scholarly journals Molecular mechanisms of the crosstalk between mitochondrial and NADPH oxidase derived reactive oxygen species in white blood cells – implications for cardiovascular diseases

2012 ◽  
Vol 26 (S1) ◽  
Author(s):  
Sebastian Steven ◽  
Swenja Schuhmacher ◽  
Philip Wenzel ◽  
Matthias Oelze ◽  
Steffen Daub ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Cardiovascular Diseases ◽  
Nadph Oxidase ◽  
Blood Cells ◽  
Molecular Mechanisms ◽  
White Blood Cells ◽  
Reactive Oxygen ◽  
Oxygen Species

scholarly journals Molecular Mechanisms of the Crosstalk Between Mitochondria and NADPH Oxidase Through Reactive Oxygen Species—Studies in White Blood Cells and in Animal Models

2014 ◽  
Vol 20 (2) ◽  
pp. 247-266 ◽  
Author(s):  
Swenja Kröller-Schön ◽  
Sebastian Steven ◽  
Sabine Kossmann ◽  
Alexander Scholz ◽  
Steffen Daub ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Animal Models ◽  
Nadph Oxidase ◽  
Blood Cells ◽  
Molecular Mechanisms ◽  
White Blood Cells ◽  
Reactive Oxygen ◽  
Oxygen Species

scholarly journals β-Aminobutyric Acid Primes an NADPH Oxidase–Dependent Reactive Oxygen Species Production During Grapevine-Triggered Immunity

2010 ◽  
Vol 23 (8) ◽  
pp. 1012-1021 ◽  
Author(s):  
Carole Dubreuil-Maurizi ◽  
Sophie Trouvelot ◽  
Patrick Frettinger ◽  
Alain Pugin ◽  
David Wendehenne ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Nadph Oxidase ◽  
Molecular Mechanisms ◽  
Reactive Oxygen ◽  
Aminobutyric Acid ◽  
Ros Production ◽  
Oxygen Species ◽  
Defense Gene Expression ◽  
Diphenylene Iodonium ◽  
Signaling Events

The molecular mechanisms underlying the process of priming are poorly understood. In the present study, we investigated the early signaling events triggered by β-aminobutyric acid (BABA), a well-known priming-mediated plant resistance inducer. Our results indicate that, in contrast to oligogalacturonides (OG), BABA does not elicit typical defense-related early signaling events nor defense-gene expression in grapevine. However, in OG-elicited cells pretreated with BABA, production of reactive oxygen species (ROS) and expression of the respiratory-burst oxidase homolog RbohD gene were primed. In response to the causal agent of downy mildew Plasmopara viticola, a stronger ROS production was specifically observed in BABA-treated leaves. This process was correlated with an increased resistance. The NADPH oxidase inhibitor diphenylene iodonium (DPI) abolished this primed ROS production and reduced the BABA-induced resistance (BABA-IR). These results suggest that priming of an NADPH oxidase–dependent ROS production contributes to BABA-IR in the Vitis-Plasmopara pathosystem.

scholarly journals A new hydrophilic polysulfone hemodialysis membrane can prevent platelet–neutrophil interactions and successive neutrophil activation

2019 ◽  
Vol 42 (4) ◽  
pp. 175-181 ◽  
Author(s):  
Yoko Koga ◽  
Hiroyuki Meguro ◽  
Hiroaki Fujieda ◽  
Yoshiyuki Ueno ◽  
Keishi Miwa ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Blood Cells ◽  
Cell Activation ◽  
Molecular Mechanisms ◽  
Reactive Oxygen ◽  
Neutrophil Activation ◽  
Human Neutrophils ◽  
Oxygen Species ◽  
Polysulfone Membrane ◽  
Polysulfone Membranes

Purpose: Microaggregates have often been observed during hemodialysis and are clearly associated with complications of hemodialysis therapy. In this study, we aimed to clarify the effects of two polysulfone membranes, with different abilities to activate blood cells, on the formation of these microaggregates; we also investigated their molecular mechanisms. Methods: Human whole blood was circulated through a mini-module dialyzer using the membranes in vitro; platelet–neutrophil complexes in blood were determined by flow cytometry. Isolated human neutrophils were incubated with the membranes in plasma, in the presence or absence of platelets, followed by flow cytometric analysis of intracellular reactive oxygen species and cell-surface activated CD11b on neutrophils. Results: CX-U, a conventional polysulfone membrane with remarkable cell activation, induced the formation of platelet–neutrophil complexes; however, NV-U, a new hydrophilic polysulfone membrane with slight or no cell activation, did not cause complex formation. Moreover, CX-U-induced reactive oxygen species production and the increase in activated CD11b expression on neutrophils were enhanced by platelets. On the other hand, NV-U hardly affected neutrophil activation, regardless of whether platelets were present or not. The enhancement of CX-U-induced neutrophil activations by platelets was greatly inhibited by anti-CD62P antibody. Conclusion: The ability of polysulfone membranes to activate blood cells is closely related to platelet–neutrophil interaction. Therefore, a biocompatible membrane, like NV-U, can be expected to prevent microaggregate formation during hemodialysis and avoid subsequent cell activation.

scholarly journals Reactive Oxygen Species Production and Mitochondrial Dysfunction in White Blood Cells Are Not Valid Biomarkers of Ageing in the Very Old

PLoS ONE ◽  
2014 ◽  
Vol 9 (3) ◽  
pp. e91005 ◽  
Author(s):  
Laura Wiley ◽  
Deepthi Ashok ◽  
Carmen Martin-Ruiz ◽  
Duncan C. S. Talbot ◽  
Joanna Collerton ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Mitochondrial Dysfunction ◽  
Blood Cells ◽  
Reactive Oxygen Species Production ◽  
White Blood Cells ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Very Old ◽  
Species Production
Sign in / Sign up

Export Citation Format

Share Document