60 IMMATURE OLIGODENDROGLIAL CELLS IN DEVELOPING HUMAN FETAL SPINAL CORD CONTAIN IMMUNOREACTIVE GLIAL FIBRILLARY ACIDIC PROTEIN (GFAP)

AbstractThe glial fibrillary acidic protein (GFAP) is an astrocyte-specific member of the class III intermediate filament proteins. It is generally used as a specific marker of astrocytes in the central nervous system (CNS). We isolated a GFAP cDNA from the brain and spinal cord cDNA library of Gekko japonicus, and prepared polyclonal antibodies against gecko GFAP to provide useful tools for further immunochemistry studies. Both the real-time quantitative PCR and western blot results revealed that the expression of GFAP in the spinal cord after transection increased, reaching its maximum level after 3 days, and then gradually decreased over the rest of the 2 weeks of the experiment. Immunohistochemical analyses demonstrated that the increase in GFAP-positive labeling was restricted to the white matter rather than the gray matter. In particular, a slight increase in the number of GFAP positive star-shaped astrocytes was detected in the ventral and lateral regions of the white matter. Our results indicate that reactive astrogliosis in the gecko spinal cord took place primarily in the white matter during a short time interval, suggesting that the specific astrogliosis evaluated by GFAP expression might be advantageous in spinal cord regeneration.

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Axonal and Nonneuronal Cell Responses to Spinal Cord Injury in Mice Lacking Glial Fibrillary Acidic Protein

Experimental Neurology ◽

10.1006/exnr.1997.6702 ◽

1997 ◽

Vol 148 (2) ◽

pp. 568-576 ◽

Cited By ~ 50

Author(s):

Xia Wang ◽

A. Messing ◽

S. David

Keyword(s):

Spinal Cord ◽

Spinal Cord Injury ◽

Glial Fibrillary Acidic Protein ◽

Acidic Protein ◽

Cell Responses ◽

Cord Injury

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Lentiviral-mediated silencing of glial fibrillary acidic protein and vimentin promotes anatomical plasticity and functional recovery after spinal cord injury

Journal of Neuroscience Research ◽

10.1002/jnr.23468 ◽

2014 ◽

Vol 93 (1) ◽

pp. 43-55 ◽

Cited By ~ 13

Author(s):

Mathieu Desclaux ◽

Florence E. Perrin ◽

Anh Do-Thi ◽

Monica Prieto-Cappellini ◽

Minerva Gimenez y Ribotta ◽

...

Keyword(s):

Spinal Cord ◽

Spinal Cord Injury ◽

Glial Fibrillary Acidic Protein ◽

Functional Recovery ◽

Acidic Protein ◽

Anatomical Plasticity ◽

Cord Injury

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Assessment of Neurone-Specific Enolase, Glial Fibrillary Acidic Protein and S100 B as Spinal Cord Ischemia Biomarkers in Patients Undergoing Open and Endovascular Complex Aortic Surgery: A Single-Center Experience

Annals of Vascular Surgery ◽

10.1016/j.avsg.2020.01.003 ◽

2020 ◽

Vol 66 ◽

pp. 424-433

Author(s):

Alexander Gombert ◽

Marcia V. Rückbeil ◽

Drosos Kotelis ◽

Sara Bürger ◽

Tobias Brugmayer ◽

...

Keyword(s):

Spinal Cord ◽

Glial Fibrillary Acidic Protein ◽

Aortic Surgery ◽

Spinal Cord Ischemia ◽

Acidic Protein ◽

Single Center ◽

Single Center Experience

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Abnormal Reaction to Central Nervous System Injury in Mice Lacking Glial Fibrillary Acidic Protein and Vimentin

The Journal of Cell Biology ◽

10.1083/jcb.145.3.503 ◽

1999 ◽

Vol 145 (3) ◽

pp. 503-514 ◽

Cited By ~ 239

Author(s):

Milos Pekny ◽

Clas B. Johansson ◽

Camilla Eliasson ◽

Josefina Stakeberg ◽

Åsa Wallén ◽

...

Keyword(s):

Spinal Cord ◽

Central Nervous System ◽

Nervous System ◽

Glial Fibrillary Acidic Protein ◽

Intermediate Filament ◽

Glial Scar ◽

Scar Formation ◽

Brain Lesions ◽

Acidic Protein ◽

The Central Nervous System

In response to injury of the central nervous system, astrocytes become reactive and express high levels of the intermediate filament (IF) proteins glial fibrillary acidic protein (GFAP), vimentin, and nestin. We have shown that astrocytes in mice deficient for both GFAP and vimentin (GFAP−/−vim−/−) cannot form IFs even when nestin is expressed and are thus devoid of IFs in their reactive state. Here, we have studied the reaction to injury in the central nervous system in GFAP−/−, vimentin−/−, or GFAP−/−vim−/− mice. Glial scar formation appeared normal after spinal cord or brain lesions in GFAP−/− or vimentin−/− mice, but was impaired in GFAP−/−vim−/− mice that developed less dense scars frequently accompanied by bleeding. These results show that GFAP and vimentin are required for proper glial scar formation in the injured central nervous system and that some degree of functional overlap exists between these IF proteins.

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