Deficiency of core fucosylation activates cellular signaling dependent on FLT3 expression in a Ba/F3 cell system

Growth of cell system (BeWo-cell line) derived from human gestational choriocarcinoma has been established and continuously maintained in-vitro. Furthermore, it is evident from the previous studies that this cell line has retained the physiological function of the placental trophoblasts, namely the synthesis of human chorionic gonadotrophil(HCG).The BeWo cells were relatively small and possessed single nuclei, thus indicating that this cell line consists exclusively of cytotrophoblasts. In some instances cells appeared widely separated and their lateral surfaces were provided with numerous microvilli (Fig.1).

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The novel histamine H2 receptor antagonist FRG-8813 prevents delay of wound repair induced by hydrogen peroxide in a rabbit gastric epithelial cell system

Journal of Gastroenterology and Hepatology ◽

10.1046/j.1440-1746.1998.01735.x ◽

1998 ◽

Vol 13 (11-s4) ◽

pp. S209-S213

Author(s):

NOBUHIRO SATO ◽

SUMIO WATANABE ◽

XIAN-EN WANG ◽

TARO OSADA ◽

HIROSHI TANAKA ◽

...

Keyword(s):

Hydrogen Peroxide ◽

Epithelial Cell ◽

Receptor Antagonist ◽

Wound Repair ◽

Cell System ◽

The Novel ◽

H2 Receptor Antagonist ◽

Histamine H2 Receptor ◽

H2 Receptor ◽

Histamine H2 Receptor Antagonist

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Electrical automobile having a fuel cell, and method of powering an electrical automobile with a fuel cell system

Journal of Power Sources ◽

10.1016/s0378-7753(97)83990-0 ◽

1998 ◽

Vol 70 (1) ◽

pp. 131

Author(s):

J Werth

Keyword(s):

Fuel Cell ◽

Cell System ◽

Fuel Cell System

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High Level Expression of Active Human Prothrombin in a Vaccina Virus Expression System

Thrombosis and Haemostasis ◽

10.1055/s-0038-1656335 ◽

1992 ◽

Vol 68 (02) ◽

pp. 119-124 ◽

Cited By ~ 10

Author(s):

F G Falkner ◽

P L Turecek ◽

R T A MacGillivray ◽

W Bodemer ◽

F Scheiflinger ◽

...

Keyword(s):

Vaccinia Virus ◽

Cell Lines ◽

Expression System ◽

Human Cell Line ◽

Cell System ◽

Time Saving ◽

Expression Levels ◽

Mammalian Cell Lines ◽

Cell Line Hela ◽

Virus Expression

SummaryWe have worked out an efficient and time saving procedure for the expression of recombinant human prothrombin. The glycoprotein was expressed in the vaccinia virus expression system in several mammalian cell lines. The kidney cell lines Vero and BHK and the human cell line Hela were found to efficiently secrete prothrombin. Expression levels of 3–4 µg of factor II per 106 cells per day corresponding to 18–23 mU per 106 cells per day were achieved. Since the expression levels obtained with the vaccinia virus/Vero cell system were comparable to those obtained in amplified transformed CHO cells it provides an alternative system for the efficient expression of human prothrombin and may allow to further elucidate structure-function relationships of (pro)thrombin and its various effectors.

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