scholarly journals Genome-Wide Association of Rice Blast Disease Resistance and Yield-Related Components of Rice

2015 ◽  
Vol 28 (12) ◽  
pp. 1383-1392 ◽  
Author(s):  
Xueyan Wang ◽  
Melissa H. Jia ◽  
Pooja Ghai ◽  
Fleet N. Lee ◽  
Yulin Jia
Keyword(s):  
Disease Resistance ◽  
Plant Height ◽  
Ssr Markers ◽  
Rice Blast ◽  
Seed Weight ◽  
Blast Resistance ◽  
Heading Date ◽  
Rice Blast Disease ◽  
Blast Disease ◽  
Yield Potential

Robust disease resistance may require an expenditure of energy that may limit crop yield potential. In the present study, a subset of a United States Department of Agriculture rice core collection consisting of 151 accessions was selected using a major blast resistance (R) gene, Pi-ta, marker and was genotyped with 156 simple sequence repeat (SSR) markers. Disease reactions to Magnaporthe oryzae, the causal agent of rice blast disease, were evaluated under greenhouse and field conditions, and heading date, plant height, paddy and brown seed weight in two field environments were analyzed, using an association mapping approach. A total of 21 SSR markers distributed among rice chromosomes 2 to 12 were associated with blast resistance, and 16 SSR markers were associated with seed weight, heading date, and plant height. Most noticeably, shorter plants were significantly correlated with resistance to blast, rice genomes with Pi-ta were associated with lighter seed weights, and the susceptible alleles of RM171 and RM6544 were associated with heavier seed weight. These findings unraveled a complex relationship between disease resistance and yield-related components.

scholarly journals Cloning and Function Analysis of a Novel Rice Blast Resistance Gene Pi65 in Japonica Rice

2021 ◽  
Author(s):  
Lili Wang ◽  
Zuobin Ma ◽  
Houxiang Kang ◽  
Shuang Gu ◽  
Zhanna Mukhina ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Resistance Gene ◽  
Rice Blast ◽  
Blast Resistance ◽  
Rice Blast Disease ◽  
Blast Disease ◽  
Resistant Variety ◽  
Japonica Rice ◽  
Rice Blast Resistance ◽  
Blast Resistance Gene

Abstract Rice blast seriously threatens rice production worldwide. Utilizing the rice blast resistance gene to breed the rice blast resistant varieties is one of the best ways to control rice blast disease. Using a map-based cloning strategy, here, we cloned a novel rice blast resistance gene, Pi65 from the resistant variety GangYu129 (abbreviated GY129, O. sativa japonica ). Overexpression of Pi65 in the susceptible variety LiaoXing1 (abbreviated LX1, O. sativa japonica ) enhanced rice blast resistance, while knockout of Pi65 in GY129 resulted in susceptible to rice blast disease. Pi65 encodes two transmembrane domains, with 15 LRR domains and one serine/threonine protein kinase catalytic domain, conferring resistance to isolates of M. oryzae collected from northeast China. There are sixteen amino acids differences between the Pi65 resistance and susceptible alleles. Compared with the Pi65 resistant allele, the susceptible allele deleted one LRR domain. Pi65 was constitutively expressed in whole plants, and it could be induce expressed in the early stage of M. oryzae infection . Transcriptome analysis revealed that numerous genes associated with disease resistance were specifically upregulated in GY129 24-hour post inoculation (HPI), on the contrary, the photosynthesis-and carbohydrate metabolism-related genes were particularly downregulated 24 HPI, demonstrating that the disease resistance associated genes has been activated in GY129 (carrying Pi65 ) after rice blast fungal infection, and the cellular basal and energy metabolism was inhibited simultaneously. Our study provides genetic resources for improving rice blast resistance as well as enriches the study of rice blast resistance mechanisms.

scholarly journals Level of Endogenous Jasmonate is Critical for Durable Broad-Spectrum Disease Resistance Against Rice Blast in a Japonica Rice Variety, Ziyu44

2021 ◽  
Author(s):  
Xingyu An ◽  
Hui Zhang ◽  
Jinlu Li ◽  
Rui Yang ◽  
Qianchun Zeng ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Signaling Pathway ◽  
Broad Spectrum ◽  
Rice Blast ◽  
Blast Resistance ◽  
Rice Variety ◽  
Blast Disease ◽  
Japonica Rice ◽  
Mycelium Growth ◽  
Broad Spectrum Resistance

Abstract Background: The molecular mechanism of durable and broad-spectrum resistance to rice blast disease in japonica rice variety is still very little known. Ziyu44, a local japonica rice variety in Yunnan Province of China, has shown durable broad-spectrum blast resistance for more than 30 years, and provides an opportunity for us to explore the molecular basis of broad-spectrum resistance to rice blast in japonica rice variety.Methods and Results: We conducted a comparative study of mycelium growth, aposporium formation, the accumulation of salicylate(SA), jasmonate(JA) and H2O2, the expression of SA- and JA-associated genes between Ziyu44 and susceptible variety Jiangnanxiangnuo (JNXN) upon M. oryzae infection. We found that appressorium formation and invasive hyphae extention were greatly inhibited in Ziyu 44 leaves compared with that in JNXN leaves. Both Ziyu 44 and JNXN plants maintained high levels of baseline SA and did not show increased accumulation of SA after inoculation with M. oryzae, while the levels of baseline JA in Ziyu 44 and JNXN plants were relatively low, and the accumulation of JA exhibited markedly increased in Ziyu 44 plants upon M. oryzae infection. The expression levels of key genes involving JA and SA signaling pathway OsCOI1b, OsNPR1, OsMPK6 as well as pathogenesis-related (PR) genes OsPR1a, OsPR1b and OsPBZ1, were markedly up-regulated in Ziyu44. Conclusions: The level of endogenous JA is critical for synchronous activation of SA and JA signaling pathway, up-regulating PR gene expression and enhancing disease resistance against rice blast in Ziyu44.

Investigating the biology of rice blast disease and prospects for durable resistance

2021 ◽  
pp. 643-680
Author(s):  
Vincent M. Were ◽  
◽  
Nicholas J. Talbot ◽  
Keyword(s):  
Cell Biology ◽  
Rice Blast ◽  
Plant Pathogens ◽  
Blast Resistance ◽  
Durable Resistance ◽  
Rice Blast Disease ◽  
Blast Disease ◽  
Global Food Security ◽  
Major Resistance Genes ◽  
Effector Secretion

There are important biological process involved in rice blast disease that are now well-studied during the early events in plant infection which include: the cell biology of appressorium formation, the biology of invasive growth and effector secretion, the two distinct mechanisms of effector secretion, the nature of the plant-pathogen interface, PAMP-triggered immunity modulation by secreted effectors and effector-triggered immunity and blast resistance. The devastating losses caused by the blast fungus have been documented in most grasses, but this chapter discusses the use of major resistance genes to rice blast and wheat blast disease as an emerging threat to global food security. This chapter also highlights an emerging approach to breed for durable resistance to plant pathogens using gene editing technologies with an example: CRISPR-Cas9 mutagenesis of dominant S-genes for disease control.

Development and validation of functional marker targeting an InDel in the major rice blast disease resistance gene Pi54 (Pik h )

2010 ◽  
Vol 27 (1) ◽  
pp. 129-135 ◽  
Author(s):  
G. Ramkumar ◽  
K. Srinivasarao ◽  
K. Madhan Mohan ◽  
I. Sudarshan ◽  
A. K. P. Sivaranjani ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Resistance Gene ◽  
Rice Blast ◽  
Rice Blast Disease ◽  
Functional Marker ◽  
Blast Disease ◽  
Disease Resistance Gene ◽  
Development And Validation

scholarly journals Integrated strategies for durable rice blast resistance in sub-Saharan Africa

Plant Disease ◽  
2021 ◽  
Author(s):  
Samuel K Mutiga ◽  
Felix Rotich ◽  
Vincent M Were ◽  
John Kimani ◽  
David Thuranira Mwongera ◽  
...  
Keyword(s):  
Rice Blast ◽  
Crop Production ◽  
Blast Resistance ◽  
Research Effort ◽  
Rice Production ◽  
Rice Blast Disease ◽  
Sub Saharan Africa ◽  
Blast Disease ◽  
Small Scale ◽  
Sub Saharan

Rice is a key food security crop in Africa. The importance of rice has led to increasing country-specific, regional and multinational efforts to develop germplasm and policy initiatives to boost production for a more food secure continent. Currently, this critically important cereal crop is predominantly cultivated by small-scale farmers under sub-optimal conditions in most parts of sub-Saharan Africa (SSA). Rice blast disease, caused by the fungus Magnaporthe oryzae, represents one of the major biotic constraints to rice production under small-scale farming systems of Africa, and developing durable disease resistance is therefore of critical importance. In this review, we provide an overview of the major advances by a multinational collaborative research effort to enhance sustainable rice production across SSA and how it is affected by advances in regional policy. As part of the multinational effort, we highlight the importance of joint international partnerships in tackling multiple crop production constraints through integrated research and outreach programs. More specifically, we highlight recent progress in establishing international networks for rice blast disease surveillance, farmer engagement, monitoring pathogen virulence spectra, and the establishment of regionally-based blast resistance breeding programs. To develop blast resistant, high yielding rice varieties for Africa, we have established a breeding pipeline that utilizes real-time data of pathogen diversity and virulence spectra, to identify major and minor blast resistance genes for introgression into locally adapted rice cultivars. In addition, the project has developed a package to support sustainable rice production through regular stakeholder engagement, training of agricultural extension officers, and establishment of plant clinics.

Recent Insights in Rice Blast Disease Resistance

2021 ◽  
pp. 89-123
Author(s):  
Susheel Kumar Sharma ◽  
Devender Sharma ◽  
Ram Prasnna Meena ◽  
Manoj Kumar Yadav ◽  
H. Rajashekara ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Rice Blast ◽  
Rice Blast Disease ◽  
Blast Disease

Confirming and Identifying New Loci for Rice Blast Disease Resistance using Magnaporthe oryzae Field Isolates in the US

Crop Science ◽  
2015 ◽  
Vol 55 (6) ◽  
pp. 2620-2627 ◽  
Author(s):  
Junjie Xing ◽  
Melissa H Jia ◽  
James C. Correll ◽  
Longping Yuan ◽  
Huangfeng Deng ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Magnaporthe Oryzae ◽  
Rice Blast ◽  
Rice Blast Disease ◽  
Blast Disease ◽  
Field Isolates ◽  
The Us

scholarly journals Osa‐miR398b boosts H 2 O 2 production and rice blast disease‐resistance via multiple superoxide dismutases

2019 ◽  
Vol 222 (3) ◽  
pp. 1507-1522 ◽  
Author(s):  
Yan Li ◽  
Xiao‐Long Cao ◽  
Yong Zhu ◽  
Xue‐Mei Yang ◽  
Kai‐Ni Zhang ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Rice Blast ◽  
Rice Blast Disease ◽  
Blast Disease ◽  
Superoxide Dismutases

A novel wall-associated receptor-like protein kinase gene, OsWAK1, plays important roles in rice blast disease resistance

2008 ◽  
Vol 69 (3) ◽  
pp. 337-346 ◽  
Author(s):  
Hui Li ◽  
Shan-Yue Zhou ◽  
Wen-Sheng Zhao ◽  
Sheng-Chang Su ◽  
You-Liang Peng
Keyword(s):  
Disease Resistance ◽  
Protein Kinase ◽  
Rice Blast ◽  
Rice Blast Disease ◽  
Blast Disease ◽  
Kinase Gene ◽  
Protein Kinase Gene
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