scholarly journals Mapping of crown rust (Puccinia coronata f. sp. avenae) resistance gene Pc54 and a novel quantitative trait locus effective against powdery mildew (Blumeria graminis f. sp. avenae) in the oat (Avena sativa) line Pc54

2022 ◽  
Author(s):  
Belayneh Admassu Yimer ◽  
Kathy Esvelt Klos ◽  
Irene Griffiths ◽  
Alexander Cowan ◽  
Catherine Howarth
Keyword(s):  
Molecular Markers ◽  
Powdery Mildew ◽  
Resistance Gene ◽  
Interval Mapping ◽  
Crown Rust ◽  
The Novel ◽  
Unknown Gene ◽  
Trait Locus ◽  
Separate Identity ◽  
Genomic Locations

The Pc54 oat line carries the crown rust resistance gene ‘Pc54’ and an unknown gene effective against powdery mildew. In this study two recombinant inbred line populations were developed to identify the genomic locations of the two genes and producing lists of molecular markers with a potential for marker assisted selection. The RILs and parents were phenotyped for crown rust and powdery mildew in a controlled environment. They were also genotyped using the 6K Illumina Infinium iSelect oat SNP chip. Multiple interval mapping placed Pc54 on the linkage group Mrg02 (chromosome 7D) and the novel powdery mildew QTL ‘QPm.18’ on Mrg18 (chromosome 1A) both in the mapping and validating population. A total of nine and 31 significant molecular markers were identified linked with the Pc54 gene and QPm.18, respectively. Reactions to crown rust inoculations have justified separate identity of Pc54 from other genes and QTL that have previously been reported on Mrg02 except for ’qPCRFd’. Pm3 is the only powdery mildew resistance gene previously mapped on Mrg18. However, the pm3 differential line, Mostyn was susceptible to the powdery mildew race used in this study suggesting that Pm3 and QPm.18 are different genes. Determining the chromosomal locations of Pc54 and QPm.18 is helpful for better understanding the molecular mechanism of resistance to crown rust and powdery mildew in oats. Furthermore, SNPs and SSRs that are closely linked with the genes could be valuable for developing PCR based molecular markers and facilitating the utilization of these genes in oat breeding programs.

scholarly journals Inheritance and Identification of a Major Quantitative Trait Locus (QTL) that Confers Resistance to Meloidogyne incognita and a Novel QTL for Plant Height in Sweet Sorghum

2015 ◽  
Vol 105 (12) ◽  
pp. 1522-1528 ◽  
Author(s):  
Karen R. Harris-Shultz ◽  
Richard F. Davis ◽  
Joseph E. Knoll ◽  
William Anderson ◽  
Hongliang Wang
Keyword(s):  
Quantitative Trait Locus ◽  
Resistance Gene ◽  
Meloidogyne Incognita ◽  
Quantitative Trait ◽  
Sweet Sorghum ◽  
Major Quantitative Trait Locus ◽  
Interval Mapping ◽  
Chromosome 3 ◽  
Trait Locus ◽  
Stalk Weight

Southern root-knot nematodes (Meloidogyne incognita) are a pest on many economically important row crop and vegetable species and management relies on chemicals, plant resistance, and cultural practices such as crop rotation. Little is known about the inheritance of resistance to M. incognita or the genomic regions associated with resistance in sorghum (Sorghum bicolor). In this study, an F2 population (n = 130) was developed between the resistant sweet sorghum cultivar ‘Honey Drip’ and the susceptible sweet cultivar ‘Collier’. Each F2 plant was phenotyped for stalk weight, height, juice Brix, root weight, total eggs, and eggs per gram of root. Strong correlations were observed between eggs per gram of root and total eggs, height and stalk weight, and between two measurements of Brix. Genotyping-by-sequencing was used to generate single nucleotide polymorphism markers. The G-Model, single marker analysis, interval mapping, and composite interval mapping were used to identify a major quantitative trait locus (QTL) on chromosome 3 for total eggs and eggs per gram of root. Furthermore, a new QTL for plant height was also discovered on chromosome 3. Simple sequence repeat markers were developed in the total eggs and eggs per gram of root QTL region and the markers flanking the resistance gene are 4.7 and 2.4 cM away. These markers can be utilized to move the southern root-knot nematode resistance gene from Honey Drip to any sorghum line.

Chromosomal location of a Triticum timopheevii - derived powdery mildew resistance gene transferred to common wheat

Genome ◽  
2000 ◽  
Vol 43 (2) ◽  
pp. 377-381 ◽  
Author(s):  
K Järve ◽  
H O Peusha ◽  
J Tsymbalova ◽  
S Tamm ◽  
K M Devos ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Resistance Gene ◽  
Common Wheat ◽  
Powdery Mildew Resistance ◽  
Molecular Data ◽  
Monosomic Analysis ◽  
The Novel ◽  
Triticum Timopheevii ◽  
Powdery Mildew Resistance Gene ◽  
Mildew Resistance

A dominant powdery mildew resistance gene introduced from Triticum timopheevii in line 146-155-T of common wheat, Triticum aestivum, was located on chromosome 6B by monosomic analysis. Restriction fragment length polymorphism (RFLP) and microsatellite analyses detected the presence of a T. timopheevii segment, translocated to chromosome 6B, with breakpoints between the loci Xpsr8/Xpsr964 on 6BS and Xpsr154/Xpsr546 on 6BL. The novel powdery mildew resistance gene, which has been designated Pm27, was shown to cosegregate with the microsatellite locus Xpsp3131, which is located on the introgressed T. timopheevii segment. The molecular data confirm the location of Pm27 on the translocated 6B chromosome. Key words: monosomic analysis, RFLP, microsatellites, Pm27.

Genetic map of the powdery mildew resistance gene in soybean PI 243540

Genome ◽  
2010 ◽  
Vol 53 (5) ◽  
pp. 400-405 ◽  
Author(s):  
Sung-Taeg Kang ◽  
M.A. Rouf Mian
Keyword(s):  
Molecular Markers ◽  
Powdery Mildew ◽  
Resistance Gene ◽  
Powdery Mildew Resistance ◽  
Dominant Gene ◽  
Common Disease ◽  
Single Dominant Gene ◽  
Powdery Mildew Resistance Gene ◽  
Chromosome 16 ◽  
Mildew Resistance

Powdery mildew (caused by Microsphaera diffusa Cooke & Peck) is a common disease of soybean in many soybean-growing regions of the world and under greenhouse conditions. The previously reported Rmd locus of soybean for resistance to powdery mildew was mapped on soybean molecular linkage group J (chromosome 16). We have discovered a single dominant gene in PI 243540 that provides season-long resistance to powdery mildew. The objective of this study was to map the powdery mildew resistance gene in PI 243540 with PCR-based molecular markers. One hundred eighty-four F2 plants and their F2:3 families from a cross between the powdery mildew susceptible cultivar ‘Wyandot’ and PI 243540 were screened with M. diffusa in greenhouses. Bulked segregant analysis (BSA) with SSR markers was used to identify the tentative genomic location of the gene. The BSA localized the gene to a genomic region in soybean chromosome 16. A linkage map with seven SSR and six SNP markers flanking the gene was constructed. We positioned the gene between SSR marker Sat_224 and SNP marker BARC-021875-04228 at distances of 9.6 and 1.3 cM from the markers, respectively. The map position of the gene was slightly different from previously reported map positions of the only known Rmd locus. We have mapped a single dominant gene, tentatively called Rmd_PI243540, near the previously known Rmd locus on chromosome 16. The molecular markers flanking the gene will be useful for marker-assisted selection of this gene.

scholarly journals Discovery of powdery mildew resistance gene candidates from Aegilops biuncialis chromosome 2Mb based on transcriptome sequencing

2019 ◽  
Author(s):  
Huanhuan Li ◽  
Zhenjie Dong ◽  
Chao Ma ◽  
Xiubin Tian ◽  
Zhiguo Xiang ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Disease Resistance ◽  
Powdery Mildew ◽  
Resistance Gene ◽  
Powdery Mildew Resistance ◽  
Disease Resistance Gene ◽  
R Genes ◽  
Powdery Mildew Resistance Gene ◽  
Mildew Resistance ◽  
Resistance Gene Candidates

AbstractPowdery mildew is one of the most widespread diseases of wheat. Breeding resistant varieties by utilization of resistance genes is considered as the most economic and effective method of controlling this disease. Previous study showed that the gene(s) at 2Mb in Chinese Spring (CS)-Aegilops biuncialis 2Mb disomic addition line TA7733 conferred high resistance to powdery mildew. In this study, 15 Bgt isolates prevalent in different regions of China were used to further test the resistance spectrum of TA7733. As a result, TA7733 was high resistance to all tested isolates, indicating that the gene(s) on chromosome 2Mb was broad-spectrum powdery mildew resistance. In order to mine resistance gene candidates and develop 2Mb-specific molecular markers to assist the transfer resistance gene(s) at chromosome 2Mb, RNA-seq of TA7733 and CS was conducted before and after Bgt-infection, generating a total of 158,953 unigenes. Of which, 7,278 unigenes were TA7733-specific which were not expressed in CS, and 295 out of these 7,278 unigenes were annotated as R genes. Based on Blastn against with CS Ref Seq v1.0, 61 R genes were further mapped to homoeologous group 2. Analysis of R gene-specific molecular markers designed from R gene sequences verified 40 out of 61 R genes to be 2Mb specific. Annotation of these 40 R genes showed most genes encoded nucleotide binding leucine rich repeat (NLR) protein, being most likely resistance gene candidates. The broad-spectrum powdery mildew resistance gene(s), disease resistance gene candidates, and functional molecular markers of 2Mb-specific in present study will not only lay foundations for transferring disease resistance gene(s) from 2Mb to common wheat by inducing CS-Ae. biuncialis homoeologous recombination, but also provide useful candidates for isolating and cloning resistance gene(s) and dissecting molecular and genetic mechanisms of disease resistance from 2Mb.

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