Characterization of Pythium Species Collected from a Multiple Time-Point Sampling of Cucurbits in South Carolina

Species of Pythium cause root and stem rot in cucurbits, but no formal surveys have been conducted in the United States to identify which species are responsible. The cucurbit hosts bottle gourd, cucumber, Hubbard squash, and watermelon were transplanted in May, July, September, and November into sentinel plots in four and five different fields in 2017 and 2018, respectively, in South Carolina. Eight of the nine fields were replanted in March 2019. Isolates (600) were collected and identified by sequencing DNA of the mitochondrial cytochrome oxidase I region. The four most common species were P. spinosum (45.6% of all isolates), P. myriotylum (20.0%), P. irregulare (15.3%), and P. aphanidermatum (12.8%). P. myriotylum and P. aphanidermatum were predominantly isolated in May, July, and September, whereas P. spinosum and P. irregulare were predominantly isolated in November and March. Isolates of P. ultimum, P. irregulare, and P. spinosum were more virulent than isolates of P. myriotylum and P. aphanidermatum at 25°C. Representative isolates were screened in vitro for sensitivity to three fungicides: mefenoxam, propamocarb, and oxathiapiprolin. All isolates were sensitive to mefenoxam and propamocarb, but these same isolates were insensitive to oxathiapiprolin, except those classified taxonomically in Pythium clade I.

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Characterization of transition diets spanning infancy and toddlerhood: a novel, multiple-time-point application of principal components analysis

American Journal of Clinical Nutrition ◽

10.3945/ajcn.111.026575 ◽

2012 ◽

Vol 95 (5) ◽

pp. 1200-1208 ◽

Cited By ~ 17

Author(s):

Laima Brazionis ◽

Rebecca K Golley ◽

Murthy N Mittinty ◽

Lisa G Smithers ◽

Pauline Emmett ◽

...

Keyword(s):

Principal Components Analysis ◽

Principal Components ◽

Multiple Time ◽

Multiple Time Point ◽

Components Analysis ◽

Time Point

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Multiple Time-Point 68Ga-PSMA I&T PET/CT for Characterization of Primary Prostate Cancer

Clinical Nuclear Medicine ◽

10.1097/rlu.0000000000001589 ◽

2017 ◽

Vol 42 (6) ◽

pp. e286-e293 ◽

Cited By ~ 16

Author(s):

Sebastian Schmuck ◽

Martin Mamach ◽

Florian Wilke ◽

Christoph A. von Klot ◽

Christoph Henkenberens ◽

...

Keyword(s):

Prostate Cancer ◽

Multiple Time ◽

Multiple Time Point ◽

Primary Prostate Cancer ◽

Pet Ct ◽

Time Point

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Transformation of hamster embryo cells by chymotrypsin-treated and untreated polyoma virus: characterization of transformants

Canadian Journal of Microbiology ◽

10.1139/m85-068 ◽

1985 ◽

Vol 31 (4) ◽

pp. 356-360

Author(s):

Vera Chlumecky ◽

Donald C. Stranks ◽

John S. Colter

Keyword(s):

Common Species ◽

Soft Agar ◽

T Antigen ◽

Polyoma Virus ◽

Large T Antigen ◽

T Antigens ◽

Hamster Embryo Cells ◽

Embryo Cells

The ability of chymotrypsin-treated (chymo+) and untreated (chymo−) polyoma virus to transform cultured hamster embryo fibroblasts was examined. The data show that exposure to this protease reduces the ability of the virus to transform non-permissive cells to essentially the same extent as it reduces its ability to replicate in permissive cells. Twenty-five lines of transformed cells were established from colonies growing in soft agar, and after 20 in vitro passages, cells of all lines were characterized with respect to their ability to form colonies in soft agar and their tumorigenicity in hamsters. While the studies showed that there are striking differences among the lines with respect to colony-forming ability, and real, though less striking differences in tumorigenicity, they failed to reveal any obvious differences between the groups of cell lines transformed by chymo− and chymo+ polyoma virus. Of 13 lines examined, all were found to express both middle and small polyoma T antigens, none express significant levels of large T antigen, and 11 express some form of what is probably a truncated large T antigen, the most common species having a molecular weight of 67 000.

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Multiple time-point assessment of lncRNA MEG3 shows potential to monitor treatment efficacy in rheumatoid arthritis patients

Biomarkers in Medicine ◽

10.2217/bmm-2021-0070 ◽

2021 ◽

Author(s):

Zehui Wang ◽

Weiyong Zhang ◽

Shouxia Li ◽

Dingli Chen ◽

Lei Wang ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Peripheral Blood ◽

Treatment Efficacy ◽

Multiple Time ◽

Clinical Role ◽

Multiple Time Point ◽

Potential Biomarker ◽

Monitoring Treatment ◽

Time Point

Background: This study explored the clinical role of lncRNA MEG3 in rheumatoid arthritis (RA) management. Materials & methods: Totally, 191 active RA patients were enrolled, and their lncRNA MEG3 expressions in peripheral blood monoclonal cells were detected. Results: LncRNA MEG3 expression was downregulated, and it negatively correlated with lesion joints, inflammation and disease activity in RA patients. Moreover, lncRNA MEG3 expression was increased during treatment; meanwhile its increment correlated with treatment response and remission. Conclusion: LncRNA MEG3 may serve as a potential biomarker for monitoring treatment efficacy in RA management.

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Virulence and Genetic Characterization of Six Baculovirus Strains Isolated From Different Populations of Spodoptera Frugiperda (Lepidoptera: Noctuidae)

10.21203/rs.3.rs-988050/v1 ◽

2021 ◽

Author(s):

Ingrid Zanella-Saenz ◽

Elisabeth A. Herniou ◽

Jorge E. Ibarra ◽

Ma.Cristina Del Rincón-Castro ◽

Ilse Alejandra Huerta-Arredondo

Keyword(s):

Biological Control ◽

Cell Cultures ◽

Spodoptera Frugiperda ◽

Fall Armyworm ◽

The United States ◽

Infection Mechanisms ◽

Different Populations

Abstract Fall armyworm (FAW), Spodoptera frugiperda (Smith, 1797), is a polyphagous, voracious, and economically important agricultural pest. Biological control of FAW is a strategy that must be further explored. This study evaluated six baculovirus strains isolated from infected FAW larvae from Mexico, Argentina, Honduras, and the United States. Five alphabaculoviruses (SfNPV-An2, SfNPV-Arg, SfNPV-Fx, SfNPV-Ho and SfNPV-Sin) and one betabaculovirus (SfGV-RV), were tested against FAW larvae, showing a wide diversity of virulence levels among strains when their estimated LC50s were compared, being SfNPVArg, SfNPV-Ho and SfNPV-Fx more virulent than SfNPV-An 2 , SfNPV-Sin and SfGV-RV. To determine any virulence difference in vitro studies of these isolates, Sf9 cell cultures were used. Interestingly, only ODVs from four of the test SfNPV strains showed infectivity on Sf9 cell cultures, and some differences in virulence were observed. Genomic restriction analyses and partial sequences of lef-8, lef-9 , and polh/granulin genes showed little variability among alphabaculoviruses, both, among them and with previously reported sequences. However, sequences from SfGV-RV were closer to previously reported sequences from the SfGVVG008 strain than the SfGV-Arg and SfGV-VG014 strains. The great difference in the in vivo virulence was not correlated with great similarity among the isolates. The characterization of these six baculoviruses isolates offers the basis for exploring their potential as biological control agents against S. frugiperda, as well the initial studies on their specific infection mechanisms, evolution, and ecology.

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P1-287: Proton spectroscopy in Alzheimer's disease: A longitudinal multiple time point study

Alzheimer s & Dementia ◽

10.1016/j.jalz.2008.05.877 ◽

2008 ◽

Vol 4 ◽

pp. T301-T302

Author(s):

Jonathan M. Schott ◽

David G. MacManus ◽

Chris Frost ◽

Martin N. Rossor ◽

Adam D. Waldman ◽

...

Keyword(s):

Alzheimer’S Disease ◽

Alzheimer's Disease ◽

Multiple Time ◽

Proton Spectroscopy ◽

Multiple Time Point ◽

Time Point

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Characterization of Leaf Spot Pathogens from Several Spinach Production Areas in the United States

Plant Disease ◽

10.1094/pdis-11-19-2450-re ◽

2020 ◽

Vol 104 (7) ◽

pp. 1994-2004

Author(s):

Bo Liu ◽

Larry Stein ◽

Kimberly Cochran ◽

Lindsey J. du Toit ◽

Chunda Feng ◽

...

Keyword(s):

United States ◽

South Carolina ◽

Leaf Spot ◽

The United States ◽

Leaf Damage ◽

Leaf Spots ◽

Pathogenicity Tests ◽

Agar Media ◽

Production Areas

Leaf spot diseases have become a major concern in spinach production in the United States. Determining the causal agents of leaf spots on spinach, their prevalence and pathogenicity, and fungicide efficacy against these pathogens is vital for effective disease management. Spinach leaves with leaf spots were collected from Texas, California, Arizona, and South Carolina from 2016 to 2018, incubated in a moist chamber, and plated on potato dextrose and tryptic soy agar media. Fungal and bacterial colonies recovered were identified based on morphology and sequence analysis of the internal transcribed spacer rDNA and 16S rRNA, respectively. Two predominant genera were isolated: (i) Colletotrichum spp., which were identified to species based on sequences of both introns of the glutamate synthetase (GS-I) and glyceraldehyde-3-phosphate dehydrogenase (gapdh-I) genes; and (ii) Stemphylium spp., identified to species based on sequences of the gapdh and calmodulin (cmdA) genes. Anthracnose (Colletotrichum spinaciae) and Stemphylium leaf spot (Stemphylium vesicarium and S. beticola) were the predominant diseases. Additional fungi recovered at very limited frequencies that were also pathogenic to spinach included Colletotrichum coccodes, C. truncatum, Cercospora beticola, and Myrothecium verrucaria. All of the bacterial isolates were not pathogenic on spinach. Pathogenicity tests showed that C. spinaciae, S. vesicarium, and S. beticola caused significant leaf damage. The fungicides Bravo WeatherStik (chlorothalonil), Dithane F-45 (mancozeb), Cabrio (pyraclostrobin), and Merivon (fluxapyroxad and pyraclostrobin) were highly effective at reducing leaf spot severity caused by an isolate of each of C. spinaciae and S. vesicarium, when inoculated individually and in combination.

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Characterization of Vancomycin-Resistant Enterococcus faecium Isolates from the United States and Their Susceptibility In Vitro to Dalfopristin-Quinupristin

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.42.5.1088 ◽

1998 ◽

Vol 42 (5) ◽

pp. 1088-1092 ◽

Cited By ~ 55

Author(s):

G. M. Eliopoulos ◽

C. B. Wennersten ◽

H. S. Gold ◽

T. Schülin ◽

M. Souli ◽

...

Keyword(s):

United States ◽

Enterococcus Faecium ◽

Multiple Drug Resistance ◽

The United States ◽

Multiple Drug ◽

Data Set ◽

Vancomycin Resistant ◽

High Level

ABSTRACT In the course of clinical studies with the investigational streptogramin antimicrobial dalfopristin-quinupristin, isolates of vancomycin-resistant Enterococcus faecium were referred to our laboratory from across the United States. Seventy-two percent of the strains were of the VanA type, phenotypically and genotypically, while 28% were of the VanB type. High-level resistance to streptomycin or gentamicin was observed in 86 and 81%, respectively, of the VanA strains but in only 69 and 66%, respectively, of the VanB strains. These enterococci were resistant to ampicillin (MIC for 50% of the isolates tested [MIC50] and MIC90, 128 and 256 μg/ml, respectively) and to the other approved agents tested, with the exception of chloramphenicol (MIC90, 8 μg/ml) and novobiocin (MIC90, 1 μg/ml). Considering all of the isolates submitted, dalfopristin-quinupristin inhibited 86.4% of them at concentrations of ≤1 μg/ml and 95.1% of them at ≤2 μg/ml. However, for the data set comprised of only the first isolate submitted for each patient, 94.3% of the strains were inhibited at concentrations of ≤1 μg/ml and 98.9% were inhibited at concentrations of ≤2 μg/ml. Multiple drug resistance was very common among these isolates of vancomycin-resistant E. faecium, while dalfopristin-quinupristin inhibited the majority at concentrations that are likely to be clinically relevant.

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Well-being, Smartphone Sensors, and Data from Open-access Databases: A Mobile Experience Sampling Study

Field Methods ◽

10.1177/1525822x18824281 ◽

2019 ◽

Vol 31 (3) ◽

pp. 277-291 ◽

Cited By ~ 4

Author(s):

Stefan Stieger ◽

Ulf-Dietrich Reips

Keyword(s):

Open Access ◽

Sampling Method ◽

Experience Sampling ◽

Past Research ◽

Well Being ◽

Multiple Time ◽

Multiple Time Point ◽

Smartphone Sensors ◽

Internet Databases ◽

Time Point

We investigated fluctuations of well-being by using a smartphone-based mobile experience sampling method (real-time and multiple time point measurements in the field using smartphones). Moreover, temperature, longitude, latitude, altitude, wind speed, rainfall, and further environment-based indicators were included as predictors either from smartphone sensors or from open-access Internet databases. Overall, a total of 213 participants reported on their well-being (over 14 days; three measurements per day; 8,000+ well-being judgments). We were able to replicate and refine past research about the dynamics of well-being fluctuations during the day (low in the morning, high in the evening) and over the course of a week (low just before the beginning of the week, highest near the end of the week). We also show what kind of benefits empirical researchers can gain for their research using smartphones and their built-in sensors by combining these measures with data from open-access databases.

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