scholarly journals Occurrence of Cowpea aphid-borne mosaic virus in Peanut in Brazil

Plant Disease ◽  
2000 ◽  
Vol 84 (7) ◽  
pp. 760-766 ◽  
Author(s):  
G. Pio-Ribeiro ◽  
S. S. Pappu ◽  
H. R. Pappu ◽  
G. P. Andrade ◽  
D. V. R. Reddy
Keyword(s):  
Mosaic Virus ◽  
Virus Disease ◽  
Aphis Gossypii ◽  
The United States ◽  
Northeastern Brazil ◽  
Natural Occurrence ◽  
Degenerate Primers ◽  
Cowpea Aphid ◽  
Systemic Symptoms ◽  
Leaf Symptoms

Surveys of peanut crops in northeastern Brazil since 1995 showed the occurrence of a hitherto unreported virus disease. Characteristic leaf symptoms were ring spots and blotches. The virus was seed transmitted in peanut (1/610) and cowpea (47/796). Local and systemic symptoms were observed in cowpea (cv. TVu 3433) known to be susceptible to most Cowpea aphid-borne mosaic virus (CABMV) isolates. The virus was transmitted by aphids Toxoptera citricidus and Aphis gossypii. Using degenerate primers, the 3′ terminal region of the viral genome was cloned and sequenced. Sequence analyses of the coat protein and the 3′ untranslated region indicated that the potyvirus was most closely related to CABMV isolates from South Africa, Zimbabwe, and the United States. On the basis of genome analysis, the virus was identified as CABMV. The natural occurrence of CABMV on peanut has so far not been reported. The significance of this finding especially for germ plasm exchange is discussed.

scholarly journals Sequence and Relationships of Sugarcane Mosaic and Sorghum Mosaic Virus Strains and Development of RT-PCR-Based RFLPs for Strain Discrimination

1997 ◽  
Vol 87 (9) ◽  
pp. 932-939 ◽  
Author(s):  
Z. N. Yang ◽  
T. E. Mirkov
Keyword(s):  
Mosaic Virus ◽  
Sequence Data ◽  
Virus Disease ◽  
The United States ◽  
Amino Acid Sequences ◽  
Polymorphism Analysis ◽  
Rt Pcr ◽  
Degenerate Primers ◽  
Differential Hosts ◽  
Rapid Discrimination

Sugarcane mosaic is the most widespread virus disease affecting sugarcane production. We have established a collection of seven prominent sugarcane mosaic potyvirus (SCMV) strains currently causing disease in sugarcane throughout the world and originally found in sugarcane in the United States. This collection includes SCMV strains A, B, D, and E, and the sorghum mosaic virus (SrMV) strains SCH, SCI, and SCM. These viruses were propagated on Sorghum bicolor cv. Rio and purified. Cloned cDNAs representing 2.0 kb of the 3′ termini, obtained after a reversetranscriptase-polymerase chain reaction (RT-PCR) on purified virions using an oligo(dT) primer and degenerate primers with sequences located in the NIb gene, have been sequenced for each of these strains. A comparative analysis of the deduced amino acid sequences in the NIb and coat protein genes and of the nucleotide sequences in the 3′-untranslated region, among these seven viruses and among six other members of the SCMV subgroup, confirms that there are at least four, but suggests that there are five, distinct viruses in the SCMV complex. Based on these seven new sequences and on the available sequence data for six other members of the SCMV subgroup, we have developed group-specific primers for use in RT-PCR-based restriction fragment length polymorphism analysis for rapid discrimination between strains of SCMV and SrMV. This is the first assay for differentiating strains of SCMV and SrMV that does not require interpretation of symptoms on differential hosts.

scholarly journals Diallel analysis of cowpea populations for resistance to Cowpea aphid-born mosaic virus disease (CABMV) in Burkina Faso

2017 ◽  
Vol 9 (7) ◽  
pp. 90-97
Author(s):  
Antoine Barro ◽  
Benoit Joseph Batieno ◽  
Jean-Baptiste Tignegre ◽  
Zakaria Dieni ◽  
Hamadou Sidibe ◽  
...  
Keyword(s):  
Burkina Faso ◽  
Mosaic Virus ◽  
Virus Disease ◽  
Diallel Analysis ◽  
Cowpea Aphid

scholarly journals Helleborus net necrosis virus: A New Carlavirus Associated with ‘Black Death’ of Helleborus spp.

Plant Disease ◽  
2009 ◽  
Vol 93 (4) ◽  
pp. 332-338 ◽  
Author(s):  
K. C. Eastwell ◽  
L. J. du Toit ◽  
K. L. Druffel
Keyword(s):  
United States ◽  
Mosaic Virus ◽  
The United States ◽  
Black Death ◽  
Necrosis Virus ◽  
Degenerate Primers ◽  
The Family ◽  
Net Necrosis ◽  
Curved Rod ◽  
Virus Isolates

Symptoms of ‘black death’ were observed on Helleborus spp. in each of three independent nurseries from across the United States. A new virus of the genus Carlavirus was identified in association with this disease. Symptomatic plants contained curved, rod-shaped particles averaging 800 by 17 nm, and yielded predominant bands of double-stranded (ds)RNA corresponding to approximately 9.0, 2.6, and 1.7 kbp. Amplification with degenerate primers for carlaviruses yielded a product of approximately 3,000 bp from diseased plants. Complete genomic sequences of two virus isolates were determined. Particle size, dsRNA patterns, genome organization, and sequence were consistent with members of the family Flexiviridae, genus Carlavirus. The name Helleborus net necrosis virus (HeNNV) is proposed for the virus associated with black death of Helleborus spp. in the United States. The sequence of the 3′ terminus of Helleborus mosaic virus (HeMV) (genus Carlavirus) was also determined. Nucleotide sequences of HeNNV and HeMV were only 49% identical, revealing the distinct nature of these viruses. Assays for other viruses failed to reveal a consistent association of any other virus with black death symptoms. Cucumber mosaic virus was detected in hellebore specimens both with and without distinct black death symptoms.

scholarly journals Screening of Passiflora species for reaction to Cowpea aphid-borne mosaic virus reveals an immune wild species

2009 ◽  
Vol 66 (3) ◽  
pp. 414-418 ◽  
Author(s):  
Scheila da Conceição Maciel ◽  
Daniel Hiroshi Nakano ◽  
Jorge Alberto Marques Rezende ◽  
Maria Lúcia Carneiro Vieira
Keyword(s):  
Mosaic Virus ◽  
Wild Species ◽  
Virus Disease ◽  
Passion Fruit ◽  
Mechanical Inoculation ◽  
Fruit Crops ◽  
Rt Pcr ◽  
Cowpea Aphid ◽  
Virus Isolates

Cowpea aphid-borne mosaic virus (CABMV) is a potyvirus that causes the most serious virus disease of passion fruit crops in Brazil. It is transmitted by several species of aphids in a non-persistent, non-circulative manner. The reaction of 16 species of Passiflora to infection by mechanical inoculation with four Brazilian isolates of CABMV was evaluated under greenhouse conditions. Only P. suberosa, a wild species, was resistant to infection by all virus isolates, in two independent assays. P. suberosa grafted onto infected P. edulis f. flavicarpa did not develop symptoms; neither was the virus detected by RT-PCR in the upper leaves, suggesting that this species is immune to CABMV.

scholarly journals First Report of Alternanthera mosaic virus Infection in Angelonia in the United States

Plant Disease ◽  
2008 ◽  
Vol 92 (10) ◽  
pp. 1473-1473 ◽  
Author(s):  
B. E. Lockhart ◽  
M. L. Daughtrey
Keyword(s):  
United States ◽  
Nucleotide Sequence ◽  
Mosaic Virus ◽  
Genomic Sequence ◽  
Nutrient Deficiency ◽  
Leaf Tissue ◽  
The United States ◽  
Nucleotide Sequence Analysis ◽  
Degenerate Primers ◽  
First Report

Stunting, chlorosis, and light yellow mottling resembling symptoms of nutrient deficiency were observed in angelonia (Angelonia angustifolia) in commercial production in New York. Numerous, filamentous particles 520 to 540 nm long and spherical virus particles 30 nm in diameter were observed by transmission electron microscopy (TEM) in negatively stained partially purified extracts of symptomatic Angelonia leaf tissue. Two viruses, the filamentous potexvirus Alternanthera mosaic virus (AltMV) and the spherical carmovirus Angelonia flower break virus (AnFBV) were subsequently identified on the basis of nucleotide sequence analysis of amplicons generated by reverse transcription (RT)-PCR using total RNA isolated from infected leaf tissue. A 584-bp portion of the replicase-encoding region of the AltMV genome was obtained with the degenerate primers Potex 2RC (5′-AGC ATR GNN SCR TCY TG-3′) and Potex 5 (5′-CAY CAR CAR GCM AAR GAT GA-3′) (3). Forward (AnFBV CP 1F-5′-AGC CTG GCA ATC TGC GTA CTG ATA-3′) and reverse (AnFBV CP 1R-5′-AAT ACC GCC CTC CTG TTT GGA AGT-3′) primers based on the published AnFBV genomic sequence (GenBank Accession No. NC_007733) were used to amplify a portion of the viral coat protein (CP) gene. The nucleotide sequence of the amplicon generated using the potexvirus-specific primers (GenBank Accession No. EU679362) was 99% identical to the published AltMV (GenBank Accession No. NC_007731) sequence and the nucleotide sequence of the amplicon obtained using the AnFBV CP primers was 99% identical to the published AnFBV genomic sequence (GenBank Accession No. EU679363). AnFBV occurs widely in angelonia (1) and AltMV has been identified in phlox (2). These data confirm the presence of AltMV and AnFBV in diseased angelonia plants showing stunting and nutrient deficiency-like symptoms and substantiates, to our knowledge, this first report of AltMV in angelonia in the United States. References: (1) S. Adkins et al. Phytopathology 96:460, 2006. (2) J. Hammond et al. Arch. Virol. 151:477, 2006. (3) R. A. A. van der Vlugt and M. Berendeson. Eur. J. Plant Pathol. 108:367, 2002.

scholarly journals Simple and multiple resistances to viruses in cowpea genotypes

2011 ◽  
Vol 46 (11) ◽  
pp. 1432-1438 ◽  
Author(s):  
José Albersio Araujo Lima ◽  
Ana Kelly Firmino da Silva ◽  
Maria do Livramento Aragão ◽  
Nádia Rutielly de Araújo Ferreira ◽  
Elizita Maria Teófilo
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Vigna Unguiculata ◽  
Mild Mosaic ◽  
Germplasm Bank ◽  
Positive Serology ◽  
Systemic Necrosis ◽  
Cowpea Aphid ◽  
Systemic Symptoms ◽  
New Cultivars

The objective of this work was to identify new sources of simple and multiple resistances to Cowpea severe mosaic virus (CPSMV), Cowpea aphid-borne mosaic virus (CABMV) and Cucumber mosaic virus (CMV) isolates in cowpea (Vigna unguiculata). Thirty-three genotypes from the germplasm bank of Universidade Federal do Ceará were tested as to their resistance to four CPSMV isolates, two CABMV isolates and one CMV isolate. Twenty-five days after the first virus inoculations, all inoculated plants, including the asymptomatic ones, were tested by serology. Genotypes were classified as: immune, plants without symptoms and negative serology; resistant, plants with mild mosaic and positive serology; susceptible, plants with mosaic and positive serology; and highly susceptible, plants with severe mosaic, other systemic symptoms, including systemic necrosis, and positive serology. Simple and multiple resistances to viruses were identified among the evaluated genotypes, but none of them showed multiple immunities to all isolates. Four genotypes showed immunity to all CPSMV isolates, two were immune to CABMV and two showed immunity to CMV. Eleven genotypes showed multiple resistances to two viruses, allowing for the development of new cultivars with more stable and broader resistance. Genotypes Purple Knuckle Hull-55, MNC-03-731C-21 and CNCx284-66E show resistance to CABMV, even when inoculated with CMV.

Effect of vegetable oil on the efficiency of transmission of cowpea aphid-borne mosaic virus by Aphis gossypii Glover in passion fruit plants

2021 ◽  
Author(s):  
Daniel Remor Moritz ◽  
Neemias da Silva Santos ◽  
Francis Zanini ◽  
Bruna Alana Pacini ◽  
Henrique Belmonte Petry ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Vegetable Oil ◽  
Aphis Gossypii ◽  
Passion Fruit ◽  
Aphis Gossypii Glover ◽  
Cowpea Aphid ◽  
Fruit Plants

scholarly journals Spinach latent virus Infecting Tomato in Virginia, United States

Plant Disease ◽  
2013 ◽  
Vol 97 (12) ◽  
pp. 1663-1663 ◽  
Author(s):  
J. Vargas-Asencio ◽  
H. McLane ◽  
E. Bush ◽  
K. L. Perry
Keyword(s):  
United States ◽  
Mosaic Virus ◽  
Reference Genome ◽  
Tomato Fruit ◽  
The United States ◽  
Latent Virus ◽  
Impatiens Necrotic Spot Virus ◽  
Degenerate Primers ◽  
Spot Virus ◽  
Serological Tests

Plants in a single field of commercial tomato (Solanum lycopersicum) of unidentified cultivars in Virginia in July, 2012, were observed showing stunting, leaf distortion, twisting and thickening, discoloration, and color streaking and ringspots on fruits. Serological tests were negative for Cucumber mosaic virus, Groundnut ringspot virus, Tomato spotted wilt virus, Tomato chlorotic spot virus, Impatiens necrotic spot virus, Tobacco mosaic virus, and Tomato bushy stunt virus (Agdia, Inc., Elkhart, IN). Using a membrane-based macroarray (3), hybridization was observed to 8 of 9 70-mer oligonucleotide probes of Spinach latent virus (SpLV; genus Ilarvirus, family Bromoviridae). To confirm the hybridization results, complementary DNA (cDNA) was synthesized using random hexamers and MMLV reverse transcriptase (Promega, Madison, WI), followed by PCR amplification using ilarvirus degenerate primers (4). Fragments of approximately 380 bp were amplified and directly sequenced (GenBank Accession KC_466090); a BLAST search showed a 99% identity to the SpLV RNA 2 reference genome (NC_003809). Primers for SpLV RNA1 (SpLVRNA1f-GGTGTCACCATGCAAACTGG, SpLVRNA1r-AGCTCTTCGTAATAGGCCTGC) and SpLV RNA3 (SpLVCPf-GAAGTCTTTCCCAGGTGAGCA, SpLVCPr-AGGTGGGCATATGGACTTGG) were designed and cDNA was amplified using the IQ supermix (Biorad, Hercules, CA) with thermocycling of 94°C for 4 min, 35× (94°C 45 s, 55°C 45 s, 72°C 45 s), and 72°C for 10 min. The resulting fragments of 538 bp for RNA1 (KC_466088) and 661 bp for RNA3 (KC_466089) showed 100% identity to reference genome sequences for SpLV (NC_003808 and NC_003810, respectively). To demonstrate virus transmissibility, Chenopodium quinoa plants were mechanically inoculated using tomato leaf material (same source described above) ground in 30 mM Na2HPO4 buffer, pH 7.0. Necrotic spots developed on the inoculated leaves 10 dpi. Younger, non-inoculated leaves showed yellow mottling and tested positive for SpLV by RT-PCR (two of two plants tested). The detection of SpLV is rarely reported, with only one record from the United States (2). Although SpLV is described as a latent virus, it has been found associated with tomato fruit symptoms in New Zealand (1). It is not known if the fruit ringspot and other symptoms on the Virginia samples were due to virus infection. Since SpLV is seed-transmissible and seed production takes place in different parts of the world, it has the potential to spread with germplasm and become more widespread in North America. References: (1) B. S. M. Lebas et al. Plant Dis. 91:228, 2007. (2) H. Y. Liu and J. E. Duffus. Phytopathology 76:1087, 1986. (3) K. L. Perry and X. Lu. Phytopathology 100:S100, 2010. (4) M. Untiveros, et al. J. Virol. Methods 165:97, 2010.

scholarly journals Temporal and spatial progress of Cowpea aphid-borne mosaic virus (CABMV) in passion fruit (Passiflora edulis Sims)

2021 ◽  
pp. 284-289
Author(s):  
Roger Yochiharu Kotsubo ◽  
Karina Silva dos Santos ◽  
Lucas Henrique Fantin ◽  
Vitória Carolina Antunes Chaves ◽  
João Valdecir Casaroto Filho ◽  
...  
Keyword(s):  
Spatial Pattern ◽  
Mosaic Virus ◽  
Aphis Gossypii ◽  
Management Strategies ◽  
Aphid Species ◽  
Passion Fruit ◽  
Aphis Fabae ◽  
Incidence Data ◽  
Cowpea Aphid ◽  
Aphid Vectors

Among the diseases that affect passion fruit, those causing fruit woodiness is considered the most important. Cowpea aphid-borne mosaic virus (CABMV) is an important vírus that is transmitted by several aphid species during the bite, making the use of insecticides infeasible to control these vectors. In order to understand the epidemiological behavior of the disease, this study aimed to study the temporal progress and spatial pattern of CABMV in the field. Healthy seedlings of passion fruit with 2.0 meters height were implanted in the field condition at the experimental station of the IDR-Paraná, Brazil. The evaluations were performed weekly observing the symptoms of blister, mosaic, chlorosis and crinkled leaves. For the analyses, the logistic, gompertz and monomolecular models were adjusted to CABMV incidence data in passion fruit. The spatial pattern of the disease was characterized by the dispersion index and Taylor's Power Law. The logistic model was the one that best described the progress in the incidence of the disease. The incidence progress rate of CABMV was 0.037, 0.077 and 0.060 % day-1. At the beginning of the epidemic, the pattern was random. The initial dispersion mechanism was occured through aphid vectors, como Aphis gossypii Glover, Aphis fabae Scopoli, Aphis solanella Theobald, Toxoptera citricida Kilkaldy, Uroleucon ambrosiae Thomas and Uroleucon sonchi L. infected with CABMV that starts its test bite randomly. The pattern of disease dispersion began to be added when the incidence of plants reached 10 and 7%, in the 2015/16 and 2017/18 harvests, respectively. The random spatial pattern suggested that infected aphids enter several points of the orchard and infected plants become a source for secondary infections, characterizing aggregate pattern. Thus, the eradication of alternative hosts abroad can be adopted as management strategies of CABMV

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