scholarly journals Screening of Passiflora species for reaction to Cowpea aphid-borne mosaic virus reveals an immune wild species

2009 ◽  
Vol 66 (3) ◽  
pp. 414-418 ◽  
Author(s):  
Scheila da Conceição Maciel ◽  
Daniel Hiroshi Nakano ◽  
Jorge Alberto Marques Rezende ◽  
Maria Lúcia Carneiro Vieira
Keyword(s):  
Mosaic Virus ◽  
Wild Species ◽  
Virus Disease ◽  
Passion Fruit ◽  
Mechanical Inoculation ◽  
Fruit Crops ◽  
Rt Pcr ◽  
Cowpea Aphid ◽  
Virus Isolates

Cowpea aphid-borne mosaic virus (CABMV) is a potyvirus that causes the most serious virus disease of passion fruit crops in Brazil. It is transmitted by several species of aphids in a non-persistent, non-circulative manner. The reaction of 16 species of Passiflora to infection by mechanical inoculation with four Brazilian isolates of CABMV was evaluated under greenhouse conditions. Only P. suberosa, a wild species, was resistant to infection by all virus isolates, in two independent assays. P. suberosa grafted onto infected P. edulis f. flavicarpa did not develop symptoms; neither was the virus detected by RT-PCR in the upper leaves, suggesting that this species is immune to CABMV.

scholarly journals Cowpea aphid-borne mosaic virus (CABMV) in Passiflora by qPCR analysis reveals asymptomatic plants with viruses and new species with immunity

2021 ◽  
Author(s):  
Zanon Santana Gonçalves ◽  
Onildo Nunes de Jesus ◽  
Lucas Kennedy Silva Lima ◽  
Ronan Xavier Corrêa
Keyword(s):  
New Species ◽  
Virus Infection ◽  
Mosaic Virus ◽  
Disease Index ◽  
Passion Fruit ◽  
Rt Pcr ◽  
Qpcr Analysis ◽  
Resistant Varieties ◽  
Cowpea Aphid ◽  
Temporal Progress

Abstract The passion fruit woodiness disease (Cowpea aphid-borne mosaic virus – CABMV) causes socioeconomic problems for Brazilian passion fruit crop. Understanding the temporal progress of the disease and identifying resistance sources to CABMV are essential steps to develop resistant varieties. The objective of the study was to evaluate temporal progress of passion fruit woodiness disease, identify Passiflora genotypes with CABMV resistance and to detect virus infection in asymptomatic plants by qPCR. The experiment was conducted in a greenhouse using 128 genotypes belonging to 12 species and three hybrids (inter and intraspecific) of Passiflora evaluated in five periods after inoculation. The symptoms severity was quantified from the disease index (DI%). The CABMV infection in symptomatic plants was confirmed by RT-PCR and in asymptomatic plants by qPCR. Progress rates and disease severity were lower in the species P. cincinnata, P. gibertii, P. miersii e P. mucronata compared to P. edulis, P. alata, Passiflora sp. and hybrids. Of the evaluated genotypes, 20.31% were resistant, with emphasis on the accessions of P. suberosa, P. malacophylla, P. setacea, P. pohlii e P. bahiensis that did not show symptoms of virus. The absence of symptoms does not imply immunity of plants to the virus, as the qPCR analysis confirmed infection by the virus in asymptomatic plants of P. cincinnata, P. gibertii, P. miersii, P. mucronata, P. setacea, P. malacophylla e P. suberosa. Even after four inoculations, the virus was not detected by qPCR in the upper leaves in plants of P. pohlii and P. bahiensis indicating that these species are immune to CABMV.

scholarly journals Interaction between Cowpea Aphid-Borne Mosaic Virus Isolates and Its Effect on Passion Fruit Woodiness Disease on Passiflora edulis Sims and Passiflora ligularis Juss

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Lucy J. Kiptui ◽  
Faith J. Toroitich ◽  
Dora C. Kilalo ◽  
Meshack Obonyo
Keyword(s):  
Mosaic Virus ◽  
Elisa Test ◽  
Synergistic Interaction ◽  
Passion Fruit ◽  
Economic Losses ◽  
Passiflora Edulis ◽  
Cowpea Aphid ◽  
Virus Isolates ◽  
Screen House ◽  
Viral Isolates

In East Africa, passion fruit woodiness disease is caused by potyviruses, among which are Cowpea Aphid-Borne Mosaic Virus (CABMV) and Uganda Passiflora Virus (UPV). Previous studies suggest that synergistic interaction of viruses causes mild or severe outcomes of the disease. However, mixed infections of these viruses have not been documented. The aim of this study was to determine the effects of the interaction between two CABMV isolates causing passion fruit woodiness disease in Kenya. Healthy plants were mechanically inoculated at six-leaf stage. Four treatments were used: CABMV isolate 1, CABMV isolate 2, mixture of the two isolates, and noninoculated control. The test plants were maintained in a screen house, and data on symptom severity and rate of disease development were recorded. ELISA test was conducted to confirm virus presence and distribution. The results indicate that the CABMV isolates used are systemic. There were various differences in disease progression, which was faster in the sweet passion variety than in the purple variety. However, the latter had a higher disease severity. Coinfected plants expressed severe symptoms compared to the singly infected ones, indicating synergistic interaction between the viral isolates. This implies that coinfection has adverse impact resulting in higher economic losses.

scholarly journals Yellow passion fruit reaction to Xanthomonas axonopodis pv. passiflorae and to Cowpea aphid-borne mosaic virus

2018 ◽  
Vol 18 (4) ◽  
pp. 349-356
Author(s):  
Anne Pinheiro Costa ◽  
Isadora Nogueira ◽  
José Ricardo Peixoto ◽  
Michelle de Souza Vilela ◽  
Luiz Eduardo Bassay Blum ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Passion Fruit ◽  
Xanthomonas Axonopodis ◽  
Cowpea Aphid ◽  
Yellow Passion Fruit

scholarly journals Transcriptome Sequencing Reveals a Complete Genome Sequence of Cowpea Aphid-Borne Mosaic Virus from Passion Fruit in Kenya

2019 ◽  
Vol 8 (2) ◽  
Author(s):  
F. Munguti ◽  
S. Maina ◽  
E. N. Nyaboga ◽  
D. Kilalo ◽  
E. Kimani ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Transcriptome Sequencing ◽  
Passion Fruit ◽  
Rna Seq ◽  
Cowpea Aphid

Analysis of transcriptome sequencing (RNA-Seq) data revealed a complete Cowpea aphid-borne mosaic virus (CABMV) genome from virus-infected passion fruit in Kenya. We compared it with six complete CABMV genomes, one each from Zimbabwe and Uganda and two each from Brazil and India.

scholarly journals Diallel analysis of cowpea populations for resistance to Cowpea aphid-born mosaic virus disease (CABMV) in Burkina Faso

2017 ◽  
Vol 9 (7) ◽  
pp. 90-97
Author(s):  
Antoine Barro ◽  
Benoit Joseph Batieno ◽  
Jean-Baptiste Tignegre ◽  
Zakaria Dieni ◽  
Hamadou Sidibe ◽  
...  
Keyword(s):  
Burkina Faso ◽  
Mosaic Virus ◽  
Virus Disease ◽  
Diallel Analysis ◽  
Cowpea Aphid

Yellow passion fruit genotypes reaction to woodiness disease (Cowpea aphid-borne mosaic virus) in field conditions

2020 ◽  
pp. 427-432
Author(s):  
G.S. Miranda ◽  
O.K. Yamanishi ◽  
J.R. Peixoto ◽  
M. de S. Vilella ◽  
M. de C. Pires ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Passion Fruit ◽  
Field Conditions ◽  
Cowpea Aphid ◽  
Yellow Passion Fruit

Genetic transformation of sweet passion fruit (Passiflora alata) and reactions of the transgenic plants to Cowpea aphid borne mosaic virus

2015 ◽  
Vol 143 (4) ◽  
pp. 813-821 ◽  
Author(s):  
Marcelo Favareto Correa ◽  
Ana Paula Chiaverini Pinto ◽  
Jorge Alberto Marques Rezende ◽  
Ricardo Harakava ◽  
Beatriz Madalena Januzzi Mendes
Keyword(s):  
Transgenic Plants ◽  
Genetic Transformation ◽  
Mosaic Virus ◽  
Passion Fruit ◽  
Cowpea Aphid

scholarly journals Occurrence of Cowpea aphid-borne mosaic virus in Peanut in Brazil

Plant Disease ◽  
2000 ◽  
Vol 84 (7) ◽  
pp. 760-766 ◽  
Author(s):  
G. Pio-Ribeiro ◽  
S. S. Pappu ◽  
H. R. Pappu ◽  
G. P. Andrade ◽  
D. V. R. Reddy
Keyword(s):  
Mosaic Virus ◽  
Virus Disease ◽  
Aphis Gossypii ◽  
The United States ◽  
Northeastern Brazil ◽  
Natural Occurrence ◽  
Degenerate Primers ◽  
Cowpea Aphid ◽  
Systemic Symptoms ◽  
Leaf Symptoms

Surveys of peanut crops in northeastern Brazil since 1995 showed the occurrence of a hitherto unreported virus disease. Characteristic leaf symptoms were ring spots and blotches. The virus was seed transmitted in peanut (1/610) and cowpea (47/796). Local and systemic symptoms were observed in cowpea (cv. TVu 3433) known to be susceptible to most Cowpea aphid-borne mosaic virus (CABMV) isolates. The virus was transmitted by aphids Toxoptera citricidus and Aphis gossypii. Using degenerate primers, the 3′ terminal region of the viral genome was cloned and sequenced. Sequence analyses of the coat protein and the 3′ untranslated region indicated that the potyvirus was most closely related to CABMV isolates from South Africa, Zimbabwe, and the United States. On the basis of genome analysis, the virus was identified as CABMV. The natural occurrence of CABMV on peanut has so far not been reported. The significance of this finding especially for germ plasm exchange is discussed.

Sign in / Sign up

Export Citation Format

Share Document