scholarly journals Smilax auriculata: A new host for Orchid fleck dichorhavirusvirus identified in Florida, USA

Plant Disease ◽  
2021 ◽  
Author(s):  
Kishore Dey ◽  
Maria Velez-Climent ◽  
Chellappan Padmanabhan ◽  
Schyler Nunziata ◽  
Yazmin Rivera ◽  
...  
Keyword(s):  
Nucleotide Identity ◽  
Rt Pcr ◽  
Illumina Platform ◽  
Department Of Agriculture ◽  
New Host ◽  
Alachua County ◽  
Subgroup Ii ◽  
Florida Department ◽  
Consumer Services ◽  
First Time

In June 2020, Orchid fleck virus (OFV) was detected in a species of Liriope in Leon and Alachua County, Florida (Fife et al; 2021). In October of the same year, four adjacent dune/ear-leaf greenbrier vines, Smilax auriculata (Smilaceae: Liliales), showed yellowing and mottling symptoms (Figure 1). Infected and healthy S. auriculata leaves samples were collected in Alachua County by the Florida Department of Agriculture and Consumer Services, Gainesville, Florida. OFV primers successfully detected in four Smilax samples by conventional RT-PCR assay. Amplicon sequences (Acc. No. MZ645935 and MZ645938) shared 99% nucleotide identity with OFV infecting orchids (LC222629) and citrus (MK522804). The OFV subgroup I (OFV-Orc1) and subgroup II (OFV-Orc2) specific primers (Kondo et al 2017) were utilized to confirm the presence of OFV type strains infecting Smilax. Sanger sequencing of subgroup I specific amplicons (MZ645934) shared 99% nucleotide identity with OFV-Orc1 (LC222629) whereas subgroup II specific amplicon sequence (MZ645930) shared 98-99 % nucleotide identity with OFV-Orc2 (AB244417). Further confirmation was done by USDA-APHIS-PPQ-Plant Pathogen Confirmatory Diagnostics Laboratory utilizing optimized conventional RT-PCR protocols (Roy et al. 2020) and deep sequencing on a on a NextSeq550 Illumina platform. Assembled reads identified seven non-overlapping viral contigs. Five RNA1 and two RNA2 contigs covered more than 97% of the bipartite OFV genome with average coverage depth of 5297.61 and 5186.04, respectively. Contigs of RNA1 and RNA2 shared 98-99% nt identity to OFV-Orc2-RNA1 (AB244417) and OFV-Orc-RNA2 (AB244418 and LC222630). No other pathogen sequences were identified. This is the first time the genus Smilax has been identified as a natural host of OFV. Very recent findings of OFV-Orc in Florida in Liriope, Aspidistra, and Ophiopogon among the Asparagaceae family members (Fife et al; 2021) and now in the Smilacaceae suggest a broader host range of the virus than previously known; further research should be conducted to better characterize the potential risk of introduction into citrus in Florida.

scholarly journals Lobate Lac Scale, Paratachardina lobata lobata (Chamberlin) (Hemiptera: Sternorrhyncha: Coccoidea: Kerriidae)

EDIS ◽  
1969 ◽  
Vol 2003 (6) ◽  
Author(s):  
Forrest W. Howard ◽  
Avas Hamon ◽  
Greg S. Hodges ◽  
Catharine M. Mannion ◽  
Jeanette Wofford
Keyword(s):  
Scale Insect ◽  
Cooperative Extension Service ◽  
Extension Service ◽  
Plant Industry ◽  
Department Of Agriculture ◽  
University Of Florida ◽  
Florida Department ◽  
Consumer Services ◽  
First Time ◽  
Trees And Shrubs

A species of scale insect new to Florida is potentially one of the most devastating pests of trees and shrubs in the state's history. The lobate lac scale, Paratachardina lobata lobata (Chamberlin) (Hemiptera: Sternorrhyncha: Coccoidea: Kerriidae), a scale insect native to India and Sri Lanka, was found for the first time in Florida in August 1999 by personnel of the Florida Department of Agriculture and Consumer Services, Division of Plant Industry (DPI) (Hamon 2001). This document is EENY-276, one of a series of the Department of Entomology, Florida Cooperative Extension Service, Institute of Food and Agricultural Sciences, University of Florida. Date printed: November 2002. EENY-276/IN471: Lobate Lac Scale, Paratachardina lobata lobata (Chamberlin) (Hemiptera: Sternorrhyncha: Coccoidea: Kerriidae) (ufl.edu)

scholarly journals Lobate Lac Scale, Paratachardina lobata lobata (Chamberlin) (Hemiptera: Sternorrhyncha: Coccoidea: Kerriidae)

EDIS ◽  
2019 ◽  
Vol 2005 (5) ◽  
Author(s):  
F. W. Howard ◽  
Robert Pemberton ◽  
Avas Hamon ◽  
Greg S. Hodges ◽  
Bryan Steinberg ◽  
...  
Keyword(s):  
Scale Insect ◽  
Cooperative Extension Service ◽  
Extension Service ◽  
Plant Industry ◽  
Department Of Agriculture ◽  
University Of Florida ◽  
Florida Department ◽  
Consumer Services ◽  
First Time ◽  
Trees And Shrubs

A species of scale insect new to Florida is potentially one of the most devastating pests of trees and shrubs in the state's history. The lobate lac scale, Paratachardina lobata lobata (Chamberlin) (Hemiptera: Sternorrhyncha: Coccoidea: Kerriidae), a scale insect native to India and Sri Lanka, was found for the first time in Florida in August 1999 by personnel of the Florida Department of Agriculture and Consumer Services, Division of Plant Industry (DPI) (Hamon 2001). This document is EENY-276, one of a series of the Department of Entomology, Florida Cooperative Extension Service, Institute of Food and Agricultural Sciences, University of Florida. Date printed: November 2002. Revised: May 2004. EENY-276/IN471: Lobate Lac Scale, Paratachardina lobata lobata (Chamberlin) (Hemiptera: Sternorrhyncha: Coccoidea: Kerriidae) (ufl.edu)

scholarly journals Licensing of Commensal Rodent Trappers

EDIS ◽  
2018 ◽  
Vol 2018 (5) ◽  
Author(s):  
Frederick M. Fishel
Keyword(s):  
Wildlife Management ◽  
Commercial Fertilizer ◽  
Department Of Agriculture ◽  
Certification Programs ◽  
Florida Department ◽  
Consumer Services ◽  
Commercial Landscape

The Florida Department of Agriculture and Consumer Services’ Bureau of Licensing and Enforcement (FDACS BLE) administers Limited Certification Categories to certify Governmental or Private applicators. Limited certification programs available are for Commercial Landscape Maintenance, Lawn and Ornamental, Structural, and Urban Commercial Fertilizer applicators, and Limited Commercial Wildlife Management trappers. This document will discuss the Limited Commercial Wildlife Management Trapper Certificate.

scholarly journals Hemp Fertilization: Current Knowledge, Gaps and Efforts in Florida: A 2020 Report

EDIS ◽  
2020 ◽  
Vol 2020 (4) ◽  
Author(s):  
Rao S. Mylavarapu ◽  
Zachary Brym ◽  
Luis Monserrate ◽  
Michael J. Mulvaney
Keyword(s):  
Current Knowledge ◽  
Pilot Project ◽  
Herbaceous Plant ◽  
Nutrient Requirements ◽  
Department Of Agriculture ◽  
Personal Communications ◽  
Specific Data ◽  
Florida Department ◽  
Consumer Services ◽  
Industrial Hemp

Hemp is an annual herbaceous plant that may be grown for fiber, seed, or flowers. Starting in April 2020, the Florida Department of Agriculture and Consumer Services began accepting applications for cultivation of hemp in Florida, with the potential for building a $20–$30 billion industry in the state. The statewide UF/IFAS Industrial Hemp Pilot Project is researching aspects of agronomic production for hemp cultivation. While a few other state soil testing laboratories provide soil tests and nutrient recommendations based on research and experience, at this time no Florida-specific data on nutrient requirements and fertilization are available. This new 5-page article, written by Rao Mylavarapu, Zachary Brym, Luis Monserrate, and Michael J. Mulvaney and published by the UF/IFAS Department of Soil and Water Sciences, provides a summary of published and personal communications from different states on hemp fertilization.https://edis.ifas.ufl.edu/ss689

scholarly journals 2021 Handbook of Florida Water Regulation: Florida Department of Agriculture and Consumer Services

EDIS ◽  
2021 ◽  
Vol 2021 (3) ◽  
pp. 2
Author(s):  
Michael T. Olexa ◽  
Tatiana Borisova ◽  
Jana Caracciolo
Keyword(s):  
Water Regulation ◽  
Department Of Agriculture ◽  
Florida Department ◽  
Consumer Services

This handbook is designed to provide a summary of the principal federal and state (Florida) laws that directly or indirectly relate to agriculture. Because these laws are subject to constant revision, portions of the handbook could become outdated at any time. The reader should use it as a means to determine areas in which to seek more information and as a brief directory of agencies that can help answer more specific questions.

scholarly journals Handbook of Florida Water Regulation: Florida Department of Agriculture and Consumer Services

EDIS ◽  
2019 ◽  
Vol 2006 (1) ◽  
Author(s):  
Michael T. Olexa ◽  
Luke D'Isernia ◽  
Laura Minton ◽  
Dulcy Miller ◽  
Sarah Corbett
Keyword(s):  
Resource Economics ◽  
Court Decisions ◽  
Water Regulation ◽  
Department Of Agriculture ◽  
Contact Information ◽  
Florida Department ◽  
Consumer Services ◽  
Economics Department ◽  
The Many ◽  
Farmland Owners

This handbook is designed to provide an accurate, current, and authoritative summary of the principle Federal and Florida laws that directly or indirectly relate to agriculture. This handbook should provide a basic overview of the many rights and responsibilities that farmers and farmland owners have under both Federal and Florida laws as well as the appropriate contact information to obtain more detailed information. However, the reader should be aware that because the laws, administrative rulings, and court decisions on which this handbook is based are subject to constant revision, portions of this publication could become outdated at anytime. Several details of cited laws are also left out due to space limitations. This document is FE596, one of a series of the Food and Resource Economics Department, UF/IFAS Extension. Published December 2005. FE596/FE596: 2021 Handbook of Florida Water Regulation: Florida Department of Agriculture and Consumer Services (ufl.edu)

scholarly journals Safe Handling of Food and Water in a Hurricane or Related Disaster

EDIS ◽  
2019 ◽  
Vol 2005 (13) ◽  
Author(s):  
Ronald H. Schmidt ◽  
Keith L. Schneider ◽  
Renée M. Goodrich ◽  
Amarat Simonne ◽  
Douglas L. Archer
Keyword(s):  
Environmental Protection Agency ◽  
Red Cross ◽  
Publication Date ◽  
American Red Cross ◽  
Department Of Agriculture ◽  
Safe Handling ◽  
Department Of Health ◽  
Florida Department ◽  
Consumer Services ◽  
Control And Prevention

This document contains a summary of general recommendations to protect the safety of food and water in the event of a hurricane, flooding, or related natural disaster. These recommendations have been adapted from those developed under National Recommendations for Disaster Food Handling, a multi-state grant involving Cooperative Extension collaborators from Tennessee, Florida, and Missouri as well as the American Red Cross and Tennessee Department of Agriculture. Recommendations have also been included from additional up-to-date sources such as the: Centers for Disease Control and Prevention (CDC), Environmental Protection Agency (EPA), Food and Drug Administration (FDA), Florida Department of Agriculture and Consumer Services (DOACS), Florida Department of Health (DOH), Federal Emergency Management Agency (FEMA), and American Red Cross. This document is FSHN0520, one of a series of the Food Science and Human Nutrition Department, UF/IFAS Extension. Original publication date October 2005. FSHN0520/FS131: Safe Handling of Food and Water in a Hurricane or Related Disaster (ufl.edu)

scholarly journals Multiresidue Analysis of Pesticides in Fresh Fruits and Vegetables Using Procedures Developed by the Florida Department of Agriculture and Consumer Services

1999 ◽  
Vol 82 (6) ◽  
pp. 1419-1435 ◽  
Author(s):  
Joanne Cook ◽  
Mary Pat Beckett ◽  
Brian Reliford ◽  
Walter Hammock ◽  
Marc Engel
Keyword(s):  
Fruits And Vegetables ◽  
Methylene Chloride ◽  
Solid Phase ◽  
Residue Analysis ◽  
Department Of Agriculture ◽  
Multiresidue Analysis ◽  
California Department ◽  
Florida Department ◽  
Consumer Services ◽  
Selective Detectors

Abstract Improved quality and efficiency of pesticide residue analysis were achieved by examining all aspects of the laboratory process. In an effort to eliminate methylene chloride hazardous waste, an acetonitrile extraction method, originally developed by the California Department of Agriculture, was modified and adopted. Sample size and solvent consumption were reduced with the new method. Custom glassware racks and disposable supplies reduced overall analysis time. Gravity-fed, solid-phase extraction simplified sample preparation and provided cleaner extracts for gas chromatographic analyses. Modifications to the method were made to achieve the ruggedness needed to maintain quality objectives during routine analysis. Instrumental improvements, including new selective detectors, retention time locking, and mass spectrometry screening for all samples, provided the laboratory with efficient, reliable, and confirmed analytical results.

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