scholarly journals First Report of the Cereal Cyst Nematode Heterodera filipjevi on Wheat in Serbia

Plant Disease ◽  
2012 ◽  
Vol 96 (10) ◽  
pp. 1583-1583 ◽  
Author(s):  
V. Oro ◽  
S. Živković ◽  
Ž. Ivanović ◽  
L. Waeyenberge
Keyword(s):  
New York ◽  
Tail Length ◽  
Its Region ◽  
Molecular Data ◽  
Cyst Nematode ◽  
Cereal Cyst Nematode ◽  
Important Species ◽  
First Report ◽  
Wheat Field ◽  
Heterodera Filipjevi

The most globally recognized and economically important nematode on wheat is the cereal cyst nematode (CCN) complex (1). One of the most important species of this group is Heterodera filipjevi (Madžidov, 1981) Mulvey and Golden, 1983. During regular soil quarantine control in September 2010, Heterodera sp. cysts were found in soil samples originating from a wheat field in Gunaroš, Vojvodina Province, in northern Serbia. The wheat was a winter crop grown in a dryland production system and had an average cyst density of 2.50/100 cm3 of soil. Morphologically, the cysts were golden brown and lemon shaped with a posterior protuberance. The vulval cone was bifenestrate with horseshoe-shaped semifenestra, bullae, and underbridge. Cyst measurements (n = 30) ranged as follows: cyst length (without neck): 511.50 to 899.00 μm, cyst width: 201.50 to 682.00 μm, fenestral length: 44.80 to 65.60 μm, fenestral width: 24.00 to 40.00 μm, vulval bridge length: 12.80 to 20.80 μm, vulval bridge width: 6.40 to 14.40 μm, vulval slit: 6.00 to 12.80 μm, and underbridge length: 60.00 to 112.00 μm. The second-stage juveniles had an offset head, stylet with characteristic anchor-shaped basal knobs, four incisures, and a conical tail with a rounded tip. The J2 morphometrics (n = 30) were: length: 447.30 to 611.10 μm, width: 22.40 to 25.60 μm, stylet: 20.80 to 24.00 μm, tail length: 56.00 to 68.80 μm, tail width: 14.40 to 19.20 μm, and hyaline length: 35.20 to 44.80 μm. The ITS region was used for molecular analysis. Each DNA sample was extracted from a single cyst. Sequencing was done with primers TW81 and AB28 (2). In comparison with other H. filipjevi populations, the obtained sequence (GenBank Accession No. JX235959) revealed 99 to 100% similarity. Morphological and molecular data confirmed the existence of H. filipjevi. This is, to our knowledge, the first report of H. filipjevi from Serbia. Since wheat has important socioeconomic value for Serbia, after extensive surveys, additional phytosanitary measures may be necessary to prevent the spread of this parasite. References: (1) J. M. Nicol et al. Current Nematode Threats to World Agriculture. Genomics and Molecular Genetics of Plant-Nematode Interactions, Springer, New York, 2011. (2) A. M. Skantar et al. J. Nematol. 39:133, 2007.

scholarly journals First Report of the Cereal Cyst Nematode Heterodera filipjevi on Winter Wheat in Montana

Plant Disease ◽  
2015 ◽  
Vol 99 (8) ◽  
pp. 1188-1188 ◽  
Author(s):  
A. T. Dyer ◽  
R. Al-Khafaji ◽  
T. Lane ◽  
T. Paulitz ◽  
Z. A. Handoo ◽  
...  
Keyword(s):  
Winter Wheat ◽  
Cyst Nematode ◽  
Cereal Cyst Nematode ◽  
First Report ◽  
Heterodera Filipjevi

scholarly journals First report of cereal cyst nematode (Heterodera filipjevi) on winter wheat in Shandong Province, China

2018 ◽  
Vol 17 (8) ◽  
pp. 1912-1913 ◽  
Author(s):  
Hao-yang ZHEN ◽  
Huan PENG ◽  
Hong-hai ZHAO ◽  
Yong-hong QI ◽  
Wen-kun HUANG ◽  
...  
Keyword(s):  
Winter Wheat ◽  
Cyst Nematode ◽  
Shandong Province ◽  
Cereal Cyst Nematode ◽  
First Report ◽  
Heterodera Filipjevi

scholarly journals First Report of Cereal Cyst Nematode, Heterodera filipjevi, on Winter Wheat from Anhui Province, China

Plant Disease ◽  
2016 ◽  
Vol 100 (2) ◽  
pp. 536 ◽  
Author(s):  
H. Peng ◽  
X. Li ◽  
J. K. Cui ◽  
D. L. Peng ◽  
W. K. Huang ◽  
...  
Keyword(s):  
Winter Wheat ◽  
Cyst Nematode ◽  
Anhui Province ◽  
Cereal Cyst Nematode ◽  
First Report ◽  
Heterodera Filipjevi

scholarly journals Diversity of Mycobiota Associated with the Cereal Cyst Nematode Heterodera filipjevi Originating from Some Localities of the Pannonian Plain in Serbia

Biology ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 283
Author(s):  
Violeta Oro ◽  
Rade Stanisavljevic ◽  
Bogdan Nikolic ◽  
Marijenka Tabakovic ◽  
Mile Secanski ◽  
...  
Keyword(s):  
Its Region ◽  
Fungal Species ◽  
Cyst Nematode ◽  
Cereal Cyst Nematode ◽  
Fungal Isolation ◽  
The Balkans ◽  
Pannonian Plain ◽  
Number Of Microorganisms ◽  
Pcr Products ◽  
Heterodera Filipjevi

Cereals, particularly wheat, are staple food of the people from the Balkans, dating back to the Neolithic age. In Serbia, cereals are predominantly grown in its northern part between 44° and 45.5° N of the Pannonian Plain. One of the most economically important nematodes on wheat is the cereal cyst nematode, Heterodera filipjevi. Cysts of H. filipjevi survive in soil for years and shelter a large number of microorganisms. The aims of this study were to investigate the diversity of mycobiota associated with the cereal cyst nematode H. filipjevi, to infer phylogenetic relationships of the found mycobiota, and to explore the ecological connection between fungi and the field history, including the potential of fungi in bioremediation and the production of novel bioactive compounds. Cysts were isolated from soil samples with a Spears apparatus and collected on a 150-µm sieve. The cysts were placed on potato dextrose agar, and maintained for two weeks at 27°C. Following fungal isolation and colony growing, the fungal DNA was extracted, the ITS region was amplified, and PCR products were sequenced. The study showed that the isolated fungal species belong to diverse phyla, including Ascomycota, Basidiomycota, and Mucoromycota. Ascomycota is represented by the families Clavicipitaceae, Sarocladiaceae, Nectriaceae, and Phaeosphaeriaceae. Basidiomycota is represented by the families Cerrenaceae, Polyporaceae, Phanerochaetaceae, and Meruliaceae, and the order Cantharellales. The family Mortierellaceae represents Mucoromycota. The members of Ascomycota and Basidiomycota both depict the field history. Ascomycota indicate the fungal infection is of recent origin, while Basidiomycota point toward the preceding host plants, enabling the plant field colonization history to be traced chronologically.

scholarly journals First Report of the Cereal Cyst Nematode (Heterodera filipjevi) on Wheat in Algeria

Plant Disease ◽  
2018 ◽  
Vol 102 (9) ◽  
pp. 1860-1860 ◽  
Author(s):  
D. Smaha ◽  
F. Mokrini ◽  
M. Imren ◽  
A. Mokabli ◽  
A. A. Dababat
Keyword(s):  
Cyst Nematode ◽  
Cereal Cyst Nematode ◽  
First Report ◽  
Heterodera Filipjevi

A GWAS to identify the cereal cyst nematode (Heterodera filipjevi) resistance loci in diverse wheat prebreeding lines

2021 ◽  
Author(s):  
Abdelfattah Dababat ◽  
Mian Abdur Rehman Arif ◽  
Halil Toktay ◽  
Osameh Atiya ◽  
Sajid Shokat ◽  
...  
Keyword(s):  
Cyst Nematode ◽  
Cereal Cyst Nematode ◽  
Heterodera Filipjevi

scholarly journals First Report of Stem Canker Disease of Pitaya (Hylocereus undatus and H. polyrhizus) Caused by Neoscytalidium dimidiatum in Taiwan

Plant Disease ◽  
2012 ◽  
Vol 96 (6) ◽  
pp. 906-906 ◽  
Author(s):  
M. F. Chuang ◽  
H. F. Ni ◽  
H. R. Yang ◽  
S. L. Shu ◽  
S. Y. Lai ◽  
...  
Keyword(s):  
New York ◽  
Aerial Mycelium ◽  
Its Region ◽  
Stem Canker ◽  
Canker Disease ◽  
First Report ◽  
Neoscytalidium Dimidiatum ◽  
Succulent Plant ◽  
Sterile Medium ◽  
Hylocereus Undatus

Pitaya (Hylocereus undatus and H. polyrhizus Britt. & Rose), a perennial succulent plant grown in the tropics, is becoming an emerging and important fruit plant in Taiwan. In September of 2009 and 2010, a number of pitaya plants were found to have a distinctive canker on stems. The disease expanded quickly to most commercial planting areas in Taiwan (e.g., Pintung, Chiayi, and Chunghua). Symptoms on the stem were small, circular, sunken, orange spots that developed into cankers. Pycnidia were erumpent from the surface of the cankers and the stems subsequently rotted. After surface disinfestation with 0.1% sodium hypochloride, tissues adjacent to cankers were placed on acidified potato dextrose agar (PDA) and incubated at room temperature for 1 week, after which colonies with dark gray-to-black aerial mycelium grew. Hyphae were branched, septate, and brown and disarticulated into 0- to 1-septate arthrospores. Sporulation was induced by culturing on sterile horsetail tree (Casuarina equisetifolia) leaves. Conidia (12.79 ± 0.72 × 5.14 ± 0.30 μm) from pycnidia were one-celled, hyaline, and ovate. The internal transcribed spacer (ITS) region of ribosomal DNA was PCR amplified with primers ITS1 and ITS4 (2) and sequenced. The sequence (GenBank Accession No. HQ439174) showed 99% identity to Neoscytalidium dimidiatum (Penz.) Crous & Slippers (GenBank Accession No. GQ330903). On the basis of morphology and nucleotide-sequence identity, the isolates were identified as N. dimidiatum (1). Pathogenicity tests were conducted in two replicates by inoculating six surface-sterilized detached stems of pitaya with either mycelium or conidia. Mycelial plugs from 2-day-old cultures (incubated at 25°C under near UV) were inoculated to the detached stems after wounding with a sterile needle. Conidial suspensions (103 conidia/ml in 200 μl) were inoculated to nonwounded stems. Noninoculated controls were treated with sterile medium or water. Stems were then incubated in a plastic box at 100% relative humidity and darkness at 30°C for 2 days. The symptoms described above were observed on inoculated stems at 6 to 14 days postinoculation, whereas control stems did not develop any symptoms. N. dimidiatum was reisolated from symptomatic tissues. To our knowledge, this is the first report of N. dimidiatum causing stem canker of pitaya. References: (1) P. W. Crous et al. Stud. Mycol. 55:235, 2006. (2) T. J. White et al. Page 315 in: PCR Protocols: A Guide to Methods and Applications. M. A. Innis et al., eds. Academic Press, New York, 1990.

Genetic variation and population dynamics of the cereal cyst nematode, Heterodera filipjevi in wheat areas of Bolu, Turkey

2017 ◽  
Vol 42 (5) ◽  
pp. 362-369 ◽  
Author(s):  
Mustafa Imren ◽  
Lieven Waeyenberge ◽  
A. Sami Koca ◽  
Nagihan Duman ◽  
Şenol Yildiz ◽  
...  
Keyword(s):  
Population Dynamics ◽  
Genetic Variation ◽  
Cyst Nematode ◽  
Cereal Cyst Nematode ◽  
Heterodera Filipjevi

Identification and characterisation of resistance to the cereal cyst nematode Heterodera filipjevi in winter wheat

Nematology ◽  
2016 ◽  
Vol 18 (4) ◽  
pp. 377-402 ◽  
Author(s):  
Shree R. Pariyar ◽  
Abdelfattah A. Dababat ◽  
Shahid Siddique ◽  
Gul Erginbas-Orakci ◽  
Abdelnaser Elashry ◽  
...  
Keyword(s):  
Cyst Nematode ◽  
Cereal Cyst Nematode ◽  
Root Weight ◽  
Sources Of Resistance ◽  
Plant Weight ◽  
Resistant Accession ◽  
Significant Difference ◽  
New Material ◽  
Heterodera Filipjevi ◽  
Moderately Resistant

The aim of this study was to search for new sources of resistance against the cereal cyst nematode,Heterodera filipjevi, in a collection of 290 wheat accessions. The plants were inoculated with juveniles and assessed for the number of females and cysts. One percent of the wheat accessions were ranked as resistant, 16% as moderately resistant, 41% as moderately susceptible, 26% as susceptible and 15% as highly susceptible. The infection rate and the number of females and cysts per plant were significantly lower in the resistant accession Nudakota and three moderately resistant accessions Ekonomka, Katea and Lantian 12 compared with susceptible cv. Bezostaya 1. Nematode development was reduced in resistant and moderately resistant accessions. The size of females and the total number of eggs and second-stage juveniles were reduced only in Ekonomka. No significant difference in plant height, plant weight, root length, root weight and root volume were recorded for inoculated plants compared to non-inoculated plants. This study has identified four resistant wheat accessions offering new material for breeding the resistance toH. filipjevi.

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