scholarly journals OsLSD1, a Rice Zinc Finger Protein, Regulates Programmed Cell Death and Callus Differentiation

2005 ◽  
Vol 18 (5) ◽  
pp. 375-384 ◽  
Author(s):  
Lijuan Wang ◽  
Zhongyou Pei ◽  
Yingchuan Tian ◽  
Chaozu He
Keyword(s):  
Cell Death ◽  
Programmed Cell Death ◽  
Zinc Finger ◽  
Transgenic Rice ◽  
Zinc Finger Protein ◽  
Protein Fusion ◽  
Rice Plants ◽  
Blast Fungus ◽  
Transgenic Rice Plants ◽  
Callus Differentiation

The Arabidopsis LSD1 and LOL1 proteins both contain three conserved zinc finger domains and have antagonistic effects on plant programmed cell death (PCD). In this study, a rice (Oryza sativa) functional homolog of LSD1, designated OsLSD1, was identified. The expression of OsLSD1 was light-induced or dark-suppressed. Overexpression of OsLSD1 driven by the cauliflower mosaic virus 35S promoter accelerated callus differentiation in transformed rice tissues and increased chlorophyll b content in transgenic rice plants. Antisense transgenic rice plants exhibited lesion mimic phenotype, increased expression of PR-1mRNA, and an accelerated hypersensitive response when inoculated with avirulent isolates of blast fungus. Both sense and antisense transgenic rice plants conferred significantly enhanced resistance against a virulent isolate of blast fungus. Moreover, ectopic overexpression of OsLSD1 in transgenic tobacco (Nicotiana tabacum) enhanced the tolerance to fumonisins B1 (FB1), a PCD-eliciting toxin. OsLSD1 green fluorescent protein fusion protein was located in the nucleus of tobacco cells. Our results suggest that OsLSD1 plays a negative role in regulating plant PCD, whereas it plays a positive role in callus differentiation.

Zinc finger protein RP-8, the Bombyx mori ortholog of programmed cell death 2, regulates cell proliferation

2020 ◽  
Vol 104 ◽  
pp. 103542 ◽  
Author(s):  
Muhammad Nadeem Abbas ◽  
Hanghua Liang ◽  
Saima Kausar ◽  
Zhen Dong ◽  
Hongjuan Cui
Keyword(s):  
Cell Proliferation ◽  
Cell Death ◽  
Programmed Cell Death ◽  
Bombyx Mori ◽  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Finger Protein

scholarly journals Requirement for the Murine Zinc Finger Protein ZFR in Perigastrulation Growth and Survival

2001 ◽  
Vol 21 (8) ◽  
pp. 2880-2890 ◽  
Author(s):  
Madeleine J. Meagher ◽  
Robert E. Braun
Keyword(s):  
Cell Death ◽  
Programmed Cell Death ◽  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Cellular Growth ◽  
Gene Encoding ◽  
Growth And Survival ◽  
Finger Protein ◽  
Development Delay ◽  
Cellular Phenotypes

ABSTRACT The transition from preimplantation to postimplantation development leads to the initiation of complex cellular differentiation and morphogenetic movements, a dramatic decrease in cell cycle length, and a commensurate increase in the size of the embryo. Accompanying these changes is the need for the transfer of nutrients from the mother to the embryo and the elaboration of sophisticated genetic networks that monitor genomic integrity and the homeostatic control of cellular growth, differentiation, and programmed cell death. To determine the function of the murine zinc finger protein ZFR in these events, we generated mice carrying a null mutation in the gene encoding it. Homozygous mutant embryos form normal-appearing blastocysts that implant and initiate the process of gastrulation. Mutant embryos form mesoderm but they are delayed in their development and fail to form normal anterior embryonic structures. Loss of ZFR function leads to both an increase in programmed cell death and a decrease in mitotic index, especially in the region of the distal tip of the embryonic ectoderm. Mutant embryos also have an apparent reduction in apical vacuoles in the columnar visceral endoderm cells in the extraembryonic region. Together, these cellular phenotypes lead to a dramatic development delay and embryonic death by 8 to 9 days of gestation, which are independent of p53 function.

scholarly journals Down-regulation of OsSAG12-1 results in enhanced senescence and pathogen-induced cell death in transgenic rice plants

2013 ◽  
Vol 38 (3) ◽  
pp. 583-592 ◽  
Author(s):  
Subaran Singh ◽  
Mrunmay Kumar Giri ◽  
Praveen Kumar Singh ◽  
Adnan Siddiqui ◽  
Ashis Kumar Nandi
Keyword(s):  
Cell Death ◽  
Transgenic Rice ◽  
Down Regulation ◽  
Rice Plants ◽  
Transgenic Rice Plants

The zinc finger protein ZAT11 modulates paraquat-induced programmed cell death in Arabidopsis thaliana

2013 ◽  
Vol 35 (6) ◽  
pp. 1863-1871 ◽  
Author(s):  
Muhammad Kamran Qureshi ◽  
Neerakkal Sujeeth ◽  
Tsanko S. Gechev ◽  
Jacques Hille
Keyword(s):  
Arabidopsis Thaliana ◽  
Cell Death ◽  
Programmed Cell Death ◽  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Finger Protein

scholarly journals DPL-1 DP, LIN-35 Rb and EFL-1 E2F Act With the MCD-1 Zinc-Finger Protein to Promote Programmed Cell Death in Caenorhabditis elegans

Genetics ◽  
2007 ◽  
Vol 175 (4) ◽  
pp. 1719-1733 ◽  
Author(s):  
Peter W. Reddien ◽  
Erik C. Andersen ◽  
Michael C. Huang ◽  
H. Robert Horvitz
Keyword(s):  
Cell Death ◽  
Caenorhabditis Elegans ◽  
Programmed Cell Death ◽  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Finger Protein

scholarly journals OsNAC111, a Blast Disease–Responsive Transcription Factor in Rice, Positively Regulates the Expression of Defense-Related Genes

2014 ◽  
Vol 27 (10) ◽  
pp. 1027-1034 ◽  
Author(s):  
Naoki Yokotani ◽  
Tomoko Tsuchida-Mayama ◽  
Hiroaki Ichikawa ◽  
Nobutaka Mitsuda ◽  
Masaru Ohme-Takagi ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Transgenic Rice ◽  
Transcriptional Activation ◽  
Rice Blast ◽  
Rice Blast Fungus ◽  
Blast Disease ◽  
Pr Genes ◽  
Rice Plants ◽  
Blast Fungus ◽  
Transgenic Rice Plants

Plants respond to pathogen attack by transcriptionally regulating defense-related genes via various types of transcription factors. We identified a transcription factor in rice, OsNAC111, belonging to the TERN subgroup of the NAC family that was transcriptionally upregulated after rice blast fungus (Magnaporthe oryzae) inoculation. OsNAC111 was localized in the nucleus of rice cells and had transcriptional activation activity in yeast and rice cells. Transgenic rice plants overexpressing OsNAC111 showed increased resistance to the rice blast fungus. In OsNAC111-overexpressing plants, the expression of several defense-related genes, including pathogenesis-related (PR) genes, was constitutively high compared with the control. These genes all showed blast disease-responsive expression in leaves. Among them, two chitinase genes and one β-1,3-glucanase gene showed reduced expression in transgenic rice plants in which OsNAC111 function was suppressed by a chimeric repressor (OsNAC111-SRDX). OsNAC111 activated transcription from the promoters of the chitinase and β-1,3-glucanase genes in rice cells. In addition, brown pigmentation at the infection sites, a defense response of rice cells to the blast fungus, was lowered in OsNAC111-SRDX plants at the early infection stage. These results indicate that OsNAC111 positively regulates the expression of a specific set of PR genes in the disease response and contributes to disease resistance.

scholarly journals ZNF1 Encodes a Putative C2H2 Zinc-Finger Protein Essential for Appressorium Differentiation by the Rice Blast Fungus Magnaporthe oryzae

2016 ◽  
Vol 29 (1) ◽  
pp. 22-35 ◽  
Author(s):  
Xiaofeng Yue ◽  
Yawei Que ◽  
Lin Xu ◽  
Shuzhen Deng ◽  
Youliang Peng ◽  
...  
Keyword(s):  
Zinc Finger ◽  
Magnaporthe Oryzae ◽  
Rice Blast ◽  
Zinc Finger Protein ◽  
Rice Blast Fungus ◽  
Mitogen Activated Protein Kinase ◽  
Protein Fusion ◽  
C2h2 Zinc Finger ◽  
Blast Fungus ◽  
Appressorium Development

The rice blast fungus Magnaporthe oryzae forms specialized infection structures called appressoria which are essential for gaining entry to plant tissue. Here, we report the identification of a novel nonpathogenic T-DNA-tagged mutant XF696 of M. oryzae with a single insertion in the promoter of ZNF1, which encodes a putative transcription factor (TF). Targeted gene deletion mutants of ZNF1 are nonpathogenic and unable to develop appressoria. However, Δznf1 mutants still respond to exogenous cyclic AMP on hydrophilic surfaces and can sense hydrophobic surfaces, initiating the differentiation of germ tubes. Interestingly, Δznf1 mutants also produce significantly more conidia compared with the isogenic wild-type strain. Quantitative reverse-transcription polymerase chain reaction analysis and green fluorescent protein fusion experiments revealed that expression of ZNF1 was highly induced during germination and appressorium development in M. oryzae and potentially regulated by the Pmk1 mitogen-activated protein kinase pathway. We observed that Δznf1 mutants are affected in mitosis and impaired in mobilization and degradation of lipid droplets and glycogen reserves during appressorium differentiation. Site-directed mutagenesis confirmed that three of the four C2H2 zinc-finger domains are essential for the function of Znf1. Taken together, we conclude that a C2H2 zinc-finger TF encoded by ZNF1 is essential for appressorium development by the rice blast fungus.

Sign in / Sign up

Export Citation Format

Share Document