Electrophysiological Identification and Activity Analyses of Plasma Membrane K+ Channels in Maize Guard Cells

2018 ◽  
Vol 60 (4) ◽  
pp. 765-777
Author(s):  
Yong-Qiang Gao ◽  
Wei-Hua Wu ◽  
Yi Wang
Keyword(s):  
Plasma Membrane ◽  
Guard Cells ◽  
K Channels

scholarly journals K+ transport properties of K+ channels in the plasma membrane of Vicia faba guard cells.

1988 ◽  
Vol 92 (5) ◽  
pp. 667-683 ◽  
Author(s):  
J I Schroeder
Keyword(s):  
Plasma Membrane ◽  
Transport Properties ◽  
Vicia Faba ◽  
Guard Cell ◽  
Guard Cells ◽  
Stomatal Opening ◽  
Whole Cell ◽  
K Channels ◽  
K Transport ◽  
K Currents

Electrical properties of the plasma membrane of guard cell protoplasts isolated from stomates of Vicia faba leaves were studied by application of the whole-cell configuration of the patch-clamp technique. The two types of K+ currents that have recently been identified in guard cells may allow efflux of K+ during stomatal closing, and uptake of K+ during stomatal opening (Schroeder et al., 1987). A detailed characterization of ion transport properties of the inward-rectifying (IK+,in) and the outward-rectifying (IK+,out) K+ conductance is presented here. The permeability ratios of IK+,in and IK+,out currents for K+ over monovalent alkali metal ions were determined. The resulting permeability sequences (PK+ greater than PRb+ greater than PNa+ greater than PLi+ much greater than PCs+) corresponded closely to the ion specificity of guard cell movements in V. faba. Neither K+ currents exhibited significant inactivation when K+ channels were activated for prolonged periods (greater than 10 min). The absence of inactivation may permit long durations of K+ fluxes, which occur during guard cell movements. Activation potentials of inward K+ currents were not shifted when external K+ concentrations were changed. This differs strongly from the behavior of inward-rectifying K+ channels in animal tissue. Blue light and fusicoccin induce hyperpolarization by stimulation of an electrogenic pump. From slow-whole-cell recordings it was concluded that electrogenic pumps require cytoplasmic substrates for full activation and that the magnitude of the pump current is sufficient to drive K+ uptake through IK+,in channels. First, direct evidence was gained for the hypothesis that IK+,in channels are a molecular pathway for K+ accumulation by the finding that IK+,in was blocked by Al3+ ions, which are known to inhibit stomatal opening but not closing. The results presented in this study strongly support a prominent role for IK+,in and IK+,out channels in K+ transport across the plasma membrane of guard cells.

Extracellular Ca2+ Regulating Stomatal Movement and Plasma Membrane K+ Channels in Guard Cells of Vicia faba Under Salt Stress

2008 ◽  
Vol 34 (11) ◽  
pp. 1970-1976 ◽  
Author(s):  
Xiang ZHAO ◽  
Yan-Liang WANG ◽  
Ya-Jing WANG ◽  
Xi-Li WANG ◽  
Xiao ZHANG
Keyword(s):  
Plasma Membrane ◽  
Salt Stress ◽  
Vicia Faba ◽  
Guard Cells ◽  
Stomatal Movement ◽  
K Channels

The phenomenon of solar tracking is known from the early studies of Yin (1938) and that it is a blue light response, while the actual photoreceptors involved are not identified (Batschauer 1998; Briggs and Christie, 2002). In view of the fact that solar tracking behaviour results from light-driven turgor changes in the volume of the cells just as in the case of stomatal guard cells, the phototropins might be involved here also (Briggs and Christie, 2002). Sakamoto and Briggs (2002) have suggested that solar tracking may involv e phototropins. The precise molecular mechanisms concernin g the heliotropic movements are not currently known , while some knowledge is available on the mechanism of nyctinastic movements. However, Cronlund and Forseth (1995) have studied the mechanism of soybean leaflet movement and concluded that the mechanism of heliotropic movement was similar to that of nyctinastic movements. These authors have studied the role of K+ channels and the plasma membrane H/ATPase (Michelet and Boutry, 1995) in paraheliotropic movements throug h the measurements of leaf movements after treatment of pulvinus with promoters and inhibitors of HATPase and K+ channels. HATPase inhibition reduced the leaf

2004 ◽  
pp. 101-101
Keyword(s):  
Plasma Membrane ◽  
Molecular Mechanisms ◽  
Light Response ◽  
Guard Cells ◽  
K Channels ◽  
Solar Tracking ◽  
Blue Light Response ◽  
Leaf Movements ◽  
Stomatal Guard Cells

Modulation of frequency and height of cytosolic calcium spikes by plasma membrane anion channels in guard cells

2021 ◽  
Author(s):  
Md Tahjib-Ul-Arif ◽  
Shintaro Munemasa ◽  
Toshiyuki Nakamura ◽  
Yoshimasa Nakamura ◽  
Yoshiyuki Murata
Keyword(s):  
Plasma Membrane ◽  
Stomatal Closure ◽  
Anion Channel ◽  
Guard Cells ◽  
Cytosolic Calcium ◽  
Peak Height ◽  
Extracellular Calcium ◽  
Wild Type ◽  
Anion Channels ◽  
In The Wild

Abstract Cytosolic calcium ([Ca2+]cyt) elevation activates plasma membrane anion channels in guard cells, which is required for stomatal closure. However, involvement of the anion channels in the [Ca2+]cyt elevation remains unclear. We investigated the involvement using Arabidopsis thaliana anion channel mutants, slac1-4 slah3-3 and slac1-4 almt12-1. Extracellular calcium induced stomatal closure in the wild-type plants but not in the anion channel mutant plants whereas extracellular calcium induced [Ca2+]cyt elevation both in the wild-type guard cells and in the mutant guard cells. The peak height and the number of the [Ca2+]cyt spike were lower and larger in the slac1-4 slah3-3 than in the wild-type and the height and the number in the slac1-4 almt12-1 were much lower and much larger than in the wild-type. These results suggest that the anion channels are involved in the regulation of [Ca2+]cyt elevation in guard cells.

Actin filaments regulate the adhesion between the plasma membrane and the cell wall of tobacco guard cells

PROTOPLASMA ◽  
2017 ◽  
Vol 255 (1) ◽  
pp. 235-245 ◽  
Author(s):  
Qin Yu ◽  
Jing-Jing Ren ◽  
Lan-Jing Kong ◽  
Xiu-Ling Wang
Keyword(s):  
Cell Wall ◽  
Plasma Membrane ◽  
Actin Filaments ◽  
Guard Cells

scholarly journals Overexpression of Plasma Membrane H+-ATPase in Guard Cells Enhances Light-Induced Stomatal Opening, Photosynthesis, and Plant Growth in Hybrid Aspen

2021 ◽  
Vol 12 ◽  
Author(s):  
Shigeo Toh ◽  
Naoki Takata ◽  
Eigo Ando ◽  
Yosuke Toda ◽  
Yin Wang ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Transgenic Plants ◽  
Woody Plants ◽  
Stem Elongation ◽  
Guard Cells ◽  
Gus Expression ◽  
Stomatal Opening ◽  
Wild Type ◽  
Exchange System ◽  
Gas Exchange System

Stomata in the plant epidermis open in response to light and regulate CO2 uptake for photosynthesis and transpiration for uptake of water and nutrients from roots. Light-induced stomatal opening is mediated by activation of the plasma membrane (PM) H+-ATPase in guard cells. Overexpression of PM H+-ATPase in guard cells promotes light-induced stomatal opening, enhancing photosynthesis and growth in Arabidopsis thaliana. In this study, transgenic hybrid aspens overexpressing Arabidopsis PM H+-ATPase (AHA2) in guard cells under the strong guard cell promoter Arabidopsis GC1 (AtGC1) showed enhanced light-induced stomatal opening, photosynthesis, and growth. First, we confirmed that AtGC1 induces GUS expression specifically in guard cells in hybrid aspens. Thus, we produced AtGC1::AHA2 transgenic hybrid aspens and confirmed expression of AHA2 in AtGC1::AHA2 transgenic plants. In addition, AtGC1::AHA2 transgenic plants showed a higher PM H+-ATPase protein level in guard cells. Analysis using a gas exchange system revealed that transpiration and the photosynthetic rate were significantly increased in AtGC1::AHA2 transgenic aspen plants. AtGC1::AHA2 transgenic plants showed a>20% higher stem elongation rate than the wild type (WT). Therefore, overexpression of PM H+-ATPase in guard cells promotes the growth of perennial woody plants.

The plasma membrane and tonoplast of guard cells

1992 ◽  
pp. 220-238 ◽  
Author(s):  
C. M. Willmer ◽  
O. Pantoja
Keyword(s):  
Plasma Membrane ◽  
Guard Cells
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