scholarly journals Expression of Vascular Endothelial Growth Factor (VEGF) Isoforms VEGFxxxb in Normal Human Renal Development

2006 ◽  
Vol 20 (4) ◽  
Author(s):  
Heather Samantha Bevan ◽  
Nynke N.M.S van den Akker ◽  
Japke A.E Polman ◽  
Steve J Harper ◽  
Adriana C Gittenberger‐de Groot ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Renal Development ◽  
Vascular Endothelial ◽  
Normal Human ◽  
Endothelial Growth Factor ◽  
Vegf Isoforms

scholarly journals The vascular endothelial growth factor (VEGF) isoforms: differential deposition into the subepithelial extracellular matrix and bioactivity of extracellular matrix-bound VEGF.

1993 ◽  
Vol 4 (12) ◽  
pp. 1317-1326 ◽  
Author(s):  
J E Park ◽  
G A Keller ◽  
N Ferrara
Keyword(s):  
Extracellular Matrix ◽  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Vascular Endothelial ◽  
Vegf Mrna ◽  
Angiogenic Potential ◽  
Molecular Masses ◽  
Matrix Bound ◽  
Endothelial Growth Factor ◽  
Vegf Isoforms

Vascular endothelial growth factor (VEGF)mRNA undergoes alternative splicing events that generate four different homodimeric isoforms, VEGF121, VEGF165, VEGF189, or VEGF206. VEGF121 is a nonheparin-binding acidic protein, which is freely diffusible. The longer forms, VEGF189 or VEGF206, are highly basic proteins tightly bound to extracellular heparin-containing proteoglycans. VEGF165 has intermediate properties. To determine the localization of VEGF isoforms, transfected human embryonic kidney CEN4 cells expressing VEGF165, VEGF189, or VEGF206 were stained by immunofluorescence with a specific monoclonal antibody. The staining was found in patches and streaks suggestive of extracellular matrix (ECM). VEGF165 was observed largely in Golgi apparatus-like structures. Immunogold labeling of cells expressing VEGF189 or VEGF206 revealed that the staining was localized to the subepithelial ECM. VEGF associated with the ECM was bioactive, because endothelial cells cultured on ECM derived from cells expressing VEGF189 or VEGF206 were markedly stimulated to proliferate. In addition, ECM-bound VEGF can be released into a soluble and bioactive form by heparin or plasmin. ECM-bound VEGF189 and VEGF206 have molecular masses consistent with the intact polypeptides. The ECM may represent an important source of VEGF and angiogenic potential.

Cell migration and growth induced by photo-immobilised vascular endothelial growth factor (VEGF) isoforms

2019 ◽  
Vol 7 (27) ◽  
pp. 4272-4279 ◽  
Author(s):  
Xueli Ren ◽  
Jun Akimoto ◽  
Hideyuki Miyatake ◽  
Seiichi Tada ◽  
Liping Zhu ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Cell Migration ◽  
Growth Factor ◽  
Vascular Endothelial ◽  
Cell Migration And Proliferation ◽  
Endothelial Growth Factor ◽  
Vegf Isoforms

VEGF isoforms immobilised by photo-reactive gelatin (AzPhe-gelatin) enhance cell migration and proliferation.

Effect of Tibolone and its metabolites on vascular endothelial growth factor (VEGF) isoforms 121 and 165 in ishikawa cells

2002 ◽  
Vol 78 ◽  
pp. S159-S160
Author(s):  
Sebastian Mirkin ◽  
Susan Leslie ◽  
Michelle Billeter ◽  
Mary C Mahony ◽  
David F Archer
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Vascular Endothelial ◽  
Ishikawa Cells ◽  
Endothelial Growth Factor ◽  
Vegf Isoforms

Addition of a c-myc epitope tag within the VEGF protein does not affect in vitro biological activity

2001 ◽  
Vol 79 (1) ◽  
pp. 107-112 ◽  
Author(s):  
Olivier Chavand ◽  
Katrina Spilsbury ◽  
Piroska E Rakoczy
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Retinal Pigment ◽  
Retinal Pigment Epithelial Cells ◽  
Endothelial Cell Proliferation ◽  
Vascular Endothelial ◽  
Heparin Binding ◽  
Epitope Tag ◽  
Endothelial Growth Factor ◽  
Vegf Isoforms

The overexpression of vascular endothelial growth factor (VEGF) has been strongly implicated in diseases involving neovascularization. VEGF exists in as many as six different isoforms, each showing a unique pattern of tissue distribution and activity. To investigate the effect of individual VEGF isoform overexpression in neovascular disease models, we inserted c-myc epitope tags into the three VEGF isoforms expressed in retinal pigment epithelial cells, VEGF121, VEGF165, and VEGF189. We found that the 12-amino acid insertion between the receptor binding and heparin binding domains did not affect VEGF transcription, translation, or secretion. In addition, VEGF isoforms containing the c-myc epitope tag were able to stimulate endothelial cell proliferation as efficiently as non-tagged VEGF isoforms and they could be individually identified by Western blotting and immunocytochemistry using the c-myc epitope specific monoclonal antibody 9E10.Key words: Vascular endothelial growth factor, VEGF, c-myc epitope tag, immunocytochemistry.

Effects of Vascular Endothelial Growth Factor (VEGF) Isoforms on Rat Testis Composition and Germ Cell Numbers.

2009 ◽  
Vol 81 (Suppl_1) ◽  
pp. 20-20
Author(s):  
Ningxia Lu ◽  
Shantille Kruse ◽  
Racheal Slattery ◽  
Debra Clopton ◽  
Andrea S. Cupp
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Germ Cell ◽  
Vascular Endothelial ◽  
Cell Numbers ◽  
Rat Testis ◽  
Endothelial Growth Factor ◽  
Vegf Isoforms
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