scholarly journals Engineered disulfide bonds in recombinant human interferon-γ: the impact of the N-terminal helix A and the AB-loop on protein stability

1996 ◽  
Vol 9 (10) ◽  
pp. 905-912 ◽  
Author(s):  
Gero Waschütza ◽  
Volkhart Li ◽  
Thomas Schäfer ◽  
Dietmar Schomburg ◽  
Carmen Villmann ◽  
...  
Keyword(s):  
Protein Stability ◽  
Disulfide Bonds ◽  
Interferon Γ ◽  
Human Interferon ◽  
The Impact

scholarly journals Crystal structure of human interferon-γ receptor 2 reveals the structural basis for receptor specificity

2016 ◽  
Vol 72 (9) ◽  
pp. 1017-1025 ◽  
Author(s):  
Pavel Mikulecký ◽  
Jirí Zahradník ◽  
Petr Kolenko ◽  
Jiří Černý ◽  
Tatsiana Charnavets ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Disulfide Bonds ◽  
Bioinformatic Analysis ◽  
Structural Features ◽  
Interferon Γ ◽  
Cell Surface Receptor ◽  
Structural Basis ◽  
Human Interferon ◽  
Multiple Sequence ◽  
Surface Receptor

Interferon-γ receptor 2 is a cell-surface receptor that is required for interferon-γ signalling and therefore plays a critical immunoregulatory role in innate and adaptive immunity against viral and also bacterial and protozoal infections. A crystal structure of the extracellular part of human interferon-γ receptor 2 (IFNγR2) was solved by molecular replacement at 1.8 Å resolution. Similar to other class 2 receptors, IFNγR2 has two fibronectin type III domains. The characteristic structural features of IFNγR2 are concentrated in its N-terminal domain: an extensive π–cation motif of stacked residues KWRWRH, a NAG–W–NAG sandwich (where NAG stands forN-acetyl-D-glucosamine) and finally a helix formed by residues 78–85, which is unique among class 2 receptors. Mass spectrometry and mutational analyses showed the importance of N-linked glycosylation to the stability of the protein and confirmed the presence of two disulfide bonds. Structure-based bioinformatic analysis revealed independent evolutionary behaviour of both receptor domains and, together with multiple sequence alignment, identified putative binding sites for interferon-γ and receptor 1, the ligands of IFNγR2.

Computer-aided modeling of structure stabilizing disulfide bonds in recombinant human interferon-γ

1996 ◽  
Vol 71 (1) ◽  
pp. 37-44
Author(s):  
Gero Günther ◽  
Till Fechteler ◽  
Carmen Villmann ◽  
Hayssam Zakaria ◽  
Dietmar Schomburg ◽  
...  
Keyword(s):  
Disulfide Bonds ◽  
Interferon Γ ◽  
Human Interferon ◽  
Computer Aided

scholarly journals INPS: predicting the impact of non-synonymous variations on protein stability from sequence

2015 ◽  
Vol 31 (17) ◽  
pp. 2816-2821 ◽  
Author(s):  
Piero Fariselli ◽  
Pier Luigi Martelli ◽  
Castrense Savojardo ◽  
Rita Casadio
Keyword(s):  
Protein Stability ◽  
The Impact

A point mutation that decreases the thermal stability of human interferon γ

1992 ◽  
Vol 5 (3) ◽  
pp. 249-252 ◽  
Author(s):  
C.A. Lunn ◽  
J. Fossetta ◽  
N. Murgolo ◽  
P. J. Zavodny ◽  
D. Lundell ◽  
...  
Keyword(s):  
Thermal Stability ◽  
Point Mutation ◽  
Interferon Γ ◽  
Human Interferon ◽  
Thermal Stability Of

scholarly journals Mouse Macrophages Carrying Both Subunits of the Human Interferon-γ (IFN-γ) Receptor Respond to Human IFN-γ but Do Not Acquire Full Protection against Viral Cytopathic Effect

1996 ◽  
Vol 271 (51) ◽  
pp. 32659-32666 ◽  
Author(s):  
David Lembo ◽  
Paola Ricciardi-Castagnoli ◽  
Gottfried Alber ◽  
Laurence Ozmen ◽  
Santo Landolfo ◽  
...  
Keyword(s):  
Cytopathic Effect ◽  
Interferon Γ ◽  
Human Interferon ◽  
Mouse Macrophages ◽  
Ifn Γ ◽  
Full Protection ◽  
Viral Cytopathic Effect

scholarly journals Biosynthetic analysis of the human interferon-γ receptor

1989 ◽  
Vol 264 (20) ◽  
pp. 11981-11988
Author(s):  
G K K Hershey ◽  
R D Schreiber
Keyword(s):  
Interferon Γ ◽  
Human Interferon

scholarly journals Human Interferon γ is encoded by a single class of mRNA

1982 ◽  
Vol 10 (12) ◽  
pp. 3605-3615 ◽  
Author(s):  
Rik Derynck ◽  
David W. Leung ◽  
Patrick W. Gray ◽  
David V. Goeddel
Keyword(s):  
Interferon Γ ◽  
Human Interferon ◽  
Single Class

Effects of annealing lyophilized and spray‐lyophilized formulations of recombinant human interferon‐γ

2003 ◽  
Vol 92 (4) ◽  
pp. 715-729 ◽  
Author(s):  
Serena D. Webb ◽  
Jeffrey L. Cleland ◽  
John F. Carpenter ◽  
Theodore W. Randolph
Keyword(s):  
Interferon Γ ◽  
Human Interferon

The recombinant human interferon-γ receptor is fully functional in a human × murine hybrid containing human chromosome 21

1991 ◽  
Vol 142 (9) ◽  
pp. 765-772 ◽  
Author(s):  
M.R. Bono ◽  
C. Alcaïde-Loridan ◽  
B. Letouzé ◽  
H. Jouin ◽  
S.J.P. Gobin ◽  
...  
Keyword(s):  
Human Chromosome ◽  
Interferon Γ ◽  
Chromosome 21 ◽  
Human Interferon ◽  
Human Chromosome 21
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