Engineering Arabidopsis – Specific Nonhost Resistance Mechanism in Rice for Durable and Broad Spectrum Sheath Blight Resistance against Rhizoctonia solani

Sheath blight is a serious rice disease worldwide and genes involved in resistance remain unclear. In the present study, a virulent field isolate of Rhizoctonia solani was used to inoculate detached leaves of a sheath blight resistant rice cultivar ‘Jasmine 85’, a suppression subtractive cDNA library was constructed using RNA isolated 16 hours post inoculation (hpi), and differentially expressed genes were identified from the cDNA library. A total of 159 uniquely expressed sequence tags were identified, including 105 from rice with enrichment in categories related to cellular response, molecular signaling and host defense. Coupled with gene expression studies by DNA microarray, 27 highly induced genes involved in signal transduction and defense responses were identified within 16 hpi. Three members of the ABC transporter gene family (OsABC1, OsABC9 and OsABC12) encoding pleiotropic drug resistance (PDR)-like ATP binding cassette (ABC) transporters were mapped to different sheath blight resistance QTL and their differential expressions were validated. Three high-resolution melting (HRM) markers were developed from these ABC gene family members to distinguish alleles between sheath blight susceptible cultivar ‘Lemont’ and resistant cultivar ‘Jasmine 85’. Association of sheath blight resistance to these HRM markers was examined in 77 recombinant inbred lines derived from the cross between ‘Jasmine 85’ and “Lemont”. The OsABC9 gene located in a major sheath blight resistance QTL qShB9-2 showed a major contribution to sheath blight resistance. These results are useful for marker assisted section and functional validation of the ABC genes in sheath blight disease resistance.

Download Full-text

Morphological characterization and screening for sheath blight resistance using Indian isolates of Rhizoctonia solani AG1IA

Indian Phytopathology ◽

10.1007/s42360-018-0103-2 ◽

2019 ◽

Vol 72 (1) ◽

pp. 107-124 ◽

Cited By ~ 2

Author(s):

Sanjay K. Goswami ◽

Vineeta Singh ◽

P. L. Kashyap ◽

P. K. Singh

Keyword(s):

Rhizoctonia Solani ◽

Morphological Characterization ◽

Sheath Blight ◽

Blight Resistance ◽

Indian Isolates ◽

Sheath Blight Resistance

Download Full-text

Strategies to Manage Rice Sheath Blight: Lessons from Interactions between Rice and Rhizoctonia solani

Rice ◽

10.1186/s12284-021-00466-z ◽

2021 ◽

Vol 14 (1) ◽

Author(s):

Dayong Li ◽

Shuai Li ◽

Songhong Wei ◽

Wenxian Sun

Keyword(s):

Rhizoctonia Solani ◽

Anastomosis Group ◽

Sheath Blight ◽

Phytopathogenic Fungus ◽

Blight Resistance ◽

Rice Sheath Blight ◽

Resistance Qtls ◽

Necrotrophic Pathogen ◽

Worldwide Distribution ◽

Sheath Blight Resistance

AbstractRhizoctonia solani is an important phytopathogenic fungus with a wide host range and worldwide distribution. The anastomosis group AG1 IA of R. solani has been identified as the predominant causal agent of rice sheath blight, one of the most devastating diseases of crop plants. As a necrotrophic pathogen, R. solani exhibits many characteristics different from biotrophic and hemi-biotrophic pathogens during co-evolutionary interaction with host plants. Various types of secondary metabolites, carbohydrate-active enzymes, secreted proteins and effectors have been revealed to be essential pathogenicity factors in R. solani. Meanwhile, reactive oxygen species, phytohormone signaling, transcription factors and many other defense-associated genes have been identified to contribute to sheath blight resistance in rice. Here, we summarize the recent advances in studies on molecular interactions between rice and R. solani. Based on knowledge of rice-R. solani interactions and sheath blight resistance QTLs, multiple effective strategies have been developed to generate rice cultivars with enhanced sheath blight resistance.

Download Full-text