DETECTION OF PSP94 AND ITS SPECIFIC BINDING SITES IN THE PROSTATE ADENOCARCINOMA CELL LINE LNCaP

Asialoglycoprotein receptors are highly abundant on the hepatocyte surface and have specific binding sites for blood serum glycoproteins. Such discovery resulted in development of liver-targeted drug delivery systems because modification of the liposomal surface by carbohydrate derivatives results in an increase of endocytosis, which facilitates selective uptake of such systems by hepatocytes. In this study we have synthesized novel lactose derivatives containing a palmitic hydrophobic domain. They were used for modification of the liposome surface. Transfection activity of modified liposomes was analyzed on the HepG2 cell line (hepatocytes) and showed an increase in the transfection efficiency as compared to the non-modified liposomes. At the same time transfection activities of modified and non-modified liposomes were similar in the case of a non-hepatocyte cell line (293T). The novel lactose-based glycoconjugates may be a promising tool for developing efficient vectors for delivery of nucleic acids to hepatocytes.

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Development and characterization of a mouse prostate adenocarcinoma cell line: Ductal formation determined by extracellular matrix

The Prostate ◽

10.1002/(sici)1097-0045(19980101)34:1<10::aid-pros2>3.0.co;2-o ◽

1998 ◽

Vol 34 (1) ◽

pp. 10-22 ◽

Cited By ~ 11

Author(s):

Cheryl L. Jorcyk ◽

Min-Ling Liu ◽

Masa-Aki Shibata ◽

Ioanna G. Maroulakou ◽

Kristin L. Komschlies ◽

...

Keyword(s):

Extracellular Matrix ◽

Cell Line ◽

Prostate Adenocarcinoma ◽

Adenocarcinoma Cell Line ◽

Adenocarcinoma Cell ◽

Mouse Prostate

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Identification and characterization of opioid-binding sites present in the Ishikawa human endometrial adenocarcinoma cell line

The Journal of Clinical Endocrinology & Metabolism ◽

10.1210/jc.80.2.418 ◽

1995 ◽

Vol 80 (2) ◽

pp. 418-423 ◽

Cited By ~ 9

Author(s):

A. Hatzoglou

Keyword(s):

Cell Line ◽

Binding Sites ◽

Endometrial Adenocarcinoma ◽

Adenocarcinoma Cell Line ◽

Adenocarcinoma Cell ◽

Opioid Binding ◽

Identification And Characterization

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Characterization of human relaxin gene regulation in the relaxin-expressing human prostate adenocarcinoma cell line LNCaP.FGC

Journal of Molecular Endocrinology ◽

10.1677/jme.0.0150153 ◽

1995 ◽

Vol 15 (2) ◽

pp. 153-166 ◽

Cited By ~ 20

Author(s):

J M Gunnersen ◽

P J Roche ◽

G W Tregear ◽

R J Crawford

Keyword(s):

Cell Line ◽

Reporter Gene ◽

Seminal Fluid ◽

Prostate Gland ◽

Prostate Adenocarcinoma ◽

Gene Promoters ◽

Adenocarcinoma Cell Line ◽

Prostate Cell ◽

Adenocarcinoma Cell ◽

Flanking Regions

ABSTRACT Relaxin is a peptide hormone which is produced in human reproductive tissues including the ovary and prostate gland. Little is known of the molecular events regulating relaxin gene transcription. We have studied this question using gene transfer of relaxin promoter/reporter gene constructs into a relaxin-expressing cell line. A number of human cell lines expressed relaxin as detected by reverse transcription-PCR. In one of these lines, the prostate adenocarcinoma cell line LNCaP.FGC, relaxin mRNA was also detected by Northern blot analysis. The DNA sequences of the proximal 5′-flanking regions (∼900 nucleotides) of the two human relaxin genes, HI and H2, were determined. Deletion constructs containing portions of the 5′-flanking regions of HI and H2 linked to the bacterial chloramphenicol acetyl transferase reporter gene were prepared. The expression of the reporter gene constructs was analysed in the LNCaP.FGC cell line and the results of these transient transfection assays have led to the identification of positive and negative regulatory regions within the 5′-flanking DNA. A difference in activity of the H1 and H2 gene promoters in this prostate cell line was observed, with the H2 promoter being more active. This situation may mimic that occurring in vivo since the relaxin secreted from the prostate gland into seminal fluid is the product of the H2 gene.

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