Effects of 1,25-Dihydroxyvitamin D3 on Interleukin-1 Production by the Human Monocyte Cell Line U937 and Peripheral Blood Monocytes

Rheumatology ◽  
1985 ◽  
Vol XXIV (suppl 1) ◽  
pp. 143-146 ◽  
Author(s):  
A.K. Bhalla ◽  
E.P. Amento ◽  
S.M. Krane
Keyword(s):  
Cell Line ◽  
Peripheral Blood ◽  
Interleukin 1 ◽  
Human Monocyte ◽  
Blood Monocytes ◽  
Peripheral Blood Monocytes ◽  
Dihydroxyvitamin D3 ◽  
1,25 Dihydroxyvitamin D3 ◽  
Human Monocyte Cell Line ◽  
Monocyte Cell

Effect of 1α,25-dihydroxyvitamin D3 on polyamine metabolism in human monocyte cell line-U937

1989 ◽  
Vol 21 (11) ◽  
pp. 1287-1294 ◽  
Author(s):  
Yukioka Kazuhiko ◽  
Otani Shuzo ◽  
Matsui-Yuasa Isao ◽  
Goto Hitoshi ◽  
Tahara Hideki ◽  
...  
Keyword(s):  
Cell Line ◽  
Human Monocyte ◽  
Polyamine Metabolism ◽  
Dihydroxyvitamin D3 ◽  
Human Monocyte Cell Line ◽  
Monocyte Cell

scholarly journals Isolation of the receptor for IgG from a human monocyte cell line (U937) and from human peripheral blood monocytes.

1982 ◽  
Vol 156 (6) ◽  
pp. 1794-1806 ◽  
Author(s):  
C L Anderson
Keyword(s):  
Cell Line ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Sodium Dodecyl ◽  
Fc Receptors ◽  
Human Monocyte ◽  
Fc Receptor ◽  
Soluble Form ◽  
Blood Monocytes ◽  
Peripheral Blood Monocytes

The Fc receptors for IgG from a human monocyte line (U937) and from highly purified human peripheral blood monocytes were solubilized, purified, and partially characterized. Both sources of cells gave indistinguishable results. Two molecules (or sets of molecules), one of about 72,000 mol wt and the other of 40,000-43,000 mol wt were discerned on autoradiograms of sodium dodecyl sulfate (SDS)-polyacrylamide gels analyzing acid eluates from Sepharose-IgG columns over which detergent lysates of radioiodinated cells had been passed. The larger of the two molecules, p72, accounted for greater than or equal to 90% of the radioactivity. This component was noted to be heterodisperse both by size on SDS gels and by charge on isoelectric focusing gels. The charge heterogeneity, being virtually eliminated by neuraminidase and tunicamycin, was probably due to variable glycosylation. Several lines of evidence indicated that p72 is probably all or part of the Fc receptor: (a) radiolabeling of this molecule using chloroglycouril was blocked by IgG of the Fc receptor; (b) in soluble form this molecule expressed ligand specificity identical to the in situ receptor; (c) the molecule was not recovered from affinity adsorbants bearing proteins that do not bind to the Fc receptors, nor (d) from a human T cell line that does not bear Fc receptors. The smaller of the two molecules isolated, p40-43, is at least in part actin. Its relationship to p72 is not understood.

Human monocyte cell line (U937) releases suppressive IgG-binding factor(s)

1986 ◽  
Vol 16 (1) ◽  
pp. 25-30 ◽  
Author(s):  
Charles Félix Calvo ◽  
Shinichiro Watanabe ◽  
Didier Mètivier ◽  
Anna Senik
Keyword(s):  
Cell Line ◽  
Human Monocyte ◽  
Igg Binding ◽  
Binding Factor ◽  
Human Monocyte Cell Line ◽  
Monocyte Cell

Is the Success of Cefazolin plus Ertapenem in Methicillin-Susceptible Staphylococcus aureus Bacteremia Based on Release of Interleukin 1-beta?

2022 ◽  
Author(s):  
Dan Smelter ◽  
Mary Hayney ◽  
George Sakoulas ◽  
Warren Rose
Keyword(s):  
Staphylococcus Aureus ◽  
Peripheral Blood ◽  
Interleukin 1 ◽  
Interleukin 1 Beta ◽  
Blood Monocytes ◽  
Salvage Regimen ◽  
Peripheral Blood Monocytes ◽  
Staphylococcus Aureus Bacteremia

Cefazolin and ertapenem has been shown to be an effective salvage regimen for refractory methicillin-susceptible Staphylococcus aureus bacteremia. Our findings suggest cefazolin plus ertapenem in vitro stimulates interleukin-1β release from peripheral blood monocytes both with and without S. aureus presence. This IL-1β augmentation was primarily driven by ertapenem. These findings support further exploration of cefazolin plus ertapenem in MSSA bacteremia and may partially explain its marked potency in vivo despite modest synergy in vitro .

scholarly journals Induction of human monocyte to macrophage maturation in vitro by 1,25- dihydroxyvitamin D3

Blood ◽  
1990 ◽  
Vol 76 (12) ◽  
pp. 2457-2461 ◽  
Author(s):  
M Kreutz ◽  
R Andreesen
Keyword(s):  
Serum Proteins ◽  
Primary Cultures ◽  
Human Monocyte ◽  
Mononuclear Phagocyte ◽  
Mononuclear Phagocyte System ◽  
Blood Monocytes ◽  
Necrosis Factor Alpha ◽  
Dihydroxyvitamin D3 ◽  
1,25 Dihydroxyvitamin D3

Abstract Cells of the mononuclear phagocyte system arise from circulating blood monocytes (MO) that undergo further maturation on leaving the vasculature and migration into the various tissues and body cavities. This terminal differentiation step is also observed in vitro when blood MO are cultured in the presence of serum. Yet, the inducing signals present in serum are not defined. We have established primary cultures from elutriation-purified blood MO and found that the active metabolite of vitamin D3 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) could induce maturation of MO to macrophages (MAC) in the absence of any serum proteins. Cells were cultured for 7 days with AB-group serum or 1,25(OH)2D3, respectively, and MO maturation analyzed by morphology, functional activity, and the expression of lineage-restricted maturation-associated antigens (MAX.1, MAX.3). At an optimal concentration of 10(-8) mol/L, 1,25(OH)2D3 promoted the development of fully differentiated MAC whose phenotype and functional competence in terms of cytokine release (tumor necrosis factor alpha, interleukin-6, fibronectin, and lysozyme) was comparable with MAC grown in serum. In conclusion, our data may add to the immunoregulatory potential of 1,25(OH)2D3, which may play an essential role in the ontogeny of the mononuclear phagocyte system.

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