Changes in Several Inflammatory and Oxidation Markers after Ovalbumin-Sensitization in a Guinea Pig Model of Allergic Asthma - A Pilot Study
Ovalbumin (OVA)-sensitization is a common way to evoke changes similar to changes in allergic asthma in humans. Activated cells produce various pro-inflammatory and vasoactive substances including reactive oxygen species. The goal of this pilot study was to evaluate mobilization of leukocytes into the lungs and oxidation processes induced by OVA-sensitization in a guinea-pig model of allergic asthma. Guinea-pigs were divided into OVA-sensitized and naïve animals. After sacrificing animals, blood samples were taken and total and differential leukocyte counts were calculated, and eosinophil cationic protein (ECP) and total antioxidant status (TAS) in the plasma were determined. Left lungs were saline-lavaged and total number of cells and differential leukocyte count in the bronchoalveolar lavage fluid (BAL) were calculated. Right lung tissue was homogenized, ECP, TAS and products of lipid and protein oxidation (thiobarbituric acid-reactive substances and lysine-lipoperoxidation end-products) were determined in the lung homogenate. OVA-sensitization increased a total number of cells and percentages of eosinophils and neutrophils and slightly increased ECP in the blood and in the BAL fluid. In addition, increased lipid and protein oxidation in the lung homogenate, and decreased TAS in the plasma was found in OVA-sensitized compared to naïve animals. In conclusion, OVA-sensitization increased mobilization of leukocytes into the lungs and elevated production of reactive oxygen spesies (ROS), accompanied by a decrease in plasma TAS.
Measurement and Inhibition of Airway Hyperreactivity in a Guinea Pig Model of Allergic Asthma
