Montelukast Sodium Formulation Containing Green Tea Extract to Reduce the Oxidative Stress in Guinea Pig Model of Chronic Allergic Asthma

Ischemic stroke results in brain damage and behavioral deficits including memory impairment. Protective effects of green tea extract (GTex) and its major functional polyphenol (−)-epigallocatechin gallate (EGCG) on memory were examined in cerebral ischemic rats. GTex and EGCG were administered 1 hr before middle cerebral artery ligation in rats. GTex, EGCG, and pentoxifylline (PTX) significantly improved ishemic-induced memory impairment in a Morris water maze test. Malondialdehyde (MDA) levels, glutathione (GSH), and superoxide dismutase (SOD) activity in the cerebral cortex and hippocampus were increased by long-term treatment with GTex and EGCG. Both compounds were also associated with reduced cerebral infraction breakdown of MDA and GSH in the hippocampus. Inin vitroexperiments, EGCG had anti-inflammatory effects in BV-2 microglia cells. EGCG inhibited lipopolysaccharide- (LPS-) induced nitric oxide production and reduced cyclooxygenase-2 and inducible nitric oxide synthase expression in BV-2 cells. GTex and its active polyphenol EGCG improved learning and memory deficits in a cerebral ischemia animal model and such protection may be due to the reduction of oxidative stress and neuroinflammation.

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Effect of Green Tea Extract-Enriched Diets on Insulin and Leptin Levels, Oxidative Stress Parameters and Antioxidant Enzymes Activities in Obese Mice

Polish Journal of Food and Nutrition Sciences ◽

10.1515/pjfns-2017-0004 ◽

2017 ◽

Vol 67 (3) ◽

pp. 233-240 ◽

Cited By ~ 1

Author(s):

Hana Bártíková ◽

Iva Boušová ◽

Petra Matoušková ◽

Barbora Szotáková ◽

Lenka Skálová

Keyword(s):

Oxidative Stress ◽

Antioxidant Enzymes ◽

Green Tea ◽

Green Tea Extract ◽

Obese Mice ◽

Enzymes Activities ◽

Oxidative Stress Parameters ◽

Antioxidant Enzymes Activities ◽

Tea Extract ◽

Stress Parameters

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Green Tea Potentially Ameliorates Bisphenol A-Induced Oxidative Stress: AnIn VitroandIn SilicoStudy

Biochemistry Research International ◽

10.1155/2014/259763 ◽

2014 ◽

Vol 2014 ◽

pp. 1-9 ◽

Cited By ~ 14

Author(s):

Hiral Suthar ◽

R. J. Verma ◽

Saumya Patel ◽

Y. T. Jasrai

Keyword(s):

Oxidative Stress ◽

Bisphenol A ◽

Green Tea ◽

Binding Capacity ◽

Venous Blood ◽

Green Tea Extract ◽

High Dose ◽

Healthy Human ◽

Tea Extract ◽

Dose Dependent

The present investigation was an attempt to elucidate oxidative stress induced by bisphenol A on erythrocytes and its amelioration by green tea extract. For this, venous blood samples from healthy human adults were collected in EDTA vials and used for preparation of erythrocytes suspension. When erythrocyte suspensions were treated with different concentrations of BPA/H2O2, a dose-dependent increase in hemolysis occurred. Similarly, when erythrocytes suspensions were treated with either different concentrations ofH2O2(0.05–0.25 mM) along with BPA (50 μg/mL) or 0.05 mMH2O2along with different concentrations of BPA (50–250 μg/mL), dose-dependent significant increase in hemolysis occurred. The effect of BPA andH2O2was found to be additive. For the confirmation, binding capacity of bisphenol A with erythrocyte proteins (hemoglobin, catalase, and glutathione peroxidase) was inspected using molecular docking tool, which showed presence of various hydrogen bonds of BPA with the proteins. The present data clearly indicates that BPA causes oxidative stress in a similar way asH2O2. Concurrent addition of different concentrations (10–50 μg/mL) of green tea extract to reaction mixture containing high dose of bisphenol A (250 μg/mL) caused concentration-dependent amelioration in bisphenol A-induced hemolysis. The effect was significant (P<0.05). It is concluded that BPA-induced oxidative stress could be significantly mitigated by green tea extract.

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Effect of Green Tea Extract on Exercise-Induced Oxidative Stress in Obese Men: A Randomized, Double-Blind, Placebo-Controlled, Crossover Study

Asian Journal of Sports Medicine ◽

10.5812/asjsm.55438 ◽

2017 ◽

Vol In Press (In Press) ◽

Cited By ~ 1

Author(s):

Rahman Rahimi ◽

Zahed Falahi

Keyword(s):

Oxidative Stress ◽

Crossover Study ◽

Green Tea ◽

Green Tea Extract ◽

Double Blind ◽

Double Blind Placebo ◽

Exercise Induced ◽

Tea Extract

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Changes in Several Inflammatory and Oxidation Markers after Ovalbumin-Sensitization in a Guinea Pig Model of Allergic Asthma - A Pilot Study

Acta Medica Martiniana ◽

10.2478/v10201-011-0032-6 ◽

2012 ◽

Vol 12 (2) ◽

pp. 5-11 ◽

Cited By ~ 1

Author(s):

D Mokra ◽

A Drgova ◽

R Pullmann ◽

P Mikolka ◽

M Antosova ◽

...

Keyword(s):

Pilot Study ◽

Guinea Pig ◽

Allergic Asthma ◽

Protein Oxidation ◽

Reactive Oxygen ◽

Lung Homogenate ◽

Pig Model ◽

Lipid And Protein Oxidation ◽

Guinea Pig Model ◽

Number Of Cells

Changes in Several Inflammatory and Oxidation Markers after Ovalbumin-Sensitization in a Guinea Pig Model of Allergic Asthma - A Pilot Study Ovalbumin (OVA)-sensitization is a common way to evoke changes similar to changes in allergic asthma in humans. Activated cells produce various pro-inflammatory and vasoactive substances including reactive oxygen species. The goal of this pilot study was to evaluate mobilization of leukocytes into the lungs and oxidation processes induced by OVA-sensitization in a guinea-pig model of allergic asthma. Guinea-pigs were divided into OVA-sensitized and naïve animals. After sacrificing animals, blood samples were taken and total and differential leukocyte counts were calculated, and eosinophil cationic protein (ECP) and total antioxidant status (TAS) in the plasma were determined. Left lungs were saline-lavaged and total number of cells and differential leukocyte count in the bronchoalveolar lavage fluid (BAL) were calculated. Right lung tissue was homogenized, ECP, TAS and products of lipid and protein oxidation (thiobarbituric acid-reactive substances and lysine-lipoperoxidation end-products) were determined in the lung homogenate. OVA-sensitization increased a total number of cells and percentages of eosinophils and neutrophils and slightly increased ECP in the blood and in the BAL fluid. In addition, increased lipid and protein oxidation in the lung homogenate, and decreased TAS in the plasma was found in OVA-sensitized compared to naïve animals. In conclusion, OVA-sensitization increased mobilization of leukocytes into the lungs and elevated production of reactive oxygen spesies (ROS), accompanied by a decrease in plasma TAS.

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