The stimulation of Na,K,Cl cotransport and of system A for neutral amino acid transport is a mechanism for cell volume increase during the cell cycle

1996 ◽  
Vol 10 (8) ◽  
pp. 920-926 ◽  
Author(s):  
Ovidio Bussolati ◽  
Jacopo Uggeri ◽  
Silvana Belletti ◽  
Valeria Dall'Asta ◽  
Gian C. Gazzola
Keyword(s):  
Cell Cycle ◽  
Amino Acid ◽  
Cell Volume ◽  
Amino Acid Transport ◽  
Neutral Amino Acid ◽  
Acid Transport ◽  
Volume Increase ◽  
System A ◽  
Stimulation Of

scholarly journals The role of system A for neutral amino acid transport in the regulation of cell volume

2001 ◽  
Vol 18 (1) ◽  
pp. 27-38 ◽  
Author(s):  
Ovidio Bussolati ◽  
Valeria Dall'Asta ◽  
Renata Franchi-Gazzola ◽  
Roberto Sala ◽  
Bianca Maria Rotoli ◽  
...  
Keyword(s):  
Amino Acid ◽  
Cell Volume ◽  
Amino Acid Transport ◽  
Neutral Amino Acid ◽  
Acid Transport ◽  
System A

scholarly journals Electrogenic Na+-dependentl-alanine transport in the lizard duodenum. Involvement of systems A and ASC

2001 ◽  
Vol 280 (3) ◽  
pp. R612-R622
Author(s):  
Virtudes Medina ◽  
Antonio Lorenzo ◽  
Mario Dı́az
Keyword(s):  
Amino Acid ◽  
Amino Acid Transport ◽  
Short Circuit ◽  
Duodenal Mucosa ◽  
Neutral Amino Acid ◽  
Acid Transport ◽  
Transport Activity ◽  
System A ◽  
Alanine Transport ◽  
Gallotia Galloti

l-Alanine transport across the isolated duodenal mucosa of the lizard Gallotia galloti has been studied in Ussing chambers under short-circuit conditions. Net l-alanine fluxes, transepithelial potential difference (PD), and short-circuit current ( Isc) showed concentration-dependent relationships. Na+-dependent l-alanine transport was substantially inhibited by the analog α-methyl aminoisobutyric acid (MeAIB). Likewise, MeAIB fluxes were completely inhibited byl-alanine, indicating the presence of system A for neutral amino acid transport. System A transport activity was electrogenic and exhibited hyperbolic relationships for net MeAIB fluxes, PD, and Isc, which displayed similar apparent K m values. Na+-dependentl-alanine transport, but not MeAIB transport, was partially inhibited by l-serine and l-cysteine, indicating the participation of system ASC. This transport activity represents the major pathway for l-alanine absorption and seemed to operate in an electroneutral mode with a negligible contribution to the l-alanine-induced electrogenicity. It is concluded from the present study that the active Na+-dependent l-alanine transport across the isolated duodenal mucosa of Gallotia galloti results from the independent activity of systems A and ASC for neutral amino acid transport.

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