TNF‐α/IL‐1β—licensed mesenchymal stromal cells promote corneal allograft survival
            via
            myeloid cell‐mediated induction of Foxp3
            +
            regulatory T cells in the lung

Inflammatory response mediated by immune cells is either directly or indirectly regulated by mesenchymal stromal cells (MSCs). Accumulating evidence suggests that thrombospondin-1 (TSP-1) is highly expressed in response to inflammation. In this work, we isolated and identified human thymic mesenchymal stromal cells (tMSCs) and detected the expression of TSP-1. We found that tMSCs expressed TSP-1 and Poly (I: C) or LPS treatment promoted the expression of TSP-1. Further, we isolated and identified exosomes originating from tMSCs (MEXs). Notably, exosomes derived from LPS-pretreated tMSCs (MEXsLPS) promoted the polarization of macrophages to M1-like phenotype and IL-6, TNF-α secretion as well as the pro-inflammatory differentiation of CD4+T cells into Th17 cells. Upon silencing the expression of TSP-1 in tMSCs, the pro-inflammatory effects of MEXsLPS were suppressed. Therefore, these findings uncovered TSP-1 as the principal factor in MEXsLPS pro-inflammatory regulation.

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Mesenchymal stromal cells and regulatory T cells: the Yin and Yang of peripheral tolerance?

Immunology and Cell Biology ◽

10.1038/icb.2012.60 ◽

2012 ◽

Vol 91 (1) ◽

pp. 12-18 ◽

Cited By ~ 87

Author(s):

Stephen P Burr ◽

Francesco Dazzi ◽

Oliver A Garden

Keyword(s):

T Cells ◽

Regulatory T Cells ◽

Mesenchymal Stromal Cells ◽

Stromal Cells ◽

Peripheral Tolerance ◽

Yin And Yang

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P.78 BONE MARROW-DERIVED MESENCHYMAL STROMAL CELLS LOCALLY INJECTED INDUCE AN UP-REGULATION OF BOTH CIRCULATING AND MUCOSAL FOXP3+ REGULATORY T-CELLS IN PATIENTS WITH PERIANAL CROHN'S DISEASE

Digestive and Liver Disease ◽

10.1016/s1590-8658(10)60191-0 ◽

2010 ◽

Vol 42 ◽

pp. S130-S131

Author(s):

R. Ciccocioppo ◽

M. Bernardo ◽

M. Russo ◽

A. Avanzini ◽

A. Sgarella ◽

...

Keyword(s):

Bone Marrow ◽

T Cells ◽

Crohn’S Disease ◽

Crohn's Disease ◽

Regulatory T Cells ◽

Mesenchymal Stromal Cells ◽

Stromal Cells ◽

Perianal Crohn’S Disease ◽

Perianal Crohn's Disease

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The Balance of Th1/Th2 and LAP+Tregs/Th17 Cells Is Crucial for Graft Survival in Allogeneic Corneal Transplantation

Journal of Ophthalmology ◽

10.1155/2018/5404989 ◽

2018 ◽

Vol 2018 ◽

pp. 1-10 ◽

Cited By ~ 4

Author(s):

Shang Li ◽

Jing Yu ◽

Chungang Guo ◽

Ying Jie ◽

Zhiqiang Pan

Keyword(s):

T Cells ◽

Regulatory T Cells ◽

Aqueous Humor ◽

Allograft Rejection ◽

Corneal Transplantation ◽

Allograft Survival ◽

Survival Group ◽

Corneal Allograft ◽

Group 3 ◽

Corneal Allograft Rejection

Purpose. CD4+LAP+ T cells are newly discovered regulatory T cells (Tregs). The aim of this study is to investigate the balance of Th1/Th2 and LAP+Tregs/Th17 in mice after allogeneic corneal transplantation. Methods. A total of 65 mice received orthotopic penetrating transplantation. According to the survival scores of the grafts, the mice were divided into the rejection group and the survival group 3 weeks after transplantation. Th1, Th2, Th17, and regulatory T cells in the ipsilateral drainage lymph nodes and spleens were measured with flow cytometry. The related cytokines in aqueous humor were also analyzed. Results. The frequencies of Foxp3+Tregs, GARP+Tregs, and LAP+Tregs in the survival group were significantly higher than those in the rejection group. And the expression trend of CD4+LAP+ T cells and CD4+GARP+ T cells was consistent. The level of IFN-γ, TNF, IL-6, and IL-17A markedly increased in aqueous humor during corneal allograft rejection. The ratio of Th1/Th2 and Th17/LAP+Tregs significantly increased in the rejection group at the 3rd week after corneal transplantation. Conclusion. LAP+Tregs might be regarded as substitute for Foxp3+Tregs. The balance of Th1/Th2 and LAP+Tregs/Th17 is crucial for corneal allograft survival.

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