First report of Mesta yellow vein mosaic virus, Cotton leaf curl Multan betasatellite and Cotton leaf curl Multan alphasatellite infecting cotton in Pakistan

Plant Disease ◽  
2014 ◽  
pp. 140122140029006 ◽  
Author(s):  
Usman Hameed ◽  
Muhammad Zia-Ur-Rehman ◽  
Hans-Werner Herrmann ◽  
Muhammad Saleem Haider ◽  
Judith K Brown
Keyword(s):  
Mosaic Virus ◽  
Yellow Vein ◽  
Cotton Leaf ◽  
First Report ◽  
Yellow Vein Mosaic Virus ◽  
Leaf Curl

scholarly journals First Report of Bhendi yellow vein mosaic virus Associated with Yellow Vein Mosaic of Okra (Abelmoschus esculentus) in Thailand

Plant Disease ◽  
2013 ◽  
Vol 97 (2) ◽  
pp. 291-291 ◽  
Author(s):  
W. S. Tsai ◽  
S. L. Shih ◽  
L. M. Lee ◽  
J. T. Wang ◽  
U. Duangsong ◽  
...  
Keyword(s):  
Southeast Asia ◽  
Mosaic Virus ◽  
Mosaic Disease ◽  
Yellow Vein ◽  
Abelmoschus Esculentus ◽  
Marketable Yield ◽  
Universal Primer ◽  
First Report ◽  
Conserved Sequence ◽  
Yellow Vein Mosaic Virus

A disease of okra (Abelmoschus esculentus) causing yellowing veins and mosaic on leaves and fruit has emerged in Thailand. Incidences of 50 to 100% diseased plants were observed in fields in Kanchanaburi and Nakhon Pathom provinces in 2009 and 2010, respectively. Leaf samples were collected from three and four diseased plants in Kanchanaburi and Nakhon Pathom, respectively. All seven samples tested positive for begomovirus by PCR using universal primer pair PAL1v1978B/PAR1c715H (3). One sample from Kanchanaburi also tested positive by ELISA using Okra mosaic virus (Genus Tymovirus) antiserum (DSMZ, Braunschweig, Germany). When the nucleotide sequences of the 1.5 kb begomovirus PCR products were compared they were found to share 99.1 to 99.5% identity with each other, and 97.5 to 97.7% identity to Bhendi yellow vein mosaic virus Okra isolate from India (GenBank Accession No. GU112057; BYVMV-[IN: Kai:OY: 06]). The complete DNA-A sequence for a Kanchanaburi isolate (JX678967) was obtained using abutting primers WTHOK6FL-V/-C (WTHOK6FL-V: 5′-GCGAAGCTTAGATAACGCTCCTT-3′; WTHOK6FL-C: 5′-TCCAAGCTTTGAGTCTGCAACGT-3′), while that of a Nakhon Pathom isolate (JX678966) was obtained with primers WTHOK6FLV/WTHOK2FL-C (WTHOK2FL-C: 5′-TCCAAGCTTTGAGTCTGCATCGT-3′). The DNA-A sequences of both isolates are 2,740 nucleotides in length and share 99.6% identity. Each has the geminivirus conserved sequence (TAATATTAC), two open reading frames (ORFs) in the virus sense (V1 and V2) and four in the complementary sense (C1 to C4). Based on BLASTn searching GenBank and sequence analysis using MegAlign (DNASTAR), both DNA-A sequences have greatest nucleotide identity (96.2 to 96.4%) with BYVMV-[IN: Kai:OY: 06] from India. Also, BYVMV-associated betasatellite DNA (1.4 kb) was detected in all begomovirus-positive samples, except one sample from Nakhon Pathom (1). However, no virus DNA-B was detected in any of the samples using either general detection primer pair DNABLC1/DNABLV2 or DNABLC2/DNABLV2 (2). Okra infected with BYVMV has been reported in South Asia in Bangladesh, India, and Pakistan. To the best of our knowledge, this is the first report of BYVMV associated with Okra Yellow Vein Mosaic Disease in Southeast Asia. Since fruits with symptoms are regarded as low quality and have little market value, even low incidence of the disease is likely to cause significant reductions in marketable yield. Strategies for managing BYVMV in okra in South and Southeast Asia should be sought, including the breeding and selecting of resistant varieties. References: (1) R. W. Briddon et al. Mol. Biotechnol. 20:315, 2002. (2) S. K. Green et al. Plant Dis. 85:1286, 2001. (3) W. S. Tsai et al. Plant Pathol. 60:787, 2011.

scholarly journals Identification and Functional Analysis of Four RNA Silencing Suppressors in Begomovirus Croton Yellow Vein Mosaic Virus

2022 ◽  
Vol 12 ◽  
Author(s):  
Ying Zhai ◽  
Anirban Roy ◽  
Hao Peng ◽  
Daniel L. Mullendore ◽  
Gurpreet Kaur ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Rna Silencing ◽  
Cell Movement ◽  
Yellow Vein ◽  
Silencing Suppressor ◽  
Monopartite Begomovirus ◽  
Yellow Vein Mosaic Virus ◽  
First Time ◽  
Leaf Curl Disease ◽  
Leaf Curl

Croton yellow vein mosaic virus (CYVMV), a species in the genus Begomovirus, is a prolific monopartite begomovirus in the Indian sub-continent. CYVMV infects multiple crop plants to cause leaf curl disease. Plants have developed host RNA silencing mechanisms to defend the threat of viruses, including CYVMV. We characterized four RNA silencing suppressors, namely, V2, C2, and C4 encoded by CYVMV and betasatellite-encoded C1 protein (βC1) encoded by the cognate betasatellite, croton yellow vein betasatellite (CroYVMB). Their silencing suppressor functions were verified by the ability of restoring the β-glucuronidase (GUS) activity suppressed by RNA silencing. We showed here for the first time that V2 was capable of self-interacting, as well as interacting with the V1 protein, and could be translocalized to the plasmodesmata in the presence of CYVMV. The knockout of either V2 or V1 impaired the intercellular mobility of CYVMV, indicating their novel coordinated roles in the cell-to-cell movement of the virus. As pathogenicity determinants, each of V2, C2, and C4 could induce typical leaf curl symptoms in Nicotiana benthamiana plants even under transient expression. Interestingly, the transcripts and proteins of all four suppressors could be detected in the systemically infected leaves with no correlation to symptom induction. Overall, our work identifies four silencing suppressors encoded by CYVMV and its cognate betasatellite and reveals their subcellular localizations, interaction behavior, and roles in symptom induction and intercellular virus movement.

Bhendi yellow vein mosaic virus and bhendi yellow vein mosaic betasatellite cause enation leaf curl disease and alter host phytochemical contents in okra

Plant Disease ◽  
2021 ◽  
Author(s):  
Achuit Kumar Singh ◽  
Brijesh K Yadav ◽  
Ram Krishna ◽  
R Vinoth Kumar ◽  
Gyan P Mishra ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Southern Hybridization ◽  
Indian Subcontinent ◽  
Uttar Pradesh ◽  
Yellow Vein ◽  
Monopartite Begomovirus ◽  
Yellow Vein Mosaic Virus ◽  
Dicotyledonous Plants ◽  
Leaf Curl Disease ◽  
Leaf Curl

Whitefly-transmitted begomoviruses cause severe diseases in numerous economically important dicotyledonous plants. In recent years, okra enation leaf curl disease (OELCuD) emerged as a serious threat to okra (Abelmoschus esculentus L. Moench) cultivation in the Indian subcontinent. The present study reports the association of a monopartite begomovirus (bhendi yellow vein mosaic virus - BYVMV) and betasatellite (bhendi yellow vein mosaic betasatellite - BYVB) with OELCuD in the Mau region of Uttar Pradesh, India. The BYVMV alone inoculated N benthamiana and A esculentus cv. Pusa Sawani plants developed mild symptoms. Co-inoculation of BYVMV and BYVB resulted in a reduced incubation period, an increased symptom severity and an enhanced BYVMV accumulation (by Southern hybridization and qPCR). This is the first study which satisfies Koch’s postulates for OELCuD in its natural host. Activities of various antioxidative enzymes were significantly increased in the virus inoculated okra plants. Differential responses in various biochemical components (such as photosynthetic pigments, phenol, proline, sugar) in diseased okra plants were observed. This change in phytochemical responses is of significant importance in understanding its impact on virus pathogenesis and disease development.

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