scholarly journals 96 Sublingual allergen-specific immunotherapy in pediatric patients: biomarkers and molecular allergy diagnostics as predictors of therapeutic success

2020 ◽  
Vol 25 (Supplement_2) ◽  
pp. e40-e40
Author(s):  
Lala Allahverdiyeva ◽  
G E Agaragimova ◽  
Veronica Mugarab Samedi
Keyword(s):  
House Dust ◽  
Pediatric Patients ◽  
Specific Immunotherapy ◽  
Dermatophagoides Pteronyssinus ◽  
House Dust Mites ◽  
Healthy Children ◽  
Dust Mites ◽  
Dermatophagoides Farinae ◽  
Allergen Specific Immunotherapy ◽  
Molecular Allergy

Abstract Background Efficacy of allergen-specific immunotherapy (ASIT) in patients with asthma could be significantly enhanced when physicians have an opportunity to identify those who are most likely to respond, determine when to stop treatment, and predict relapse. Thus, molecular allergy diagnostic is an effective tool that could help to individualize the diagnosis and treatment of asthma by accurate detection of the causative and cross-reactive allergens in pediatric patients. Cytokines are known as reliable diagnostic and prognostic biomarkers for asthma and can be used to evaluate the therapeutic effect. Objectives To evaluate the roles of molecular allergy diagnostic and biomarkers (L-10, IFNγ и TGFβ) as predictors of ASIT efficacy in pediatric patients with asthma. Design/Methods Eighty children aged 5 to 18 years with mild and moderate atopic bronchial asthma were included in the study. Sixty-eight out of 80 (85%) were found to have sensitization to house dust mites Dermatophagoides Pteronyssinus and/or Dermatophagoides Farinae. All these patients underwent molecular diagnostics using ImmunoCAP (Phadia 100) to determine the major (r Der p1, r Der p 2) and minor allergens (r Der p10) of the house dust mites. Twenty-eight patients with mild and moderate asthma were found to have a high sensitivity to these allergens and received SLIT with a mixture of house dust mites (Dermatophagoides Pteronyssinus and/or Dermatophagoides Farinae). To compare the efficacy of SLIT, we evaluated the severity of asthma clinical symptoms on a point scale from 0 to 3, and biomarker levels (IL-10, IFNγ и TGFβ) before and 6 months after SLIT. Twelve healthy children were included in the study as a reference group. Results At the beginning of the study, the average daily score in the SLIT group of 2.5 ± 0.7, and after 6 months of treatment, it decreased to 0.5 ± 0.2. The levels of biomarkers were markedly decreased prior to the treatment, especially TGFβ levels, and normalized at the end of treatment (Tab.1). Conclusion These findings support the efficacy of molecular diagnosis to determine the efficacy of allergen-specific immunotherapy in patients with asthma.

scholarly journals THE CLINICAL EFFICACY OF SUBLINGUAL ALLERGEN-SPECIFIC IMMUNOTHERAPY IN CHILDREN AGED 3-5 YEARS

2016 ◽  
Vol 6 ◽  
pp. 3-9
Author(s):  
Elena Sharikadze ◽  
Elena Okhotnikova ◽  
Serhii Yuriev
Keyword(s):  
House Dust ◽  
Specific Immunotherapy ◽  
Dermatophagoides Pteronyssinus ◽  
Information Value ◽  
House Dust Mites ◽  
In Vitro Tests ◽  
Dust Mites ◽  
Allergen Specific Immunotherapy

The aim of this study was to determine the efficacy of sublingual allergen–specific immunotherapy (SLIT) in Ukrainian children younger than 5 years old with allergic rhinitis and bronchial asthma sensitized to house dust mite allergens. Material and methods: Four hundred and fifty children aged 28 months up to 5 years with rhinitis or asthma were examined. One hundred and twenty five children sensitized to house dust mites Dermatophagoides pteronyssinus and/or Dermatophagoides farina were included. In vivo and in vitro tests were made with a standard inhalant allergens panel. Results: The high information value of molecular diagnostics methods applied prior to prescription of the given therapy in children is analyzed. It has been found that in children under 5 sensitized to allergens of house dust mites Dermatophagoides pteronyssinus and/or Dermatophagoides farinae the application of sublingual allergen–specific immunotherapy therapy allows gaining control over the symptoms of the disease during the first 6 months. Conclusion: The high safety of SLIT in children has been proven. Comparative analysis in the group of patients not receiving SLIT shows a high frequency of symptoms of the disease after “free-of-symptoms interval” against full or partial baseline therapy denial.

The Influence of Indoor Environmental Factors on the Prevalence of House Dust Mites as Aeroallergens in Bandar Abbas Student Dormitories, South of Iran, 2019

2021 ◽  
Author(s):  
Mehdi Zare ◽  
Mehdi Hassani-Azad ◽  
Moussa Soleimani-Ahmadi ◽  
Raziea Majnoon
Keyword(s):  
Environmental Factors ◽  
House Dust ◽  
Dermatophagoides Pteronyssinus ◽  
House Dust Mites ◽  
Dust Mites ◽  
Dermatophagoides Farinae ◽  
Floor Covering ◽  
The Mean ◽  
Bandar Abbas ◽  
Cheyletus Malaccensis

Abstract This study was conducted to determine the influence of environmental factors on the prevalence of house dust mites in student dormitories of Bandar Abbas city. In this study, 64 dust samples were collected from seven randomly selected dormitories located in various areas of the Bandar Abbas. The collected mites were isolated and mounted in Hoyer’s medium and identified using a morphological key. The associations between the environmental factors and the density of house dust mites were investigated. In total, 1,093 adult mites were collected and identified. They consisted of four species including Dermatophagoides pteronyssinus Trouessart (57.6%), Dermatophagoides farinae Hughes (24.3%) and Dermatophagoides evansi Fain (14.9%) (Acari: Pyroglyphidae), and Cheyletus malaccensis Oudemans (3.2%) (Acari: Cheyletidae). All of the dormitories were contaminated by more than one house dust mites species and the mean density of house dust mites in dormitories was 8.3 ± 0.2 mites/g of dust. There was a significant relationship between average house dust mites density and some of environmental factors such as relative humidity, temperature, floor covering type, and number of occupants (P < 0.05). Results of this study revealed that two major allergenic dust mites, D. pteronyssinus and D. farinae, were the most prevalent and collected from all of dormitories and some of indoor environmental factors found to influence mites’ population.

scholarly journals IgE-dependent reactivity of canine sera from three regions of Poland

2020 ◽  
Vol 76 (06) ◽  
pp. 6410-2020
Author(s):  
BARTOSZ JANIA ◽  
MARTA DOPIERAŁA ◽  
EWA WÓJCIK ◽  
KATARZYNA RYMUZA ◽  
KATARZYNA ANDRASZEK
Keyword(s):  
House Dust ◽  
Genetic Predisposition ◽  
Dermatophagoides Pteronyssinus ◽  
House Dust Mites ◽  
Allergic Reactions ◽  
Dust Mites ◽  
Dermatophagoides Farinae ◽  
Acarus Siro ◽  
Study Support ◽  
Health Complications

Allergies in dogs have become a relatively frequent topic of discussion in veterinary surgeries. Many dogs, both purebred and of mixed breeds, are diagnosed for allergies. Allergies have many causes, from genetic predisposition to the environmental conditions in which the animal lives. Allergies in dogs are troublesome because they can lead to serious health complications when diagnosed late or left untreated. The results of the study support the hypothesis that the percentage of allergies to individual allergens varied by region (Tab. 4). Differences were observed in the case of Dermatophagoides pteronyssinus and Dermatophagoides farinae house dust mites, Acarus siro, and Tyrophagus. The remaining allergens caused allergic reactions in similar percentages of dogs from northern, central and southern Poland.

scholarly journals House Dust Mites in One of Indonesian Detention Center in 2020

Biomedika ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 98-103
Author(s):  
Ajrina Malia ◽  
Makhabbah Jamilatun ◽  
Aminah Aminah
Keyword(s):  
House Dust ◽  
Descriptive Study ◽  
Dermatophagoides Pteronyssinus ◽  
House Dust Mites ◽  
Dust Mites ◽  
Dermatophagoides Farinae ◽  
Detention Center ◽  
Home Furnishings ◽  
Dust Mite ◽  
Flotation Method

House dust mite was one of the most common allergens in homes around the world and associated with manifestations of allergies of skin and respiratory tract such as bronchial asthma, allergic rhinitis, and atopic dermatitis. This arthropod was commonly found on carpets, mattresses, children's toys, and other home furnishings. This study was aimed to identify and quantify the number of Dermatophagoides spp in Detention Center. This descriptive study was conducted using flotation method on 52 samples of mattress dust collected in February 2020. The results showed that 49 out of 52 mattress dust samples were positive of Dermatophagoidesspp..Of all Dermatophagoides spp, there were Dermatophagoides pteronyssinus (71.2%) and Dermatophagoides farinae (28.8%). In conclusion, this study found two species of Dermatophagoides spp. in Detention Center and suggested that the fascility maintain the cleanliness of the mattress by drying the mattress and changing the sheets at least once a week.

scholarly journals Immunoglobulin G1 subclass responses can be used to detect specific allergy to the house dust mites Dermatophagoides farinae and Dermatophagoides pteronyssinus in atopic dogs

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
N. Khantavee ◽  
C. Chanthick ◽  
A. Tungtrongchitr ◽  
N. Techakriengkrai ◽  
S. Suradhat ◽  
...  
Keyword(s):  
House Dust ◽  
Characteristic Curve ◽  
False Positive Rate ◽  
Specific Ige ◽  
House Dust Mites ◽  
Dust Mites ◽  
Dermatophagoides Farinae ◽  
Allergen Specific Immunotherapy ◽  
Intradermal Testing ◽  
Positive Rate

Abstract Background In dogs with atopic dermatitis, intradermal testing (IDT) or allergen specific IgE serological testing are routinely employed to identify causative allergens. These allergens can then be used for allergen-specific immunotherapy and allergy management. The clinical relevance of this testing is affected by the source of allergen, and other biomarkers that are more related to specific allergens still need to be identified. The aim of this study was to investigate levels of specific IgE, total IgG, and IgG1 and IgG2 subclasses against the local house dust mites (HDM) Dermatophagoides farinae (DF) and D. pteronyssinus (DP) as biomarkers by using in-house ELISAs in healthy (n = 33) and atopic dogs (AD) (n = 44) that were either positive or negative by IDT to HDM. Results Being over 3 years of age was a risk factor for AD (Odds Ratio (OD) = 4.10, 95% Confidence interval (CI) 1.57–10.75, p = 0.0049), but there was no relation to IDT outcomes (OR = 0.9091, 95% CI 0.22–3.74, p = 1.00). High levels of all antibody isotypes (IgE, IgG, IgG1 and IgG2) against HDM were found in aged healthy dogs (> 3 years old). In AD, HDM-IgE and IgG1 levels were higher in dogs that were IDT positive to HDM than in IDT negative animals. Levels of IgE and IgG1 could be used to distinguish the specific allergens, whereas total IgG and IgG2 levels were not different between IDT-positive and IDT-negative AD. By the receiver operating characteristic curve at a false-positive rate = 0.10, both IgE and IgG1 showed better sensitivity than IgG and IgG2. Similar to IgE, serum IgG1 concentration was also relevant to IDT outcomes. Conclusions Our in-house ELISAs coated with local HDM were useful for evaluating antibody levels, and we propose use of the HDM-specific IgG1 subclass as a biomarker to detect HDM specific allergens in AD, potentially together with an IgE based platform.

scholarly journals Patient dropouts from sublingual allergen specific immunotherapy with house dust mites. Solving a problem.

2020 ◽  
Vol 17 (2) ◽  
pp. 53-60
Author(s):  
Olga V. Trusova ◽  
Andrey V. Kamaev ◽  
Irina V. Makarova
Keyword(s):  
House Dust ◽  
Patient Management ◽  
Specific Immunotherapy ◽  
Low Frequency ◽  
Management Plan ◽  
Drop Out ◽  
House Dust Mites ◽  
Treatment Withdrawal ◽  
Dust Mites ◽  
Allergen Specific Immunotherapy

Relevance. The insufficient effect of sublingual allergen-specific immunotherapy (SLIT) is caused, first of all, by non-compliance with the treatment regimen and premature treatment termination. Purpose of the study. Determining the frequency of patient drop-out rate during SLIT with house dust mites (HDM) allergens in children with allergic rhinitis (AR) or AR in combination with bronchial asthma (BA), with an analysis of the drop-out reasons, and approbation of the developed visit-to-visit patient management plan (Plan). Materials and methods. We analyzed 274 cases of treatment with HDM SLIT in children. 218 patients: 67.4% (147) boys, median age 11.33 years [7.26; 15.46], the proportion of patients with BA 43.1% (94 children) received HDM SLIT in 20132020. 56 patients: 71.4% (40) boys, median age 9.29 years [6.13; 15.93], the proportion of patients with BA 78.6% (44 children) received treatment in accordance with the Plan. Results. A relatively low frequency of treatment withdrawal was noted in the first 2 years of therapy (2 years after the start of treatment, 72.47% patients continue it). However, only 52.29% complete 3 years of therapy, and 14.67% complete 4 years of therapy. Implementation of the Plan increased patient retention in treatment at the 3rd year of treatment to 69.64% (p=0.031). Conclusions. Only half of the patients receive the required three-year minimum of treatment. The daily plan optimizes the patient management schedule for HDM ASIT; reduces patient dropout from treatment and can be recommended for practical healthcare.

scholarly journals HUBUNGAN KEPADATAN DERMATOPHAGOIDES SPP. DENGAN FREKUENSI SERANGAN ASMA PADA PENDERITA ASMA DI RS PARU JEMBER

2019 ◽  
Vol 19 (2) ◽  
Author(s):  
Denaneer Rahmadatu ◽  
Erma Sulistyaningsih ◽  
Dini Agustina
Keyword(s):  
House Dust ◽  
Random Sampling ◽  
Simple Random Sampling ◽  
Dermatophagoides Pteronyssinus ◽  
House Dust Mites ◽  
Inflammatory Disorder ◽  
Dust Mites ◽  
Dermatophagoides Farinae ◽  
Cross Sectional ◽  
Asthmatic Patients

Abstrak. Asma merupakan gangguan inflamasi kronik saluran napas yang bersifat hiperesponsif yang ditandai adanya gejala episodik berulang berupa mengi, sesak napas, dada terasa berat, dan batuk, terutama pada malam hari atau dini hari. Sekitar 50-80% asma disebabkan oleh tungau debu rumah (TDR), khususnya Dermatophagoides pteronyssinus dan Dermatophagoides farinae. Penelitian ini bertujuan untuk mengetahui apakah terdapat hubungan antara kepadatan Dermatophagoides spp. pada debu rumah dengan frekuensi serangan asma pada penderita asma di RS Paru Jember. Penelitian ini merupakan penelitian analitik observasional dengan desain penelitian cross-sectional. Sampel penelitian ini yaitu 30 penderita asma di RS Paru Jember yang diambil menggunakan teknik simple random sampling sesuai dengan kriteria inklusi dan eksklusi. Peneliti melakukan interview mengenai frekuensi serangan asma dalam tiga bulan terakhir, mengambil debu dari ruang tamu dan ruang tidur pada rumah penderita asma, dan mengidentifikasi tungau debu rumah (TDR) yang ditemukan pada debu. Hasil uji Pearson mendapatkan nilai p=0,011 dan nilai r=0,475 sehingga dapat disimpulkan bahwa terdapat hubungan bermakna antara kepadatan Dermatophagoides spp. dengan frekuensi serangan asma dengan kekuatan korelasi sedang pada penderita asma di RS Paru Jember. Kata Kunci: Dermatophagoides spp., Tungau Debu Rumah (TDR), Asma Abstract. Asthma is a chronic inflammatory disorder of the respiratory system characterized by recurrent episodes of wheezing, shortness of breath, chest tightness, and cough, particularly at night or early morning. About 50-80% of asthma is caused by house dust mite (HDM), especially Dermatophagoides pteronyssinus and Dermatophagoides farinae. This study aimed to investigate the correlation between the density of Dermatophagoides spp. and the frequency of asthma attacks in asthmatic patients at Jember Pulmonary Hospital. This study was an observational analytic study with a cross-sectional design. Respondents were 30 asthmatic patients at Jember Pulmonary Hospital matched with inclusion and exclusion criteria and chose by simple random sampling. Respondents were interviewed about the frequency of asthma attacks in the last three month,s and the hose dust from the living and sleeping rooms were collected for identification of house dust mites (HDM). The pearson test showed a value of p=0.011 and r=0.475. It can be concluded that there is a significant correlation with the moderate strenght between the density of Dermatophagoides spp. and the frequency of asthma attacks in the asthmatic patient at Jember Pulmonary Hospital. Keywords: Dermatophagoides spp., House Dust Mites (HDM), Asthma

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