scholarly journals Analysis of Differentially Expressed Rice Genes Reveals the ATP-Binding Cassette (ABC) Transporters as a Candidate Gene Against the Sheath Blight Pathogen, Rhizoctonia solani

2021 ◽  
Author(s):  
Youngjae Oh ◽  
Seonghee Lee ◽  
Renee Arielle Rioux ◽  
Pratibha Singh ◽  
Melissa H Jia ◽  
...  
Keyword(s):  
Gene Family ◽  
Rhizoctonia Solani ◽  
Cdna Library ◽  
Abc Transporters ◽  
Sheath Blight ◽  
Differentially Expressed ◽  
Blight Resistance ◽  
Atp Binding ◽  
Resistance Qtl ◽  
Sheath Blight Resistance

Sheath blight is a serious rice disease worldwide and genes involved in resistance remain unclear. In the present study, a virulent field isolate of Rhizoctonia solani was used to inoculate detached leaves of a sheath blight resistant rice cultivar ‘Jasmine 85’, a suppression subtractive cDNA library was constructed using RNA isolated 16 hours post inoculation (hpi), and differentially expressed genes were identified from the cDNA library. A total of 159 uniquely expressed sequence tags were identified, including 105 from rice with enrichment in categories related to cellular response, molecular signaling and host defense. Coupled with gene expression studies by DNA microarray, 27 highly induced genes involved in signal transduction and defense responses were identified within 16 hpi. Three members of the ABC transporter gene family (OsABC1, OsABC9 and OsABC12) encoding pleiotropic drug resistance (PDR)-like ATP binding cassette (ABC) transporters were mapped to different sheath blight resistance QTL and their differential expressions were validated. Three high-resolution melting (HRM) markers were developed from these ABC gene family members to distinguish alleles between sheath blight susceptible cultivar ‘Lemont’ and resistant cultivar ‘Jasmine 85’. Association of sheath blight resistance to these HRM markers was examined in 77 recombinant inbred lines derived from the cross between ‘Jasmine 85’ and “Lemont”. The OsABC9 gene located in a major sheath blight resistance QTL qShB9-2 showed a major contribution to sheath blight resistance. These results are useful for marker assisted section and functional validation of the ABC genes in sheath blight disease resistance.

Rice Pyramiding Breeding Using Sheath Blight Resistance QTL qSB-9TQ and Stripe Disease Resistance Gene Stv-bi

2013 ◽  
Vol 38 (7) ◽  
pp. 1178-1186 ◽  
Author(s):  
Zong-Xiang CHEN ◽  
Shi-Min ZUO ◽  
Ya-Fang ZHANG ◽  
Jun-Kai ZHU ◽  
Long-Ping WANG ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Resistance Gene ◽  
Sheath Blight ◽  
Blight Resistance ◽  
Disease Resistance Gene ◽  
Resistance Qtl ◽  
Sheath Blight Resistance

The way to a more precise sheath blight resistance QTL in rice

Euphytica ◽  
2014 ◽  
Vol 203 (1) ◽  
pp. 33-45 ◽  
Author(s):  
Yuxiang Zeng ◽  
Zhijuan Ji ◽  
Changdeng Yang
Keyword(s):  
Sheath Blight ◽  
Blight Resistance ◽  
Resistance Qtl ◽  
Sheath Blight Resistance ◽  
The Way

scholarly journals Strategies to Manage Rice Sheath Blight: Lessons from Interactions between Rice and Rhizoctonia solani

Rice ◽  
2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Dayong Li ◽  
Shuai Li ◽  
Songhong Wei ◽  
Wenxian Sun
Keyword(s):  
Rhizoctonia Solani ◽  
Anastomosis Group ◽  
Sheath Blight ◽  
Phytopathogenic Fungus ◽  
Blight Resistance ◽  
Rice Sheath Blight ◽  
Resistance Qtls ◽  
Necrotrophic Pathogen ◽  
Worldwide Distribution ◽  
Sheath Blight Resistance

AbstractRhizoctonia solani is an important phytopathogenic fungus with a wide host range and worldwide distribution. The anastomosis group AG1 IA of R. solani has been identified as the predominant causal agent of rice sheath blight, one of the most devastating diseases of crop plants. As a necrotrophic pathogen, R. solani exhibits many characteristics different from biotrophic and hemi-biotrophic pathogens during co-evolutionary interaction with host plants. Various types of secondary metabolites, carbohydrate-active enzymes, secreted proteins and effectors have been revealed to be essential pathogenicity factors in R. solani. Meanwhile, reactive oxygen species, phytohormone signaling, transcription factors and many other defense-associated genes have been identified to contribute to sheath blight resistance in rice. Here, we summarize the recent advances in studies on molecular interactions between rice and R. solani. Based on knowledge of rice-R. solani interactions and sheath blight resistance QTLs, multiple effective strategies have been developed to generate rice cultivars with enhanced sheath blight resistance.

scholarly journals In Silico Genome-Wide Analysis of the ATP-Binding Cassette Transporter Gene Family in Soybean (Glycine max L.) and Their Expression Profiling

2019 ◽  
Vol 2019 ◽  
pp. 1-14 ◽  
Author(s):  
Awdhesh Kumar Mishra ◽  
Jinhee Choi ◽  
Muhammad Fazle Rabbee ◽  
Kwang-Hyun Baek
Keyword(s):  
Gene Family ◽  
Abc Transporter ◽  
Abc Transporters ◽  
In Silico ◽  
Gene Families ◽  
Protein Domain ◽  
Atp Binding ◽  
Transporter Gene ◽  
Genome Wide ◽  
Atp Binding Cassette

ATP-binding cassette (ABC) transporters constitute one of the largest gene families in all living organisms, most of which mediate transport across biological membranes by hydrolyzing ATP. However, detailed studies of ABC transporter genes in the important oil crop, soybean, are still lacking. In the present study, we carried out genome-wide identification and phylogenetic and transcriptional analyses of the ABC gene family in G. max. A total of 261 G. max ABC (GmABCs) genes were identified and unevenly localized onto 20 chromosomes. Referring to protein-domain orientation and phylogeny, the GmABC family could be classified into eight (ABCA-ABCG and ABCI) subfamilies and ABCG were the most abundantly present. Further, investigation of whole genome duplication (WGD) signifies the role of segmental duplication in the expansion of the ABC transporter gene family in soybean. The Ka/Ks ratio indicates that several duplicated genes are governed by intense purifying selection during evolution. In addition, in silico expression analysis based on RNA-sequence using publicly available database revealed that ABC transporters are differentially expressed in tissues and developmental stages and in dehydration. Overall, we provide an extensive overview of the GmABC transporter gene family and it promises the primary basis for the study in development and response to dehydration tolerance.

Tissue-specific expression of Arabidopsis NPR1 gene in rice for sheath blight resistance without compromising phenotypic cost

Plant Science ◽  
2016 ◽  
Vol 250 ◽  
pp. 105-114 ◽  
Author(s):  
Kutubuddin Ali Molla ◽  
Subhasis Karmakar ◽  
Palas Kumar Chanda ◽  
Sailendra Nath Sarkar ◽  
Swapan Kumar Datta ◽  
...  
Keyword(s):  
Sheath Blight ◽  
Blight Resistance ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression ◽  
Sheath Blight Resistance ◽  
Npr1 Gene

Sheath-blight resistance QTLS in japonica rice germplasm

Euphytica ◽  
2011 ◽  
Vol 184 (1) ◽  
pp. 23-34 ◽  
Author(s):  
J. C. Nelson ◽  
J. H. Oard ◽  
D. Groth ◽  
H. S. Utomo ◽  
Y. Jia ◽  
...  
Keyword(s):  
Sheath Blight ◽  
Blight Resistance ◽  
Japonica Rice ◽  
Resistance Qtls ◽  
Rice Germplasm ◽  
Sheath Blight Resistance
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