scholarly journals Usability of donor corneas harvested from the deceased having septicaemia or malignancy

QJM ◽  
2019 ◽  
Vol 112 (9) ◽  
pp. 681-683 ◽  
Author(s):  
T Singh ◽  
S K Arya ◽  
U Handa ◽  
J Chander
Keyword(s):  
Cancer Patients ◽  
Histopathological Examination ◽  
Supply And Demand ◽  
Corneal Transplantation ◽  
Microbiological Analysis ◽  
Corneal Tissue ◽  
Cadaveric Donor ◽  
Wide Gap ◽  
Culture Positive ◽  
Donor Corneas

Abstract Context There is a wide gap between supply and demand in relation to healthy corneal grafts. Specific contraindications like infection and malignancy lead to non-usage of many grafts, despite the fact that deeming graft unhealthyness for these two contraindications is debatable. Aims This study was conceptualized to assess if corneas donated from the deceased with septicaemia or malignancy can be deemed fit for implantation. Settings and design Retrospective histopathological and microbiological analysis of cadaveric donor corneas. Methods A total of 76 donor corneas from 38 patients rejected for corneal transplantation in view of patient having septicaemia or malignancy were analysed for pathological and microbiological workup, to look for dissemination of disease within corneal tissue. Pathology workup included gross and microscopic histopathological evaluation of tissue. Microbiology workup included Grams stain and KOH with calcofluor mount, culture in blood agar, chocolate agar, Sabourauds dextrose agar and Mc Conkeys broth. Results A total of 46 donor corneas of 23 septicaemia patients when evaluated showed presence of culture positive infection in 18 patients (78.2%). Histopathological examination done for 30 donor corneas from 15 cancer patients did not reveal presence of tumour cells in the specimen. Corneas of two of cancer patients having septicaemia revealed growth on cultures. Conclusions Corneal tissues harvested from septicaemia donors showed significantly higher incidence of corneal contamination, confirming their unsuitability for usage. However, there was no incidence of tumour transmission in corneal tissues of the patients with malignancies, suggesting that they can be considered for ophthalmic purpose.

Human corneal endothelial cells grafts to replace cadaveric donor corneas

2021 ◽  
Author(s):  
Moataz Dowaidar
Keyword(s):  
Endothelial Cells ◽  
Culture Media ◽  
Corneal Transplantation ◽  
Ocular Tissue ◽  
Corneal Endothelial Cells ◽  
Cadaveric Donor ◽  
Human Corneal Endothelial Cells ◽  
The Future ◽  
Limbal Stem Cell Transplantation ◽  
Donor Corneas

Human corneal endothelial cells (HCEC) grafts to replace cadaveric donor corneas have made tremendous progress in recent years, and this article highlights the most recent discoveries and important achievements in this area. Cell injection therapy with cultured HCEC has just finished its first clinical research, which showed promising results. This follows on the heels of the success of autologous limbal stem cell transplantation in bioengineered ocular tissue transplantation. Transplantation techniques like bioengineered endothelium sheet transplantation and full-thickness corneal transplantation are likely to become more prevalent in the future decade. This goal will be achieved by a combination of current advancements in HCEC propagation, high-quality culture media, and 3D or 4D tissue culture, which will be the trend in the future.

scholarly journals Regenerative capacity of the corneal transition zone for endothelial cell therapy

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Nicole Ming Sie ◽  
Gary Hin-Fai Yam ◽  
Yu Qiang Soh ◽  
Matthew Lovatt ◽  
Deepinder Dhaliwal ◽  
...  
Keyword(s):  
Transition Zone ◽  
Corneal Endothelium ◽  
Ex Vivo ◽  
Corneal Transplantation ◽  
Corneal Stroma ◽  
Regenerative Capacity ◽  
In Vivo Models ◽  
Endothelial Progenitors ◽  
Donor Corneas

AbstractThe corneal endothelium located on the posterior corneal surface is responsible for regulating stromal hydration. This is contributed by a monolayer of corneal endothelial cells (CECs), which are metabolically active in a continuous fluid-coupled efflux of ions from the corneal stroma into the aqueous humor, preventing stromal over-hydration and preserving the orderly arrangement of stromal collagen fibrils, which is essential for corneal transparency. Mature CECs do not have regenerative capacity and cell loss due to aging and diseases results in irreversible stromal edema and a loss of corneal clarity. The current gold standard of treatment for this worldwide blindness caused by corneal endothelial failure is the corneal transplantation using cadaveric donor corneas. The top indication is Fuchs corneal endothelial dystrophy/degeneration, which represents 39% of all corneal transplants performed. However, the global shortage of transplantable donor corneas has restricted the treatment outcomes, hence instigating a need to research for alternative therapies. One such avenue is the CEC regeneration from endothelial progenitors, which have been identified in the peripheral endothelium and the adjacent transition zone. This review examines the evidence supporting the existence of endothelial progenitors in the posterior limbus and summarizes the existing knowledge on the microanatomy of the transitional zone. We give an overview of the isolation and ex vivo propagation of human endothelial progenitors in the transition zone, and their growth and differentiation capacity to the corneal endothelium. Transplanting these bioengineered constructs into in vivo models of corneal endothelial degeneration will prove the efficacy and viability, and the long-term maintenance of functional endothelium. This will develop a novel regenerative therapy for the management of corneal endothelial diseases.

PORTABLE KERATOMETRY OF CADAVERIC DONOR CORNEAS

Cornea ◽  
1994 ◽  
Vol 13 (1) ◽  
pp. 102
Author(s):  
James P. McCulley ◽  
Ajay S. Dave
Keyword(s):  
Cadaveric Donor ◽  
Donor Corneas

Demonstration of Feasibility of Application of a Portable Keratometer to Cadaveric Donor Corneas

Cornea ◽  
1994 ◽  
Vol 13 (5) ◽  
pp. 379-382 ◽  
Author(s):  
Ajay S. Dave ◽  
James P. McCulley
Keyword(s):  
Cadaveric Donor ◽  
Donor Corneas

scholarly journals Cancer Epidemiology Based on Hospital-Based Cancer Registry at National Referral Hospital of Indonesia, 2013

2021 ◽  
Vol 9 (1) ◽  
pp. 36
Author(s):  
Soehartati Gondhowiardjo
Keyword(s):  
Cancer Patients ◽  
Cancer Registry ◽  
Histopathological Examination ◽  
Lymphoblastic Leukemia ◽  
Locally Advanced ◽  
Nasopharyngeal Cancer ◽  
Epidemiological Data ◽  
Hospital Policy ◽  
Duct Carcinoma ◽  
Male Patients

Cancer is the primary cause of death in developed or developing country. To develop hospital policy and research, comprehensive epidemiological data are needed. This research aims to provide epidemiological and demographical profiles of cancer from a hospital-based cancer registry (HBCR) at Cipto Mangunkusumo Hospital in 2013. This is a descriptive study involving cancer patients based on the HBCR from January-December 2013. Cancer frequency at the hospital in 2013 was 4,915 cases, with the majority of the patients were 45-54 years old, regardless of their sexes. Sex ratio was 3:2 for women and men, respectively. The most frequent age of female patients was 45-54 years old, while for male patients was 55-64 years old. In 2013, the hospital served more cancer patients from outside Jakarta than from Jakarta itself. Breast cancer, cervical cancer, and hematopoietic and reticuloendothelial systems cancer were the most prevalent types of cancer in women. Additionally, hematopoietic and reticuloendothelial systems cancer, nasopharyngeal cancer, and lymphoma were the most prevalent types of cancer in men. Most breast and cervical cancers came in either locally advanced or advanced stage. Histopathological examination revealed that most prevalent types of cancer were Infiltrating duct carcinoma of breast, nonkeratinized squamous cell carcinoma of cervix, and acute lymphoblastic leukemia. Keywords: hospital-based cancer registry, epidemiology, histopathology, staging, demography.   Epidemiologi Kanker Berdasarkan Registrasi Kanker Berbasis Rumah Sakit di Pusat Rujukan Nasional Indonesia, 2013 Abstrak Kanker merupakan penyebab kematian utama pada negara maju maupun berkembang. Untuk menentukan arah kebijakan rumah sakit dan penelitian, diperlukan data komprehensif mengenai epidemiologi kanker. Penelitian ini bertujuan untuk memberikan gambaran profil epidemiologi dan demografi kanker di Rumah Sakit Cipto Mangunkusumo berdasarkan Registrasi Kanker Berbasis Rumah Sakit (HBCR) tahun 2013. Studi ini menggunakan desain deskriptif yang melibatkan semua pasien kanker berdasarkan data HBCR. pada Januari-Desember 2013. Frekuensi kanker di RSCM pada tahun 2013 adalah 4,915 kasus, dengan mayoritas pasien berusia 45-54 tahun, baik jenis kelamin wanita ataupun pria. Rasio jenis kelamin perempuan dibandingkan laki-laki adalah 3:2. Rentang usia paling sering pada pasien wanita adalah 45-54 tahun, sedangkan pada laki-laki adalah 55-64 tahun. Pada tahun 2013, RSCM lebih banyak melayani pasien kanker dari luar Jakarta dibandingkan dari Jakarta. Kanker payudara,serviks dan kanker darah dan sistem retikuloendotel merupakan jenis kanker tersering pada perempuan. Sebagai tambahan, kanker darah dan sistem retikuloendotel, nasofaring dan kegasanan kelenjar getah bening (limfoma) merupakan kanker tersering pada laki-laki. Sebagian besar kanker payudara dan serviks datang dengan stadium lokal lanjut atau lanjut. Pemeriksaan histopatologi menunjukkan bahwa tipe patologi kanker tersering adalah karsinoma duktal invasif pada kanker payudara, karsinoma sel skuamosa tidak berkeratin pada kanker serviks, dan leukemia limfoblastik akut. Kata kunci: Registrasi kanker berbasis rumah sakit, epidemiologi, histopatologi, stadium, demografi.

scholarly journals Total Corneal Transplantation after Trauma

2017 ◽  
Vol 17 (2) ◽  
pp. 37-38
Author(s):  
Elina Silina ◽  
Guna Laganovska
Keyword(s):  
Healthy Donor ◽  
Penetrating Keratoplasty ◽  
Ocular Trauma ◽  
Corneal Transplantation ◽  
Visual Outcome ◽  
Corneal Tissue ◽  
Full Thickness ◽  
Documented Case ◽  
Donor Graft ◽  
Penetrating Ocular Trauma

Abstract The first successful corneal transplantation is known since 1905, performed by Eduard Zirm (2). It has been implemented in order to restore vision in a variety of corneal diseases and after ocular traumas. The traditional technique for corneal transplantation, penetrating keratoplasty (PKP), refers to the full-thickness replacement of corneal tissue with a healthy donor graft (1). Authors report a well-documented case about successfully transplanted cornea after penetrating ocular trauma to improve visual outcome.

scholarly journals Cornea Regeneration as an Alternative to Human Donor Transplantation

2015 ◽  
Vol 09 (02) ◽  
pp. 111
Author(s):  
Isabelle Brunette ◽  
◽  
◽  
◽  
Emilio I Alarcon ◽  
...  
Keyword(s):  
Clinical Trials ◽  
Short Review ◽  
Corneal Tissue ◽  
Human Donor ◽  
Self Assembled ◽  
Donor Corneas

There is a need for an alternative to human donor corneas as the availability of good-quality tissues remains limited, with this situation potentially worsening as the population in many countries is progressively ageing. There have been numerous attempts to develop corneal equivalent as alternatives to donated human corneas as well as prostheses. In this short review, we focus on the efforts in bioengineering implants that promote regeneration by Canadian researchers, including our current team of authors. The examples of technologies developed that we describe include biomaterials that allow for partial regeneration of corneal tissue, self-assembled cornea constructs and cell-free corneal implants that promoted regeneration when evaluated in clinical trials in Europe.

scholarly journals Efficacy of adjuvant intrastromal and combination of intrastromal and intracameral voriconazole in Aspergillus fumigatus-induced moderate fungal keratitis in rabbits

2021 ◽  
Vol 30 (1) ◽  
pp. 13-9
Author(s):  
Dyah Tjintya Sarika ◽  
Melva Louisa ◽  
Anna Rozaliyani ◽  
Evelina ◽  
Made Susiyanti
Keyword(s):  
Aspergillus Fumigatus ◽  
Clinical Response ◽  
Histopathological Examination ◽  
Inflammatory Cells ◽  
Fungal Keratitis ◽  
Control Group ◽  
Corneal Tissue ◽  
Epithelial Defect ◽  
Treatment Groups

BACKGROUND There is no in vivo evidence for the effectiveness of adjuvant intrastromal and combination of intrastromal and intracameral voriconazole (VCZ) for treating Aspergillus fumigatus keratitis. This study aimed to compare the efficacy of both agents against it.  METHODS A randomized, masked, controlled experimental study was conducted on 11 albino New Zealand white rabbits in which moderate fungal keratitis was induced by inoculating spores of A. fumigatus to the cornea. The rabbits were allocated into 3 groups: 50 μg/0.1 ml intrastromal VCZ injection, 50 μg/0.1 ml intrastromal VCZ and intracameral VCZ injections, and topical VCZ (control). The treatment was given 5 days after inoculation. Epithelial defect, infiltrate size, corneal ulcer depth, and hypopyon were evaluated clinically. Histopathological and mycological examinations were also done 14 days after treatment.  RESULTS All rabbits in the adjuvant treatment groups demonstrated a tendency of a better clinical response with decreasing size of epithelial defect (p = 0.679) and infiltrate (p = 0.755) than in the control group. Direct microscopy, corneal culture, and chop corneal tissue culture were still positive in most of the rabbits from all groups. Histopathological examination showed an increase of inflammatory cells after treatment in all groups, especially in rabbits which were inoculated with A. fumigatus spores in both eyes.  CONCLUSIONS An adjuvant combination of intrastromal and intracameral VCZ showed a tendency of better clinical response for A. fumigatus-induced moderate fungal keratitis in rabbits. 

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