽  
Vol 143 (2) ◽  
pp. 897-911 ◽  
Author(s):  
S McNabb ◽  
S Greig ◽  
T Davis
Keyword(s):  
Drosophila Melanogaster ◽  
Alcohol Dehydrogenase ◽  
Pcr Amplification ◽  
Transcription Unit ◽  
Adjacent Gene ◽  
Dehydrogenase Gene ◽  
Chromosomal Breakpoints ◽  
Splicing Patterns ◽  
Somatic Musculature

Abstract This report describes the structure and expression of the outspread (osp) gene of Drosophila melanogaster. Previous work showed that chromosomal breakpoints associated with mutations of the osp locus map to both sides of the alcohol dehydrogenase gene (Adh), suggesting that Adh and the adjacent gene Adh' are nested in osp. We extended a chromosomal walk and mapped additional osp mutations to define the maximum molecular limit of osp as 119 kb. We identified a 6-kb transcript that hybridizes to osp region DNA and is altered or absent in osp mutants. Accumulation of this RNA peaks during embryonic and pupal periods. The osp cDNAs comprise two distinct classes based on alternative splicing patterns. The 5′ end of the longest cDNA was extended by PCR amplification. When hybridized to the osp walk, the 5′ extension verifies that Adh and Adh' are nested in osp and shows that osp has a transcription unit of ≥74 kb. In situ hybridization shows that osp is expressed both maternally and zygotically. In the ovary, osp is transcribed in nurse cells and localized in the oocyte. In embryos, expression is most abundant in the developing visceral and somatic musculature.

scholarly journals The upstream stimulatory factor binds to and activates the promoter of the rat class I alcohol dehydrogenase gene

1991 ◽  
Vol 266 (23) ◽  
pp. 15457-15463 ◽  
Author(s):  
J.J. Potter ◽  
D. Cheneval ◽  
C.V. Dang ◽  
L.M. Resar ◽  
E. Mezey ◽  
...  
Keyword(s):  
Alcohol Dehydrogenase ◽  
Class I ◽  
Upstream Stimulatory Factor ◽  
Dehydrogenase Gene ◽  
Stimulatory Factor

Molecular phylogeny, species delimitation and biogeographic history of the Stegana (Steganina) shirozui species group (Diptera: Drosophilidae) from East Asia

2020 ◽  
Author(s):  
Ya-Lian Wang ◽  
Jin-Ming Lu ◽  
Yuan Zhang ◽  
Hong-Wei Chen
Keyword(s):  
Molecular Phylogeny ◽  
East Asia ◽  
Species Delimitation ◽  
Time Estimation ◽  
Species Group ◽  
28S Rrna ◽  
West China ◽  
Divergence Time Estimation ◽  
Biogeographic History ◽  
South West

Abstract The Stegana (Steganina) shirozui species group is mainly distributed in East Asia. In the present study, the molecular phylogeny of the S. shirozui group was investigated based on mitochondrial (COI and ND2) and nuclear (28S rRNA) markers. The resulting trees support the S. shirozui group as monophyletic and indicate that in this group, species associated with closer affinities show higher structural homogeneity in male genitalia. Molecular species delimitation assess most species limits and recognize four new species in the S. shirozui group from south-west China: S. alianya sp. nov., S. diodonta sp. nov., S. zebromyia sp. nov. and S. zopheria sp. nov. One new synonym was also recognized. Additionally, three typical male genital characters of the S. shirozui group were placed on the molecular phylogenetic framework. The outcome of both divergence-time estimation and ancestral area reconstruction suggests that the S. shirozui group likely originated in south-west China in the Middle Miocene.

scholarly journals trans activation of human alcohol dehydrogenase gene expression in hepatoma cells by C/EBP molecules bound in a novel arrangement just 5' and 3' to the TATA box.

1990 ◽  
Vol 10 (9) ◽  
pp. 5007-5010 ◽  
Author(s):  
M J Stewart ◽  
M L Shean ◽  
G Duester
Keyword(s):  
Alcohol Dehydrogenase ◽  
Hepatoma Cell ◽  
Promoter Sequence ◽  
Tata Box ◽  
Hepatoma Cell Line ◽  
Human Hepatoma Cell ◽  
Base Pairs ◽  
Enhancer Binding Protein ◽  
Dehydrogenase Gene ◽  
Adh2 Promoter

A promoter sequence between nucleotide -51 and nucleotide -10 in the human alcohol dehydrogenase gene ADH2 has been shown to bind the transcription factor CCAAT/enhancer-binding protein (C/EBP). A series of 5'-end deletions of the ADH2 promoter was cotransfected with a C/EBP expression plasmid in a human hepatoma cell line, and trans activation by C/EBP was seen when at least 171 base pairs of 5'-flanking DNA was present. Mutations in the ADH2 promoter indicate that the mechanism of C/EBP trans activation involves two binding sites, one located just upstream of the TATA box and one located in an unusual location between the TATA box and the transcription start point.

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