Aerosolized Polymerized Type I Collagen Reduces Airway Inflammation and Remodelling in a Guinea Pig Model of Allergic Asthma

Background. To investigate Wnt/β-catenin signaling pathway expression and its regulation of type I collagen by TGF-β1 in scleral fibroblasts from form-deprivation myopia (FDM) guinea pig model.Methods. Wnt isoforms were examined using genome microarrays. Scleral fibroblasts from FDM group and self-control (SC) group were cultured. Wnt isoforms,β-catenin, TGF-β1, and type I collagen expression levels were examined in the two groups with or without DKK-1 or TGF-β1 neutralizing antibody.Results. For genome microarrays, the expression of Wnt3 in FDM group was significantly greater as confirmed in retinal and scleral tissue. The expression of Wnt3 andβ-catenin significantly increased in FDM group and decreased significantly with DKK-1. TGF-β1 expression level decreased significantly in FDM group and increased significantly with DKK-1. Along with morphological misalignment inside and outside cells, the amount of type I collagen decreased in FDM group. Furthermore, type I collagen increased and became regular in DKK-1 intervention group, whereas it decreased and rearranged more disorder in TGF-β1 neutralizing antibody intervention group.Conclusions. The activation of Wnt3/β-catenin signaling pathway was demonstrated in primary scleral fibroblasts in FDM. This pathway further reduced the expression of type I collagen by TGF-β1, which ultimately played a role in scleral remodeling during myopia development.

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Montelukast Sodium Formulation Containing Green Tea Extract to Reduce the Oxidative Stress in Guinea Pig Model of Chronic Allergic Asthma

Recent Patents on Drug Delivery & Formulation ◽

10.2174/1872211313666181211123903 ◽

2019 ◽

Vol 12 (4) ◽

pp. 267-276

Author(s):

Neelam Singh ◽

Giriraj T. Kulkarni ◽

Yatendra Kumar

Keyword(s):

Oxidative Stress ◽

Guinea Pig ◽

Allergic Asthma ◽

Green Tea ◽

Green Tea Extract ◽

Pig Model ◽

Montelukast Sodium ◽

Tea Extract ◽

Guinea Pig Model

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Ceramide: a Key Signaling Molecule in a Guinea Pig Model of Allergic Asthmatic Response and Airway Inflammation

Journal of Pharmacology and Experimental Therapeutics ◽

10.1124/jpet.107.131565 ◽

2007 ◽

Vol 324 (2) ◽

pp. 548-557 ◽

Cited By ~ 48

Author(s):

Emanuela Masini ◽

Lucia Giannini ◽

Silvia Nistri ◽

Lorenzo Cinci ◽

Rosanna Mastroianni ◽

...

Keyword(s):

Guinea Pig ◽

Airway Inflammation ◽

Pig Model ◽

Signaling Molecule ◽

Asthmatic Response ◽

Allergic Asthmatic ◽

Guinea Pig Model

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Changes in Several Inflammatory and Oxidation Markers after Ovalbumin-Sensitization in a Guinea Pig Model of Allergic Asthma - A Pilot Study

Acta Medica Martiniana ◽

10.2478/v10201-011-0032-6 ◽

2012 ◽

Vol 12 (2) ◽

pp. 5-11 ◽

Cited By ~ 1

Author(s):

D Mokra ◽

A Drgova ◽

R Pullmann ◽

P Mikolka ◽

M Antosova ◽

...

Keyword(s):

Pilot Study ◽

Guinea Pig ◽

Allergic Asthma ◽

Protein Oxidation ◽

Reactive Oxygen ◽

Lung Homogenate ◽

Pig Model ◽

Lipid And Protein Oxidation ◽

Guinea Pig Model ◽

Number Of Cells

Changes in Several Inflammatory and Oxidation Markers after Ovalbumin-Sensitization in a Guinea Pig Model of Allergic Asthma - A Pilot Study Ovalbumin (OVA)-sensitization is a common way to evoke changes similar to changes in allergic asthma in humans. Activated cells produce various pro-inflammatory and vasoactive substances including reactive oxygen species. The goal of this pilot study was to evaluate mobilization of leukocytes into the lungs and oxidation processes induced by OVA-sensitization in a guinea-pig model of allergic asthma. Guinea-pigs were divided into OVA-sensitized and naïve animals. After sacrificing animals, blood samples were taken and total and differential leukocyte counts were calculated, and eosinophil cationic protein (ECP) and total antioxidant status (TAS) in the plasma were determined. Left lungs were saline-lavaged and total number of cells and differential leukocyte count in the bronchoalveolar lavage fluid (BAL) were calculated. Right lung tissue was homogenized, ECP, TAS and products of lipid and protein oxidation (thiobarbituric acid-reactive substances and lysine-lipoperoxidation end-products) were determined in the lung homogenate. OVA-sensitization increased a total number of cells and percentages of eosinophils and neutrophils and slightly increased ECP in the blood and in the BAL fluid. In addition, increased lipid and protein oxidation in the lung homogenate, and decreased TAS in the plasma was found in OVA-sensitized compared to naïve animals. In conclusion, OVA-sensitization increased mobilization of leukocytes into the lungs and elevated production of reactive oxygen spesies (ROS), accompanied by a decrease in plasma TAS.

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