Effects of Vascular Endothelial Growth Factor (VEGF) Isoforms on Rat Testis Composition and Germ Cell Numbers.

2009 ◽  
Vol 81 (Suppl_1) ◽  
pp. 20-20
Author(s):  
Ningxia Lu ◽  
Shantille Kruse ◽  
Racheal Slattery ◽  
Debra Clopton ◽  
Andrea S. Cupp
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Germ Cell ◽  
Vascular Endothelial ◽  
Cell Numbers ◽  
Rat Testis ◽  
Endothelial Growth Factor ◽  
Vegf Isoforms

scholarly journals Expression of Vascular Endothelial Growth Factor (VEGF) Isoforms VEGFxxxb in Normal Human Renal Development

2006 ◽  
Vol 20 (4) ◽  
Author(s):  
Heather Samantha Bevan ◽  
Nynke N.M.S van den Akker ◽  
Japke A.E Polman ◽  
Steve J Harper ◽  
Adriana C Gittenberger‐de Groot ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Renal Development ◽  
Vascular Endothelial ◽  
Normal Human ◽  
Endothelial Growth Factor ◽  
Vegf Isoforms

scholarly journals Expression of vascular endothelial growth factor in patients with testicular germ cell tumors as an indicator of metastatic disease

Cancer ◽  
1999 ◽  
Vol 85 (6) ◽  
pp. 1323-1330 ◽  
Author(s):  
Souichirou Fukuda ◽  
Tsutomu Shirahama ◽  
Yoshiharu Imazono ◽  
Tomoyasu Tsushima ◽  
Hiroyuki Ohmori ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Germ Cell ◽  
Metastatic Disease ◽  
Germ Cell Tumors ◽  
Vascular Endothelial ◽  
Testicular Germ Cell Tumors ◽  
Testicular Germ Cell ◽  
Endothelial Growth Factor

scholarly journals The vascular endothelial growth factor (VEGF) isoforms: differential deposition into the subepithelial extracellular matrix and bioactivity of extracellular matrix-bound VEGF.

1993 ◽  
Vol 4 (12) ◽  
pp. 1317-1326 ◽  
Author(s):  
J E Park ◽  
G A Keller ◽  
N Ferrara
Keyword(s):  
Extracellular Matrix ◽  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Vascular Endothelial ◽  
Vegf Mrna ◽  
Angiogenic Potential ◽  
Molecular Masses ◽  
Matrix Bound ◽  
Endothelial Growth Factor ◽  
Vegf Isoforms

Vascular endothelial growth factor (VEGF)mRNA undergoes alternative splicing events that generate four different homodimeric isoforms, VEGF121, VEGF165, VEGF189, or VEGF206. VEGF121 is a nonheparin-binding acidic protein, which is freely diffusible. The longer forms, VEGF189 or VEGF206, are highly basic proteins tightly bound to extracellular heparin-containing proteoglycans. VEGF165 has intermediate properties. To determine the localization of VEGF isoforms, transfected human embryonic kidney CEN4 cells expressing VEGF165, VEGF189, or VEGF206 were stained by immunofluorescence with a specific monoclonal antibody. The staining was found in patches and streaks suggestive of extracellular matrix (ECM). VEGF165 was observed largely in Golgi apparatus-like structures. Immunogold labeling of cells expressing VEGF189 or VEGF206 revealed that the staining was localized to the subepithelial ECM. VEGF associated with the ECM was bioactive, because endothelial cells cultured on ECM derived from cells expressing VEGF189 or VEGF206 were markedly stimulated to proliferate. In addition, ECM-bound VEGF can be released into a soluble and bioactive form by heparin or plasmin. ECM-bound VEGF189 and VEGF206 have molecular masses consistent with the intact polypeptides. The ECM may represent an important source of VEGF and angiogenic potential.

scholarly journals Vascular endothelial growth factor regulates germ cell survival during establishment of spermatogenesis in the bovine testis

Reproduction ◽  
2009 ◽  
Vol 138 (4) ◽  
pp. 667-677 ◽  
Author(s):  
Kyle C Caires ◽  
Jeanene de Avila ◽  
Derek J McLean
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Germ Cell ◽  
Cell Survival ◽  
Germ Cells ◽  
Pcr Analysis ◽  
Vascular Endothelial ◽  
Endothelial Growth Factor ◽  
Testis Tissue

Vascular endothelial growth factor-A (VEGFA) is a hypoxia-inducible peptide essential for angiogenesis and targets nonvascular cells in a variety of tissues and cell types. The objective of the current study was to determine the function of VEGF during testis development in bulls. We used an explant tissue culture and treatment approach to test the hypothesis that VEGFA-164 could regulate the biological activity of bovine germ cells. We demonstrate that VEGFA, KDR, and FLT1 proteins are expressed in germ and somatic cells in the bovine testis. Treatment of bovine testis tissue with VEGFA in vitro resulted in significantly more germ cells following 5 days of culture when compared with controls. Quantitative real-time RT-PCR analysis determined that VEGF treatment stimulated an intracellular response that prevents germ cell death in bovine testis tissue explants, as indicated by increased expression of BCL2 relative to BAX and decreased expression of BNIP3 at 3, 6, and 24 h during culture. Blocking VEGF activity in vitro using antisera against KDR and VEGF significantly reduced the number of germ cells in VEGF-treated testis tissue to control levels at 120 h. Testis grafting provided in vivo evidence that bovine testis tissue treated with VEGFA for 5 days in culture contained significantly more differentiating germ cells compared with controls. These findings support the conclusion that VEGF supports germ cell survival and sperm production in bulls.

Cell migration and growth induced by photo-immobilised vascular endothelial growth factor (VEGF) isoforms

2019 ◽  
Vol 7 (27) ◽  
pp. 4272-4279 ◽  
Author(s):  
Xueli Ren ◽  
Jun Akimoto ◽  
Hideyuki Miyatake ◽  
Seiichi Tada ◽  
Liping Zhu ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Cell Migration ◽  
Growth Factor ◽  
Vascular Endothelial ◽  
Cell Migration And Proliferation ◽  
Endothelial Growth Factor ◽  
Vegf Isoforms

VEGF isoforms immobilised by photo-reactive gelatin (AzPhe-gelatin) enhance cell migration and proliferation.

Effect of Tibolone and its metabolites on vascular endothelial growth factor (VEGF) isoforms 121 and 165 in ishikawa cells

2002 ◽  
Vol 78 ◽  
pp. S159-S160
Author(s):  
Sebastian Mirkin ◽  
Susan Leslie ◽  
Michelle Billeter ◽  
Mary C Mahony ◽  
David F Archer
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Vascular Endothelial ◽  
Ishikawa Cells ◽  
Endothelial Growth Factor ◽  
Vegf Isoforms

Addition of a c-myc epitope tag within the VEGF protein does not affect in vitro biological activity

2001 ◽  
Vol 79 (1) ◽  
pp. 107-112 ◽  
Author(s):  
Olivier Chavand ◽  
Katrina Spilsbury ◽  
Piroska E Rakoczy
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Retinal Pigment ◽  
Retinal Pigment Epithelial Cells ◽  
Endothelial Cell Proliferation ◽  
Vascular Endothelial ◽  
Heparin Binding ◽  
Epitope Tag ◽  
Endothelial Growth Factor ◽  
Vegf Isoforms

The overexpression of vascular endothelial growth factor (VEGF) has been strongly implicated in diseases involving neovascularization. VEGF exists in as many as six different isoforms, each showing a unique pattern of tissue distribution and activity. To investigate the effect of individual VEGF isoform overexpression in neovascular disease models, we inserted c-myc epitope tags into the three VEGF isoforms expressed in retinal pigment epithelial cells, VEGF121, VEGF165, and VEGF189. We found that the 12-amino acid insertion between the receptor binding and heparin binding domains did not affect VEGF transcription, translation, or secretion. In addition, VEGF isoforms containing the c-myc epitope tag were able to stimulate endothelial cell proliferation as efficiently as non-tagged VEGF isoforms and they could be individually identified by Western blotting and immunocytochemistry using the c-myc epitope specific monoclonal antibody 9E10.Key words: Vascular endothelial growth factor, VEGF, c-myc epitope tag, immunocytochemistry.

Sign in / Sign up

Export Citation Format

Share Document