scholarly journals Identification of Carnation mottle virus from Lisianthus Plants in Taiwan

Plant Disease ◽  
2011 ◽  
Vol 95 (8) ◽  
pp. 1036-1036 ◽  
Author(s):  
Y.-K. Chen ◽  
Y.-S. Chang ◽  
C.-C. Chen
Keyword(s):  
Coat Protein ◽  
Mosaic Virus ◽  
Full Length ◽  
Mottle Virus ◽  
Yellow Mosaic Virus ◽  
Tomato Mosaic Virus ◽  
Genomic Rna ◽  
Local Lesions ◽  
Wilt Virus ◽  
Carnation Mottle Virus

Lisianthus (Eustoma exaltatum (L.) Salisb. ex G. Don subsp. russellianum (Hook.) Kartesz) is an economically important ornamental crop in Taiwan. Over the past decade, nine viruses have been identified or detected in lisianthus including: Bean yellow mosaic virus (BYMV), Lisianthus necrosis virus (LNV) (2), Cucumber mosaic virus (CMV) (1), Turnip mosaic virus (TuMV), Tomato spotted wilt virus (TSWV), Broad bean wilt virus (BBWV), Tomato mosaic virus (ToMV), Pepper veinal mottle virus (PVMV), and Ageratum yellow vein virus (AYVV) (4). In May 2007 (late period of growing season) in central Taiwan, systemic necrotic spots, which are similar to that caused by LNV (2), were found on approximately 20% of the lisianthus plants. Spherical virus particles, approximately 32 nm in diameter, were found in the crude sap of infected lisianthus collected from the fields. However, the diseased samples did not react with antisera against domestic lisianthus-infecting spherical viruses, LNV (2) and CMV (1). A virus culture was isolated via mechanical inoculation on Chenopodium quinoa and serologically identified as Carnation mottle virus (CarMV) by ELISA, western blotting, and immunoelectron microscopy using antiserum against the CarMV zantedeschia strain (3). The virus induced necrotic local lesions on the inoculated leaves of C. quinoa, C. amaranticolor, Gomphrena globosa, Cucurbita moschata, Phaseolus angularis, P. vulgaris, and Vigna unguiculata. Lisianthus was previously reported as a local lesion host for CarMV (3). In current studies with 8 of 10 lisianthus plants, the newly isolated virus induced necrotic local lesions on inoculated leaves 20 days post inoculation (dpi). However, systemic necrotic lesions on noninoculated upper leaves, as were observed in the fields, appeared 120 dpi on inoculated plants, indicating that CarMV induces systemic infection in lisianthus during late growth stages. Noninoculated plants did not develop symptoms. Complementary DNA fragments of viral genomic RNA were amplified with a specific primer of the coat protein gene (3) and sets of degenerate primer for CarMV. The amplified cDNA fragments were cloned and sequenced. The full-length sequence was submitted as GenBank Accession No. FJ843021. The genomic RNA consists of 4,003 nucleotides and has an identical genome organization to that reported for members of the genus Carmovirus. The nucleotide sequence of the full-length genome shares more than 95% identity to isolates of CarMV (GenBank Accession Nos. AF192772, AJ304989, AJ811998, NC_001265, and X02986), and the nucleotide and deduced amino acid sequence of coat protein shares more than 98% identity with that of CarMV-TW (AY383566) (3), CarMV-FO25 (EF622206), CarMV-Italy-Ca1 (EF622207), and CarMV-Netherland Ca2 (EF622210). To our knowledge, this is the first report of natural infection of CarMV in lisianthus in Taiwan. References: (1) C. C. Chen and C. C. Hu, Plant Prot. Bull. 41:179, 1999. (2) C. C. Chen et al. Plant Dis. 84:506, 2000. (3) C. C. Chen et al. Plant Dis. 87:1539, 2003. (4) Y. H. Cheng et al. J. Taiwan Agric. Res. 58:196, 2009.

scholarly journals First Report of Parietaria mottle virus on Tomato in Spain

Plant Disease ◽  
2001 ◽  
Vol 85 (11) ◽  
pp. 1210-1210 ◽  
Author(s):  
J. Aramburu
Keyword(s):  
Mosaic Virus ◽  
Polyclonal Antiserum ◽  
Mottle Virus ◽  
Tomato Mosaic Virus ◽  
Tobacco Streak Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Streak Virus ◽  
Range Data ◽  
Local Lesions ◽  
Plant Pathol

During spring 2001, plants of different tomato (Lycopersicon esculentum) cultivars grown in several commercial fields in the eastern Catalonia Region of Spain had fruit with brown patches and young leaves with rings and a bright necrotic mosaic that progressed to stem necrosis of the apex, which might die and later develop new symptomless shoots. The symptoms were similar to those of Cucumber mosaic virus (CMV) and Tomato spotted wilt virus (TSWV). Sap of tomato sample R1 (in buffered saline [0.02 M sodium phosphate, 0.15 M NaCl at pH 7.2, containing 0.2% 2-mercaptoethanol]) was infective to Cucumis sativus (local necrosis), tomato cv. Marmande (systemic infection consisting of chlorotic local lesions and necrotic mosaic), Nicotiana clevelandii and N. benthamiana (chlorosis and rosetting), and Chenopodium quinoa (chlorotic local lesions, systemic mottle, and leaf distortion). The sap was not infective to N. glutinosa, N. tabacum cv. Xanthi, Datura stramonium, or Gomphrena globosa. The host range data indicated that the infective agent in sample R1 could be Parietaria mottle virus (PMoV) (1). Symptomatic plants inoculated in a greenhouse with the R1 isolate and symptomatic from tomato plants from the field were analyzed by indirect enzyme-linked immunosorbent assay (ELISA) and had minimum ELISA values at least 10-fold higher than healthy controls, using a polyclonal antiserum (provided by P. Roggero) of a tomato strain of PMoV denoted tomato virus 1 (2). The R1 isolate of PMoV was negative in ELISA when analyzed with commercial antisera to TSWV, CMV, Tomato mosaic virus, Tomato bushy stunt virus, Potato Y virus, Tobacco etch virus, Pelargonium zonate spot virus, and Tobacco streak virus. References: (1) P. Caciagli et al. Plant Pathol. 38:577, 1989. (2) P. Roggero et al. J. Plant Pathol. 82:159, 2000.

scholarly journals First Report of Begonia Chlorotic Ringspot Caused by Zucchini yellow mosaic virus in Taiwan

Plant Disease ◽  
2008 ◽  
Vol 92 (8) ◽  
pp. 1247-1247 ◽  
Author(s):  
Y. K. Chen ◽  
Y. H. Hong
Keyword(s):  
Mosaic Virus ◽  
Early Stage ◽  
Natural Infection ◽  
Zucchini Yellow Mosaic Virus ◽  
Electron Microscopic Examination ◽  
Full Length ◽  
Yellow Mosaic Virus ◽  
Genomic Rna ◽  
Electron Microscopic ◽  
First Report

Begonia (Begonia semperflorens) showing symptoms of systemic chlorotic ringspots were observed in the central part of Taiwan in May 2006. Infected begonia plants showed faint ringspots in leaves at the early stage of infection. Symptoms turned chlorotic and coalesced as the disease progressed. Electron microscopic examination revealed filamentous virus particles approximately 750 × 13 nm in the crude sap of infected begonia. Typical pinwheel inclusion bodies of potyvirus infection were observed in the ultrathin sections of infected begonia. A virus culture was isolated via mechanical inoculations in Chenopodium quinoa and serologically identified as Zucchini yellow mosaic virus (ZYMV) (1) by ELISA, western blotting, and immunoelectron microscopy. Complementary DNA fragments of viral genomic RNA were cloned, sequenced, and the full-length sequence was submitted to the EMBL database (Accession No. AM422386). The genomic RNA consists of 9,591 nucleotides excluding the poly-A tail and has an identical genome organization to that reported for members of the genus, Potyvirus. The nucleotide sequence of the full-length genome and the deduced amino acid sequence of coat protein share 98% identity to those of ZYMV-CU (Accession No. AJ307036), ZYMV-SG (Accession No. AJ316228), and ZYMV-TW-TN3 (Accession No. AF127929). The virus caused local lesions on the inoculated leaves of C. quinoa, systemic mosaic in cucumber (Cucumis sativus), zucchini (Cucurbita pepo), and Cucumis metuliferus, and chlorotic ringspots in begonia. Symptoms caused by the begonia isolate in cucurbits were much milder than those caused by ZYMVs of cucurbit origin. A ringspot disease with symptoms similar to those caused by ZYMV is also produced on begonia by an Ilarvirus, Prunus necrotic ringspot virus (PNRSV), which was previously identified in begonia (2). To our knowledge, this is the first report of natural infection of ZYMV in begonia. References: (1) V. Lisa et al. Phytopathology 71:667, 1981. (2) N. Verma et al. Plant Pathol. 51:800, 2002.

scholarly journals Natural incidence of bean viruses in the northwest of Iran

2017 ◽  
Vol 109 (2) ◽  
pp. 331
Author(s):  
Mina Rastgou ◽  
Masoumeh Jalali
Keyword(s):  
Mosaic Virus ◽  
Bean Common Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Tomato Mosaic Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Growing Seasons ◽  
Tomato Spotted Wilt ◽  
Northwest Of Iran ◽  
Wilt Virus ◽  
Leaf Curl Virus

<p><span style="font-family: Times New Roman;">Bean is considered as one of the most important legumes around the world. Viral diseases are a major yield reducing factor in bean production. Bean samples with virus-like symptoms like severe or mild mosaic, vein banding, leaf curling, blistering and necrosis were collected from different bean fields in Urmia (Northwest of Iran) during the growing seasons of 2013 and 2014. <em>Bean common mosaic virus</em> (BCMV), <em>Bean common mosaic necrosis virus</em> (BCMNV), <em>Bean yellow mosaic virus</em> (BYMV), <em>Cucumber mosaic virus</em> (CMV), <em>Tomato spotted wilt virus</em> (TSWV), <em>Tomato mosaic virus</em> (ToMV) and <em>Tomato yellow leaf curl virus</em> (TYLCV) were detected by double antibody sandwich enzyme-linked-immunosorbent assay. Mixed infection of BCMV and BCMNV were found. BCMNV was the most frequent virus in this region whereas BYMV and TYLCV were each detected just in one sample. This is the first report of BCMNV, BCMV, BYMV, TSWV, TMV and TYLCV incidence on bean in Urmia, Iran.</span></p>

scholarly journals The Complete Nucleotide Sequence of Bean Yellow Mosaic Virus Genomic RNA.

1996 ◽  
Vol 62 (5) ◽  
pp. 472-477 ◽  
Author(s):  
Shigeo NAKAMURA ◽  
Ryoso HONKURA ◽  
Takayoshi IWAI ◽  
Masashi UGAKI ◽  
Yuko OHASHI
Keyword(s):  
Nucleotide Sequence ◽  
Mosaic Virus ◽  
Complete Nucleotide Sequence ◽  
Bean Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Genomic Rna

scholarly journals Synthesis of an infectious full-length cDNA clone of rice yellow mottle virus andmutagenesis of the coat protein

Virology ◽  
1995 ◽  
Vol 206 (1) ◽  
pp. 108-115 ◽  
Author(s):  
C. Brugidou ◽  
C. Holt ◽  
M. Ngon A Yassi ◽  
S. Zhang ◽  
R. Beachy ◽  
...  
Keyword(s):  
Coat Protein ◽  
Cdna Clone ◽  
Full Length ◽  
Mottle Virus ◽  
Rice Yellow Mottle Virus ◽  
Full Length Cdna ◽  
Yellow Mottle

scholarly journals Tropical soda apple mosaic virus Identified in Solanum capsicoides in Florida

Plant Disease ◽  
2007 ◽  
Vol 91 (9) ◽  
pp. 1204-1204 ◽  
Author(s):  
S. Adkins ◽  
G. McAvoy ◽  
E. N. Rosskopf
Keyword(s):  
Sequence Analysis ◽  
Mosaic Virus ◽  
Sandwich Elisa ◽  
Tomato Mosaic Virus ◽  
Rt Pcr ◽  
Degenerate Primers ◽  
Lee County ◽  
Cp Gene ◽  
Local Lesions ◽  
Tropical Soda Apple

Red soda apple (Solanum capsicoides All.), a member of the Solanaceae, is a weed originally from Brazil (3). It is a perennial in southern Florida and is characterized by abundant prickles on stems, petioles, and leaves. Prickles on stems are more dense than those on its larger, noxious weed relative, tropical soda apple (Solanum viarum Dunal), and the mature red soda apple fruits are bright red in contrast to the yellow fruits of tropical soda apple (2). Virus-like foliar symptoms of light and dark green mosaic were observed on the leaves of a red soda apple in a Lee County cow pasture during a tropical soda apple survey during the fall of 2004. The appearance of necrotic local lesions following inoculation of Nicotiana tabacum cv. Xanthi nc with sap from the symptomatic red soda apple leaves suggested the presence of a tobamovirus. Tropical soda apple mosaic virus (TSAMV), a recently described tobamovirus isolated from tropical soda apple in Florida, was specifically identified by a double-antibody sandwich-ELISA (1). An additional six similarly symptomatic red soda apple plants were later collected in the Devils Garden area of Hendry County. Inoculation of N. tabacum cv. Xanthi nc with sap from each of these symptomatic plants also resulted in necrotic local lesions. Sequence analysis of the TSAMV coat protein (CP) gene amplified from total RNA by reverse transcription (RT)-PCR with a mixture of upstream (SolA5′CPv = 5′-GAACTTWCAGAAGMAGTYGTTGATGAGTT-3′; SolB5′CPv = 5′-GAACTCACTGARRMRGTTGTTGAKGAGTT-3′) and downstream (SolA3′CPvc = 5′-CCCTTCGATTTAAGTGGAGGGAAAAAC-3′; SolB3′CPvc = 5′-CGTTTMKATTYAAGTGGASGRAHAAMCACT-3′) degenerate primers flanking the CP gene of Solanaceae-infecting tobamoviruses confirmed the presence of TSAMV in all plants from both locations. Nucleotide and deduced amino acid sequences of the 483-bp CP gene were both 98 to 99% identical to the original TSAMV CP gene sequences in GenBank (Accession No. AY956381). TSAMV was previously identified in tropical soda apple in these two locations in Lee and Hendry counties and three other areas in Florida (1). Sequence analysis of the RT-PCR products also revealed the presence of Tomato mosaic virus in the plant from Lee County. To our knowledge, this represents the first report of natural TSAMV infection of any host other than tropical soda apple and suggests that TSAMV may be more widely distributed in solanaceous weeds than initially reported. References: (1) S. Adkins et al. Plant Dis. 91:287, 2007. (2) N. Coile. Fla. Dep. Agric. Consum. Serv. Div. Plant Ind. Bot. Circ. 27, 1993. (3) U.S. Dep. Agric., NRCS. The PLANTS Database. National Plant Data Center. Baton Rouge, LA. Published online, 2006.

Transformation of Wheat With the Gene Encoding the Coat Protein of Barley Yellow Mosaic Virus

1996 ◽  
Vol 23 (4) ◽  
pp. 429 ◽  
Author(s):  
S Karunaratne ◽  
A Sohn ◽  
A Mouradov ◽  
J Scott ◽  
HH Steinbiss ◽  
...  
Keyword(s):  
Gene Expression ◽  
Coat Protein ◽  
Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Bar Gene ◽  
Gene Encoding ◽  
Barley Yellow Mosaic Virus ◽  
Virus Coat Protein ◽  
Wheat Embryos ◽  
Virus Coat

Wheat plants (Triticum aestivum cv. Hartog) were stably transformed with the bar gene and the gene encoding the barley yellow mosaic virus coat protein. Cultured immature wheat embryos were bombarded with tungsten particles coated with the pEmuPAT-cp construct. Fifteen regenerating 'PPT- resistant' plants were selected on medium containing phosphinothricin. Of these, 11 plants had both the bar and cp genes integrated into the wheat genome and two plants had only the bar gene. Transmission of the two genes to progeny of two independent plants was confirmed. The barley yellow mosaic virus coat protein was detected in both the parent and progeny plants; however, bar gene expression occurred only in the parent plants.

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