scholarly journals THE EFFICACY OF HEMOGLOBIN-VESICLE, AN ARTIFICIAL OXYGEN CARRIER, IN HEMORRHAGIC SHOCK RESUSCITATION, AND CARDIOPULMONARY BYPASS

ASAIO Journal ◽  
2005 ◽  
Vol 51 (2) ◽  
pp. 48A
Author(s):  
Koichi Kobayashi ◽  
Hiromi Sakai ◽  
Masataka Yamazaki ◽  
Manabu Yamamoto ◽  
Hirohisa Horinouchi ◽  
...  
Keyword(s):  
Cardiopulmonary Bypass ◽  
Hemorrhagic Shock ◽  
Oxygen Carrier ◽  
Artificial Oxygen Carrier

Abstract 4030: Resuscitation with Intraosseous Infusion of Artificial Oxygen Carrier of Liposome-Encapsulated Hemoglobin (LHb) from Lethal Hemorrhagic Shock and Its Effect on Cytokines

Circulation ◽  
2008 ◽  
Vol 118 (suppl_18) ◽  
Author(s):  
Bonpei Takase ◽  
Satoshi Shono ◽  
Manabu Kinoshita ◽  
Yashiro Nogami ◽  
Yoshitaka Ogata ◽  
...  
Keyword(s):  
Renal Function ◽  
Side Effects ◽  
Hemorrhagic Shock ◽  
Oxygen Carrier ◽  
Femoral Vein ◽  
Survival Rates ◽  
Blood Substitute ◽  
Disaster Medicine ◽  
Mice Model ◽  
Artificial Oxygen Carrier

Liposome-encapsulated hemoglobin (LHb), which is structurally similar to red blood cells (RBC) except smaller size (250 nm), can serve as blood substitute comparable to RBC. We have reported that intraosseous blood infusion (IOI) is effective treatment in shock mice model. IOI is alternative to peripheral i.v. infusion and is expected as an important field treatment in civilian emergency because of no collapse of intramedullary blood vessels in the bone marrow in shock. However, we did not evaluate the side effects of LHb in IOI. Total 70% hemorrhagic shock was induced by femoral vein bleeding. Immediately after bleeding, 17 mice were resuscitated with tibial bone IOI of 5% albumin (5% albumin), 18 mice resuscitated with mouse-washed RBC (Wash RBC) and 14 mice resuscitated with LHb (LHb-group). Survival rates were compared and the temporal changes in cytokins (TNF, INFγ) as well as liver and renal function (s-ALT, s-creatinine) were measured. All mice survived 48 h after IOI of LHb whereas only 47% and 45% mice survived in 5% albumin and Wash RBC, respectively (Fig. 1 ). The changes in TNF and INFγlevels after IOI were not statistically different among 3 groups (Fig. 2 ) and no side effects were found on liver and renal function.. Conclusions: LHb has a better anti-shock effect than RBC by using IOI probably due to smaller size and IOI of LHb could be useful in disaster medicine. In addition, IOI of LHb shows no significant effects on cytokins, liver and renal function. Figure 1 Figure 2

Chemically modified hemoglobin as an artificial oxygen carrier in dogs with experimental hemorrhagic shock

1991 ◽  
Vol 112 (2) ◽  
pp. 1094-1096
Author(s):  
E. A. Selivanov ◽  
N. I. Kochetygov ◽  
K. A. Gerbut ◽  
M. A. Azhigirova ◽  
E. P. Vyazova ◽  
...  
Keyword(s):  
Hemorrhagic Shock ◽  
Oxygen Carrier ◽  
Artificial Oxygen Carrier ◽  
Chemically Modified ◽  
Experimental Hemorrhagic Shock

Abstract 340: Cardiac Autonomic Activity, Cardiac Function and Arrhythmogenic Property in Heart in Hemorrhagic Shock: Effect of Artificial Oxygen Carrier

Circulation ◽  
2014 ◽  
Vol 130 (suppl_2) ◽  
Author(s):  
Bonpei Takase ◽  
Yoshihiro Tanaka
Keyword(s):  
Cardiac Function ◽  
Hemorrhagic Shock ◽  
Ischemia Reperfusion ◽  
Electrophysiological Study ◽  
Oxygen Carrier ◽  
Conduction Delay ◽  
Autonomic Activity ◽  
Artificial Oxygen Carrier ◽  
Fatty Acid Binding ◽  
Cardiac Autonomic Activity

Lethal ventricular arrhythmias (VT/VF) is serious complications in severe hemorrhagic shock (HS). Cardiac autonomic imbalance and dysfunction are well known unfavorable conditions. However, the effect of oxygenation including artificial oxygen carrier is not fully understood. To investigate the role of oxygenation in lethal HS, cardiac autonomic activity measures, cardiac function test, optical mapping analysis (OMP), and electrophysiological study (EPS) were performed in rats lethal HS. Methods and Results: 1) Over the course of 150 min, rats were subjected to blood withdrawal (0.2 mL/min; total 85% bleeding) and simultaneously transfused with washed rat red blood cells (wRBC), liposome-encapsulated hemoglobin (LHb) or 5% albumin (5%ALB). Temporal changes in cardiac function by 2-D Echocardiography, heart-type fatty acid-binding protein (hFAB) levels, plasma levels of catecholamines, heart rate variability (HRV), and hypoxia-inducible factor 1α expression (HIF1) were measured. As results, > 85% of the rats transfused with either LHb or wRBC survived for 8 days. LHb transfusion suppressed HIF1 expression in the heart, maintained low levels of hFAB, and attenuated sympathetic nerve activity as reflected by changes in HRV and catecholamines. 2) After cannulating 22G catheter into the abdominal aorta, acute HS was induced by withdrawing 30% of total blood for 25 min. After HS, the rats were immediately resuscitated by transfusing the same amount of wRBC, LHb, or 5%ALB. After excising the heart, OMP and EPS were performed in Langendorff-perfused hearts. OMP revealed abnormal ventricular conduction delay in conjunction with impaired action potential duration (APD) dispersion in 5%ALB. In contrast, myocardial conduction velocity and APD dispersion were substantially attenuated in wRBC or LHb. Sustained VT/VF was easily provoked by burst pacing stimulus to the LV in 5%ALB whereas no VT/VF was induced with wRBC or LHb. Conclusions: The results indicate that wRBC and LHb attenuates cardiac dysfunction and sympathetic overactivity during lethal HS. Oxygenated hemoglobin transfusion by using either wRBC or LHb prevents VT/VF by preserving myocardial electrical structures caused by ischemia-reperfusion injury in HS.

A Comparison of the Hemoglobin-Based Oxygen Carrier HBOC-201 to Other Low-Volume Resuscitation Fluids in a Model of Controlled Hemorrhagic Shock

2003 ◽  
Vol 55 (4) ◽  
pp. 747-754 ◽  
Author(s):  
CPT James B. Sampson ◽  
CPT Michael R. Davis ◽  
MAJ Deborah L. Mueller ◽  
LT Vikram S. Kashyap ◽  
LT Donald H. Jenkins ◽  
...  
Keyword(s):  
Hemorrhagic Shock ◽  
Oxygen Carrier ◽  
Volume Resuscitation ◽  
Low Volume

scholarly journals Therapeutic Potentials of an Artificial Oxygen-Carrier, Liposome-Encapsulated Hemoglobin, for Ischemia/Reperfusion-Induced Cerebral Dysfunction in Rats

2010 ◽  
Vol 114 (2) ◽  
pp. 189-197 ◽  
Author(s):  
Jin Kakehata ◽  
Taku Yamaguchi ◽  
Hiroko Togashi ◽  
Ichiro Sakuma ◽  
Hiroshi Otani ◽  
...  
Keyword(s):  
Ischemia Reperfusion ◽  
Oxygen Carrier ◽  
Artificial Oxygen Carrier ◽  
Cerebral Dysfunction

Uridine regulation by the isolated rat liver: perfusion with an artificial oxygen carrier

1982 ◽  
Vol 242 (5) ◽  
pp. R465-R470 ◽  
Author(s):  
A. Monks ◽  
R. L. Cysyk
Keyword(s):  
Rat Liver ◽  
Oxygen Carrier ◽  
Liver Perfusion ◽  
Blood Substitute ◽  
Rat Plasma ◽  
Perfusion Medium ◽  
Artificial Oxygen Carrier ◽  
Isolated Rat Liver ◽  
Rat Liver Perfusion ◽  
Isolated Rat

The isolated rat liver was used to investigate the role of the liver in the regulation of circulating uridine concentrations. A synthetic blood substitute (Fluosol-43) was utilized as an alternative oxygen-carrying perfusion medium to a simplified blood preparation and produced no apparent hepatotoxicity within the perfusion period. The isolated rat liver excreted uridine into a circulating perfusion medium achieving concentrations similar to those found in rat plasma (1.4 +/- 0.6 microM). The mean output of uridine over 2 h was 107 nmol.h-1.g liver-1, but if the perfusate was recirculated the net output of uridine was reduced to 12.7 nmol.h-1.g-1. The rate of depletion of nonphysiological concentrations of circulating uridine was found to be concentration dependent up to 25 microM. At a steady state of circulating uridine, a radioactive uridine spike was cleared with a half-life of 7.4 min and an elimination constant of 0.094 min-1; 30% of the radioactivity appeared in the perfusate as metabolites of uridine within 40 min. Thus the perfused rat liver acts to maintain circulating uridine concentrations similar to those measured in plasma.

Artificial oxygen carrier based on polysaccharides–poly(alkylcyanoacrylates) nanoparticle templates

Biomaterials ◽  
2010 ◽  
Vol 31 (23) ◽  
pp. 6069-6074 ◽  
Author(s):  
Cédric Chauvierre ◽  
Romila Manchanda ◽  
Denis Labarre ◽  
Christine Vauthier ◽  
Michael C. Marden ◽  
...  
Keyword(s):  
Oxygen Carrier ◽  
Artificial Oxygen Carrier

Perflubron Emulsion in Prolonged Hemorrhagic Shock

2003 ◽  
Vol 98 (6) ◽  
pp. 1391-1399 ◽  
Author(s):  
Markus Paxian ◽  
Hauke Rensing ◽  
Katrin Geckeis ◽  
Inge Bauer ◽  
Darius Kubulus ◽  
...  
Keyword(s):  
Gene Expression ◽  
Glutamine Synthetase ◽  
Adenosine Triphosphate ◽  
Oxygen Supply ◽  
Oxygen Carrier ◽  
Oxygen Availability ◽  
Oxygen Carriers ◽  
Hepatocellular Injury ◽  
Artificial Oxygen Carrier ◽  
Stored Blood

Background Liver dysfunction as a result of impaired oxygen availability frequently occurs following hemorrhage and contributes to delayed mortality. Artificial oxygen carriers may improve oxygen supply to vital organs while avoiding the need for allogeneic transfusion. Methods Rats were subjected to hemorrhagic hypotension (mean arterial pressure = 35-40 mmHg for 120 min) and were subsequently resuscitated with (1) stored whole rat blood, (2) pentastarch, or (3) pentastarch combined with perflubron emulsion (PFE; 2.7 or 5.4 g/kg body weight), a second-generation artificial oxygen carrier. Recovery of liver adenosine triphosphate, hepatocellular injury, and expression of glutamine synthetase 1, a gene that is induced by exposure of hepatocytes to low partial pressure of oxygen, were studied at 4 h of resuscitation. Results Stored whole blood or pentastarch failed to restore liver adenosine triphosphate concentrations after prolonged shock as compared to sham controls and resulted in increased gene expression of glutamine synthetase 1. Addition of 2.7 g PFE/kg restored liver adenosine triphosphate to control, whereas 5.4 g PFE/kg resulted in adenosine triphosphate concentrations significantly above control. Improved hepatocellular oxygen supply was also confirmed by restoration of the physiologic expression pattern of glutamine synthetase 1. Serum enzyme concentrations were highest after resuscitation with stored blood, whereas addition of PFE failed to further decrease enzyme concentrations as compared to pentastarch alone. Conclusions Resuscitation with PFE is superior to stored blood or asanguineous resuscitation with respect to restoration of hepatocellular energy metabolism. The improved hepatocellular oxygen availability is reflected in normalization of oxygen-dependent gene expression. However, improved oxygen availability failed to affect early hepatocellular injury.

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