Reactive Carbonyl Species Mediate Methyl Jasmonate-Induced Stomatal Closure

2020 ◽  
Vol 61 (10) ◽  
pp. 1788-1797
Author(s):  
Md. Moshiul Islam ◽  
Wenxiu Ye ◽  
Fahmida Akter ◽  
Mohammad Saidur Rhaman ◽  
Daiki Matsushima ◽  
...  
Keyword(s):  
Methyl Jasmonate ◽  
Stomatal Closure ◽  
Guard Cells ◽  
H2o2 Production ◽  
Aba Signaling ◽  
Tobacco Plants ◽  
Reactive Carbonyl Species ◽  
In The Wild ◽  
Carbonyl Species ◽  
Reactive Carbonyl

Abstract Production of reactive oxygen species (ROS) is a key signal event for methyl jasmonate (MeJA)- and abscisic acid (ABA)-induced stomatal closure. We recently showed that reactive carbonyl species (RCS) stimulates stomatal closure as an intermediate downstream of hydrogen peroxide (H2O2) production in the ABA signaling pathway in guard cells of Nicotiana tabacum and Arabidopsis thaliana. In this study, we examined whether RCS functions as an intermediate downstream of H2O2 production in MeJA signaling in guard cells using transgenic tobacco plants overexpressing A. thaliana 2-alkenal reductase (n-alkanal + NAD(P)+ ⇌ 2-alkenal + NAD(P)H + H+) (AER-OE tobacco) and Arabidopsis plants. The stomatal closure induced by MeJA was impaired in the AER-OE tobacco and was inhibited by RCS scavengers, carnosine and pyridoxamine, in the wild-type (WT) tobacco plants and Arabidopsis plants. Application of MeJA significantly induced the accumulation of RCS, including acrolein and 4-hydroxy-(E)-2-nonenal, in the WT tobacco but not in the AER-OE plants. Application of MeJA induced H2O2 production in the WT tobacco and the AER-OE plants and the H2O2 production was not inhibited by the RCS scavengers. These results suggest that RCS functions as an intermediate downstream of ROS production in MeJA signaling and in ABA signaling in guard cells.

scholarly journals Reactive Carbonyl Species Mediate ABA Signaling in Guard Cells

2016 ◽  
Vol 57 (12) ◽  
pp. 2552-2563 ◽  
Author(s):  
Md. Moshiul Islam ◽  
Wenxiu Ye ◽  
Daiki Matsushima ◽  
Shintaro Munemasa ◽  
Eiji Okuma ◽  
...  
Keyword(s):  
Guard Cells ◽  
Aba Signaling ◽  
Reactive Carbonyl Species ◽  
Carbonyl Species ◽  
Reactive Carbonyl

scholarly journals The Myrosinases TGG1 and TGG2 Function Redundantly in Reactive Carbonyl Species Signaling in Arabidopsis Guard Cells

2020 ◽  
Vol 61 (5) ◽  
pp. 967-977 ◽  
Author(s):  
Mohammad Saidur Rhaman ◽  
Toshiyuki Nakamura ◽  
Yoshimasa Nakamura ◽  
Shintaro Munemasa ◽  
Yoshiyuki Murata
Keyword(s):  
Stomatal Closure ◽  
Guard Cells ◽  
Signal Pathway ◽  
Ethylene Glycol Tetraacetic Acid ◽  
Free Calcium ◽  
Ros Production ◽  
Reactive Carbonyl Species ◽  
Aldehydes And Ketones ◽  
Carbonyl Species ◽  
Reactive Carbonyl

Abstract Myrosinase (β-thioglucoside glucohydrolase, enzyme nomenclature, EC 3.2.1.147, TGG) is a highly abundant protein in Arabidopsis guard cells, of which TGG1 and TGG2 function redundantly in abscisic acid (ABA)- and methyl jasmonate-induced stomatal closure. Reactive carbonyl species (RCS) are α,β-unsaturated aldehydes and ketones, which function downstream of reactive oxygen species (ROS) production in the ABA signalling pathway in guard cells. Among the RCS, acrolein is the most highly reactive, which is significantly produced in ABA-treated guard cells. To clarify the ABA signal pathway downstream of ROS production, we investigated the responses of tgg mutants (tgg1-3, tgg2-1 and tgg1-3 tgg2-1) to acrolein. Acrolein induced stomatal closure and triggered cytosolic alkalization in wild type (WT), tgg1-3 single mutants and in tgg2-1 single mutants, but not in tgg1-3 tgg2-1 double mutants. Exogenous Ca2+ induced stomatal closure and cytosolic alkalization not only in WT but also in all of the mutants. Acrolein- and Ca2+-induced stomatal closures were inhibited by an intracellular acidifying agent, butyrate, a Ca2+ chelator, ethylene glycol tetraacetic acid (EGTA) and a Ca2+ channel blocker, LaCl3. Acrolein induced cytosolic free calcium concentration ([Ca2+]cyt) elevation in guard cells of WT plants but not in the tgg1-3 tgg2-1 double mutants. Exogenous Ca2+ elicited [Ca2+]cyt elevation in guard cells of WT and tgg1-3 tgg2-1. Our results suggest that TGG1 and TGG2 function redundantly, not between ROS production and RCS production, but downstream of RCS production in the ABA signal pathway in Arabidopsis guard cells.

Reactive Carbonyl Species Function as Signal Mediators Downstream of H2O2 Production and Regulate [Ca2+]cyt Elevation in ABA Signal Pathway in Arabidopsis Guard Cells

2019 ◽  
Vol 60 (5) ◽  
pp. 1146-1159 ◽  
Author(s):  
Md. Moshiul Islam ◽  
Wenxiu Ye ◽  
Daiki Matsushima ◽  
Mohammad Saidur Rhaman ◽  
Shintaro Munemasa ◽  
...  
Keyword(s):  
Guard Cells ◽  
Signal Pathway ◽  
H2o2 Production ◽  
Reactive Carbonyl Species ◽  
Signal Mediators ◽  
Carbonyl Species ◽  
Reactive Carbonyl

scholarly journals Ethylene Inhibits Methyl Jasmonate-Induced Stomatal Closure by Modulating Guard Cell Slow-Type Anion Channel Activity via the OPEN STOMATA 1/SnRK2.6 Kinase-Independent Pathway in Arabidopsis

2019 ◽  
Vol 60 (10) ◽  
pp. 2263-2271 ◽  
Author(s):  
Shintaro Munemasa ◽  
Yukari Hirao ◽  
Kasumi Tanami ◽  
Yoshiharu Mimata ◽  
Yoshimasa Nakamura ◽  
...  
Keyword(s):  
Methyl Jasmonate ◽  
Guard Cell ◽  
Stomatal Closure ◽  
Guard Cells ◽  
Defense Responses ◽  
Ros Production ◽  
Ethylene Signaling ◽  
Aba Signaling ◽  
Anion Channels ◽  
Signal Crosstalk

Abstract Signal crosstalk between jasmonate and ethylene is crucial for a proper maintenance of defense responses and development. Although previous studies reported that both jasmonate and ethylene also function as modulators of stomatal movements, the signal crosstalk mechanism in stomatal guard cells remains unclear. Here, we show that the ethylene signaling inhibits jasmonate signaling as well as abscisic acid (ABA) signaling in guard cells of Arabidopsis thaliana and reveal the signaling crosstalk mechanism. Both an ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) and an ethylene-releasing compound ethephon induced transient stomatal closure, and also inhibited methyl jasmonate (MeJA)-induced stomatal closure as well as ABA-induced stomatal closure. The ethylene inhibition of MeJA-induced stomatal closure was abolished in the ethylene-insensitive mutant etr1–1, whereas MeJA-induced stomatal closure was impaired in the ethylene-overproducing mutant eto1–1. Pretreatment with ACC inhibited MeJA-induced reactive oxygen species (ROS) production as well as ABA-induced ROS production in guard cells but did not suppress ABA activation of OPEN STOMATA 1 (OST1) kinase in guard cell-enriched epidermal peels. The whole-cell patch-clamp analysis revealed that ACC attenuated MeJA and ABA activation of S-type anion channels in guard cell protoplasts. However, MeJA and ABA inhibitions of Kin channels were not affected by ACC pretreatment. These results suggest that ethylene signaling inhibits MeJA signaling and ABA signaling by targeting S-type anion channels and ROS but not OST1 kinase and K+ channels in Arabidopsis guard cells.

Hydrogen sulfide induced by hydrogen peroxide mediates brassinosteroid-induced stomatal closure of Arabidopsis thaliana

2021 ◽  
Vol 48 (2) ◽  
pp. 195 ◽  
Author(s):  
Yinli Ma ◽  
Luhan Shao ◽  
Wei Zhang ◽  
Fengxi Zheng
Keyword(s):  
Hydrogen Peroxide ◽  
Arabidopsis Thaliana ◽  
Hydrogen Sulfide ◽  
Stomatal Closure ◽  
Guard Cells ◽  
H2o2 Production ◽  
Wild Type ◽  
Synthesis Inhibitor ◽  
In The Wild ◽  
H2s Production

The role of hydrogen sulfide (H2S) and its relationship with hydrogen peroxide (H2O2) in brassinosteroid-induced stomatal closure in Arabidopsis thaliana (L.) Heynh. were investigated. In the present study, 2,4-epibrassinolide (EBR, a bioactive BR) induced stomatal closure in the wild type, the effects were inhibited by H2S scavenger and synthesis inhibitors, and H2O2 scavengers and synthesis inhibitor. However, EBR failed to close the stomata of mutants Atl-cdes, Atd-cdes, AtrbohF and AtrbohD/F. Additionally, EBR induced increase of L-/D-cysteine desulfhydrase (L-/D-CDes) activity, H2S production, and H2O2 production in the wild type, and the effects were inhibited by H2S scavenger and synthesis inhibitors, and H2O2 scavengers and synthesis inhibitor respectively. Furthermore, EBR increased H2O2 levels in the guard cells of AtrbohD mutant, but couldn’t raise H2O2 levels in the guard cells of AtrbohF and AtrbohD/F mutants. Next, scavengers and synthesis inhibitor of H2O2 could significantly inhibit EBR-induced rise of L-/D-CDes activity and H2S production in the wild type, but H2S scavenger and synthesis inhibitors failed to repress EBR-induced H2O2 production. EBR could increase H2O2 levels in the guard cells of Atl-cdes and Atd-cdes mutants, but EBR failed to induce increase of L-/D-CDes activity and H2S production in AtrbohF and AtrbohD/F mutants. Therefore, we conclude that H2S and H2O2 are involved in the signal transduction pathway of EBR-induced stomatal closure. Altogether, our data suggested that EBR induces AtrbohF-dependent H2O2 production and subsequent AtL-CDes-/AtD-CDes-catalysed H2S production, and finally closes stomata in A. thaliana.

scholarly journals Insoluble dietary fibre scavenges reactive carbonyl species under simulated physiological conditions: The key role of fibre-bound polyphenols

2021 ◽  
Vol 349 ◽  
pp. 129018
Author(s):  
Hao Zhang ◽  
Antonio Dario Troise ◽  
Yajing Qi ◽  
Gangcheng Wu ◽  
Hui Zhang ◽  
...  
Keyword(s):  
Dietary Fibre ◽  
Physiological Conditions ◽  
Reactive Carbonyl Species ◽  
Insoluble Dietary Fibre ◽  
Carbonyl Species ◽  
Reactive Carbonyl

scholarly journals Detoxification of Reactive Carbonyl Species by Glutathione Transferase Tau Isozymes

2019 ◽  
Vol 10 ◽  
Author(s):  
Jun’ichi Mano ◽  
Sayaka Kanameda ◽  
Rika Kuramitsu ◽  
Nagisa Matsuura ◽  
Yasuo Yamauchi
Keyword(s):  
Glutathione Transferase ◽  
Reactive Carbonyl Species ◽  
Carbonyl Species ◽  
Reactive Carbonyl

scholarly journals Reactive Carbonyl Species and Protein Adducts: Identification Strategies, Biological Mechanisms and Molecular Approaches for Their Detoxification

Antioxidants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 690
Author(s):  
Giancarlo Aldini ◽  
Alessandra A. Altomare
Keyword(s):  
Protein Adducts ◽  
Special Issue ◽  
Biological Mechanisms ◽  
Research Papers ◽  
Molecular Approaches ◽  
Reactive Carbonyl Species ◽  
Carbonyl Species ◽  
Reactive Carbonyl

The Special issue is composed of 13 contributions: 9 research papers and 4 reviews [...]

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