scholarly journals Analysis of structural determinants of the stability of thermolysinlike proteases by molecular modelling and site-directed mutagenesis

1996 ◽  
Vol 9 (12) ◽  
pp. 1181-1189 ◽  
Author(s):  
Oene R. Veltman ◽  
Gert Vriend ◽  
Pieter J. Middelhoven ◽  
Bertus van den Burg ◽  
Gerard Venema ◽  
...  
Keyword(s):  
Molecular Modelling ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Structural Determinants ◽  
The Stability

Site-directed mutagenesis and homology modeling indicate an important role of cysteine 439 in the stability of betaine aldehyde dehydrogenase from Pseudomonas aeruginosa

Biochimie ◽  
2005 ◽  
Vol 87 (12) ◽  
pp. 1056-1064 ◽  
Author(s):  
Lilian González-Segura ◽  
Roberto Velasco-García ◽  
Enrique Rudiño-Piñera ◽  
Carlos Mújica-Jiménez ◽  
Rosario A. Muñoz-Clares
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Homology Modeling ◽  
Aldehyde Dehydrogenase ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Betaine Aldehyde Dehydrogenase ◽  
Betaine Aldehyde ◽  
The Stability

scholarly journals Insights into the reaction mechanism of Escherichia coli agmatinase by site-directed mutagenesis and molecular modelling

2002 ◽  
Vol 269 (22) ◽  
pp. 5522-5526 ◽  
Author(s):  
Mónica Salas ◽  
Rolando Rodríguez ◽  
Nelia López ◽  
Elena Uribe ◽  
Vasthi López ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Reaction Mechanism ◽  
Molecular Modelling ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis

scholarly journals SITE DIRECTED MUTAGENESIS OF HUMAN INTERLEUKIN-2 GENE TO INCREASE THE STABILITY OF THE GENE PRODUCT- A BIOINFORMATICS APPROACH

2009 ◽  
Vol 1 (2) ◽  
pp. 4-13 ◽  
Author(s):  
Ashwin Kumar Dakshinamurthi ◽  
◽  
Manthira Vasagam Chidambaram ◽  
Vivek Anand Manivel ◽  
Swaminathan Detchanamurthy
Keyword(s):  
Gene Product ◽  
Interleukin 2 ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
The Stability

scholarly journals Kinetic Studies on CphA Mutants Reveal the Role of the P158-P172 Loop in Activity versus Carbapenems

2016 ◽  
Vol 60 (5) ◽  
pp. 3123-3126 ◽  
Author(s):  
Carlo Bottoni ◽  
Mariagrazia Perilli ◽  
Francesca Marcoccia ◽  
Alessandra Piccirilli ◽  
Cristina Pellegrini ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Catalytic Efficiency ◽  
Kinetic Studies ◽  
Zinc Ion ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Wild Type ◽  
The Stability

ABSTRACTSite-directed mutagenesis of CphA indicated that prolines in the P158-P172 loop are essential for the stability and the catalytic activity of subclass B2 metallo-β-lactamases against carbapenems. The sequential substitution of proline led to a decrease of the catalytic efficiency of the variant compared to the wild-type (WT) enzyme but also to a higher affinity for the binding of the second zinc ion.

scholarly journals Aminopeptidase B, a glucagon-processing enzyme: site directed mutagenesis of the Zn2+-binding motif and molecular modelling

2007 ◽  
Vol 8 (1) ◽  
pp. 21 ◽  
Author(s):  
Viet-Lai Pham ◽  
Marie-Sandrine Cadel ◽  
Cecile Gouzy-Darmon ◽  
Chantal Hanquez ◽  
Margery C Beinfeld ◽  
...  
Keyword(s):  
Molecular Modelling ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Binding Motif ◽  
Processing Enzyme

scholarly journals The Role of the Disulfide Bridge in the Stability and Structural Integrity of Ovalbumin Evaluated by Site-Directed Mutagenesis

2011 ◽  
Vol 75 (3) ◽  
pp. 544-549 ◽  
Author(s):  
Takayuki ISHIMARU ◽  
Kazunari ITO ◽  
Miho TANAKA ◽  
Syunpei TANAKA ◽  
Naotoshi MATSUDOMI
Keyword(s):  
Structural Integrity ◽  
Disulfide Bridge ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
The Stability
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