Исследование рефрактометром дифференциального типа влияния оптической плотности текущей жидкости на погрешность измерения показателя преломления

Factors that are affected the influence of the measurement error of the refractive index n in the flowing liquid (change in its optical density and temperature) are established. A during the using a differential flow type refractometer for measuring n flowing fliquid on the error in measuring the refractive index is additionally influenced by the scattering of radiation in the cell transducer and the wedge-shaped glass of the cell. New design of a differential refractometer for investigation of the optical density influence of the flowing liquid on the measurement error n is developped. New results confirming the influence of changes in the optical density of the flowing liquid on the measuring error of the refractive index n are recived. Compensation technique of the changes influence a optical density on the measurement error n is offered.

Determination of Sugars in β-Amylase Hydrolysates by High Performance Liquid Chromatography

The chromatographic conditions for the separation of glucose, maltose and maltotriose on a ZORBAX Eclipse XDB-C18 column (4 um, 4.6×250mm) by use of a 2414 differential refractometer detector were determined. The square of the correlation coefficient of glucose, sucrose, maltose and maltotriose was 0.9973, 0.9981, 0.9977 and 0.9987, respectively. When the ratio of signal and noise was 3, the detection limit of glucose, sucrose, maltose and maltotriose was 0.3, 0.3, 0.20, 0.20 mg/mL the β-amylase hydrolysates consisted mainly of maltose, and maltotriose next, glucose only in trace amount, and no sucrose. The RSD of glucose, maltose and maltotriose was 2.08%, 2.41% and 2.61%, respectively. And the recovery of glucose, maltose and maltotriose was 101.3%, 98.4% and 93.5%. It was credible to separate sugars from hydrolysates. The method is important for improving the inducible enzymes activity.

Requirements on a Differential Refractometer for its Use in Sizing Colloidal Particles

Most colloidal samples have a non-negligible imaginary component of the effective refractive index due to scattering and absorption losses. In this paper we analyze the requirements on a differential refractometer so that it can be used to measure the real part of the effective refractive index increment in colloids for particle size analysis.