scholarly journals First Report of Tobacco streak virus in Strawberry in the Eastern United States

Plant Disease ◽  
2000 ◽  
Vol 84 (4) ◽  
pp. 488-488 ◽  
Author(s):  
S. C. Hokanson ◽  
R. R. Martin ◽  
J. L. Maas
Keyword(s):  
United States ◽  
Sandwich Elisa ◽  
Tobacco Streak Virus ◽  
Streak Virus ◽  
Eastern United States ◽  
Department Of Agriculture ◽  
Flat Bottom ◽  
Microtiter Plates ◽  
Elisa Plate

In a 1998 virus survey (2) conducted on 23 commercial strawberry (Fragaria × ananassa Duchesne) production farms in the state of Maryland, leaf samples from 1,100 randomly sampled plants were sent to the U.S. Department of Agriculture laboratory in Corvallis, OR, for testing by enzyme-linked immunosorbant assays (ELISA). The viruses identified were Strawberry mild yellow edge, Strawberry crinkle, Strawberry veinbanding, Strawberry mottle, and Tomato ringspot viruses, all of which are known in the eastern United States. Tobacco streak virus (TSV) also was identified in 17 of the samples: 12 originated from a 1-year-old planting of ‘Sweet Charlie’ and 5 from another farm, of which 4 were from a 2-year-old ‘Sweet Charlie’ planting and 1 was from a 2-year-old ‘Delmarvel’ planting. Triple antibody sandwich ELISA was used to detect TSV following the procedures described by Finn and Martin (1), except that leaves from test plants were homogenized (1:20, wt/vol, in blocking buffer) and flat bottom microtiter plates (Nunc, Roskilde, Denmark) and goat anti-mouse (polyvalent) alkaline phosphatase conjugate were used in the assays. The absorbance of each well at 405 nm (A405) was read in an ELISA plate reader. Reactions were considered positive if the A405 values were greater than five times the values of healthy samples. The A405 values of healthy samples ranged from 0.0 to 0.04, with values greater than 0.20 considered positive for TSV. An independent determination of TSV was made in plants shipped from Florida to Maryland in 1999. In this instance, leaf samples from ‘Sweet Charlie’ plants were sent by the Maryland Department of Agriculture to Agdia Inc. (Elkhart, IN), where samples tested positive for TSV. References: (1) C. E. Finn and R. R. Martin. Plant Dis. 80:769, 1996. (2) S. C. Hokanson, et al. Adv. Strawberry Res. In press.

scholarly journals First Report of Blackberry chlorotic ringspot virus in Rubus sp. in the United States

Plant Disease ◽  
2007 ◽  
Vol 91 (4) ◽  
pp. 463-463 ◽  
Author(s):  
I. E. Tzanetakis ◽  
J. D. Postman ◽  
R. R. Martin
Keyword(s):  
United States ◽  
The United States ◽  
Ringspot Virus ◽  
Tobacco Streak Virus ◽  
Rapid Decline ◽  
Streak Virus ◽  
Black Raspberry ◽  
Rt Pcr ◽  
Indicator Plants ◽  
First Report

Blackberry chlorotic ringspot virus (BCRV), genus Ilarvirus, has been found in Rubus sp. in Scotland (2) and rose in the United States (4). The possibility that BCRV infects other hosts in the United States was explored. We tested 18 accessions of Fragaria sp. and 30 of Rubus sp. maintained at the National Clonal Germplasm Repository in Corvallis, OR. Ilarviruses had been detected in these plants by reverse transcription (RT)-PCR, ELISA, or had caused symptoms typical of ilarviruses on indicator plants. The accessions were tested by RT-PCR with primers F (5′-GTTTCCTGTGCTCCTCA-3′) and R (5′-GTCACACCGAGGTACT-3′) (4) that amplify a 519 to 522 nt (depending on the isolate) region of the RNA 3 of BCRV. The virus was detected in two accessions of black raspberry (Rubus occidentalis L.): RUB433, cv. Lowden and RUB 9012, cv. New Logan. The sequences of the fragments amplified from these accessions (GenBank Accession Nos. EF041817 and EF041818, respectively) had 97% nt sequence identity to each other and 95 and 88% nt identity to the rose and Scottish isolates (GenBank Accession Nos. DQ329378 and DQ091195, respectively). Chenopodium quinoa indicator plants inoculated with isolate RUB 433 developed mild chlorotic spots on the inoculated leaves 4 days after inoculation. RT-PCR and sequencing of the amplicons verified BCRV infection of C. quinoa. RUB 9012 was used for the characterization of Black raspberry latent virus (BRLV), later thought to be an isolate of Tobacco streak virus (TSV). This accession was recently found to be infected with Strawberry necrotic shock virus (SNSV) but not TSV (3). It is possible that BRLV may be a mixture of SNSV and BCRV. SNSV is one of the most abundant viruses of Rubus sp. in the Pacific Northwest (1), and the finding of another ilarvirus, BCRV, may account in part for the rapid decline of Rubus sp. observed in several fields in Oregon and Washington. To our knowledge, this is the first report of BCRV infecting Rubus sp. outside the United Kingdom. References: (1) A. B. Halgren. Ph.D. Diss. Oregon State University, Corvallis, OR, 2006. (2) A. T. Jones et al. Ann. Appl. Biol. 149:125, 2006. (3) I. E. Tzanetakis et al. Arch. Virol. 149:2001, 2004. (4) I. E. Tzanetakis et al. Plant Pathol. 55:568, 2006.

scholarly journals Identification of Tobacco streak virus Associated With a Virus-like Mottle Symptom on Hosta

2013 ◽  
Vol 14 (1) ◽  
pp. 60 ◽  
Author(s):  
John R. Fisher
Keyword(s):  
United States ◽  
Capsid Protein ◽  
Movement Protein ◽  
The United States ◽  
Tobacco Streak Virus ◽  
Streak Virus ◽  
First Report ◽  
Leaf Symptom

In this brief, the authors describe the identification of Tobacco streak virus associated with a virus-like leaf symptom on Hosta spp. The identity of the virus was confirmed by ELISA and PCR using cDNAs synthesized from dsRNA template and immuncaptured virions and primers to amplify the movement protein and capsid protein genes. This is the first report of TSV infecting Hosta in Ohio, and to our knowledge in the United States. Accepted for publication 17 December 2012. Published 22 January 2013.

Occurrence of the Grape Cane Girdler, Ampeloglypter ater LeConte (Coleoptera: Curculionidae) in Ontario

1966 ◽  
Vol 98 (8) ◽  
pp. 880-880
Author(s):  
A. B. Stevenson
Keyword(s):  
United States ◽  
Lake Ontario ◽  
Eastern United States ◽  
Department Of Agriculture ◽  
Canada Department ◽  
Point Pelee ◽  
Research Institute

An infestation by the grape cane girdler, Ampeloglypter ater LeConte, near Niagara-on-the-Lake, Ontario, was reported to this laboratory on 2 June 1965. The species was identified by Mr. W. J. Brown, Taxonomic Section, Entomology Research Institute, Canada Department of Agriculture, Ottawa, from 17 aduit specimens reared or recovered from infested shoots collected in the vinevard between 11 August and 5 September 1965. There are no published records of the occurrence of this species in Canada, though two specimens collectcd at Point Pelee, Ontario, host unspecified, are in the Canadian National Collection (Brown, in lit.). The species occurs in many of the eastern United States (Brooks 1909, Whitcomb 1956).

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