Separation and Characterization of Barley Starch Polymers by a Flow Field-Flow Fractionation Technique in Combination with Multiangle Light Scattering and Differential Refractive Index Detection

2002 ◽  
Vol 79 (5) ◽  
pp. 624-630 ◽  
Author(s):  
S. You ◽  
S. G. Stevenson ◽  
M. S. Izydorczyk ◽  
K. R. Preston
Keyword(s):  
Refractive Index ◽  
Light Scattering ◽  
Flow Field ◽  
Field Flow Fractionation ◽  
Flow Fractionation ◽  
Refractive Index Detection

scholarly journals Fast and Purification-Free Characterization of Bio-Nanoparticles in Biological Media by Electrical Asymmetrical Flow Field-Flow Fractionation Hyphenated with Multi-Angle Light Scattering and Nanoparticle Tracking Analysis Detection

Molecules ◽  
2020 ◽  
Vol 25 (20) ◽  
pp. 4703
Author(s):  
Roland Drexel ◽  
Agnieszka Siupa ◽  
Pauline Carnell-Morris ◽  
Michele Carboni ◽  
Jo Sullivan ◽  
...  
Keyword(s):  
Light Scattering ◽  
Flow Field ◽  
Chemical Characterization ◽  
Rabbit Serum ◽  
Field Flow Fractionation ◽  
Nanoparticle Tracking Analysis ◽  
Biological Media ◽  
Physico Chemical ◽  
Flow Fractionation

Accurate physico-chemical characterization of exosomes and liposomes in biological media is challenging due to the inherent complexity of the sample matrix. An appropriate purification step can significantly reduce matrix interferences, and thus facilitate analysis of such demanding samples. Electrical Asymmetrical Flow Field-Flow Fractionation (EAF4) provides online sample purification while simultaneously enabling access to size and Zeta potential of sample constituents in the size range of approx. 1–1000 nm. Hyphenation of EAF4 with Multi-Angle Light Scattering (MALS) and Nanoparticle Tracking Analysis (NTA) detection adds high resolution size and number concentration information turning this setup into a powerful analytical platform for the comprehensive physico-chemical characterization of such challenging samples. We here present EAF4-MALS hyphenated with NTA for the analysis of liposomes and exosomes in complex, biological media. Coupling of the two systems was realized using a flow splitter to deliver the sample at an appropriate flow speed for the NTA measurement. After a proof-of-concept study using polystyrene nanoparticles, the combined setup was successfully applied to analyze liposomes and exosomes spiked into cell culture medium and rabbit serum, respectively. Obtained results highlight the benefits of the EAF4-MALS-NTA platform to study the behavior of these promising drug delivery vesicles under in vivo like conditions.

Sign in / Sign up

Export Citation Format

Share Document