IMMUNOLOGICAL DETERMINATION OF PITUITARY LUTEINIZING HORMONE IN THE URINE OF FERTILE AND POST-MENOPAUSAL WOMEN AND ADULT MEN

1962 ◽  
Vol 17 (6) ◽  
pp. 845-846
Author(s):  
LEIF WIDE ◽  
CARL GEMZELL
Keyword(s):  
Luteinizing Hormone ◽  
Adult Men ◽  
Menopausal Women ◽  
Post Menopausal

IMMUNOLOGICAL DETERMINATION OF PITUITARY LUTEINIZING HORMONE IN THE URINE OF FERTILE AND POST-MENOPAUSAL WOMEN AND ADULT MEN

1962 ◽  
Vol 39 (4) ◽  
pp. 539-546 ◽  
Author(s):  
Leif Wide ◽  
Carl Gemzell
Keyword(s):  
Luteinizing Hormone ◽  
Haemagglutination Inhibition ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Human Pituitary ◽  
Adult Men ◽  
Menopausal Women ◽  
Post Menopausal ◽  
Inhibition Reaction

ABSTRACT An immunological method to assay human pituitary luteinizing hormone (HPLH) in urine is described. It is based on the fact that HPLH crossreacts with human chorionic gonadotrophin (HCG) in an haemagglutination inhibition reaction between HCG-coated blood cells and rabbit HCG-antisera. During the menstrual cycle the excretion of HPLH reached a peak of 200–400 U per liter at the time of ovulation. In the urine of post-menopausal women the concentration of HPLH was between 100 and 400 U per liter. In the urine of adult men the concentration of HPLH was between 50 and 160 U per liter.

RADIOIMMUNOASSAY FOR 2-METHOXYOESTRONE IN HUMAN PLASMA

1979 ◽  
Vol 91 (1) ◽  
pp. 158-166 ◽  
Author(s):  
Günter Emons ◽  
Peter Ball ◽  
Gertrud v. Postel ◽  
Rudolf Knuppen
Keyword(s):  
Human Plasma ◽  
Plasma Concentrations ◽  
Cross Reactivity ◽  
Elderly Men ◽  
Specific Antibodies ◽  
Assay Procedure ◽  
Menopausal Women ◽  
Bovine Serum Albumin Conjugate ◽  
Post Menopausal

ABSTRACT A bovine serum albumin conjugate of 2-methoxyoestrone was used for the preparation of highly specific antibodies in rabbits. Cross-reactivity for catecholoestrogens and monophenolic steroids was below 0.3 %. Only 2-methoxyoestradiol cross-reacted with 44 %. An assay procedure for the determination of unconjugated and conjugated 2-methoxyoestrone in human plasma is described. The following mean plasma concentrations (pg/ml) were found (unconjugated/conjugated): children 61/1130, young men 74/1320, elderly men 109/1260, cycling women 131/1040, post-menopausal women 102/1420, and pregnant women 3980/5850.

Glycerol prevents loss of immunoreactive follicle-stimulating hormone and luteinizing hormone from frozen urine

1983 ◽  
Vol 98 (3) ◽  
pp. 381-384 ◽  
Author(s):  
J. H. Livesey ◽  
H. K. Roud ◽  
M. G. Metcalf ◽  
R. A. Donald
Keyword(s):  
Luteinizing Hormone ◽  
Follicle Stimulating Hormone ◽  
Urine Samples ◽  
Initial Value ◽  
Menopausal Women ◽  
Post Menopausal ◽  
Stimulating Hormone

First morning urine samples were collected from both menstruant and post-menopausal women and stored at −25 °C. Immunoreactive FSH disappeared from these samples (t½ = 30 days), ultimately stabilizing at about 20% of the initial value. The loss was more rapid at −20 °C and less rapid at −55 °C and +4°C. Immunoreactive LH was also lost from frozen urine, but more slowly than FSH. The addition of glycerol to urine (0·52 mol/l) stored at −25 °C prevented loss of immunoreactive FSH and LH for at least 105 days.

Abstract 15572: Trans Fat Consumption is Adversely Linked to Memory in Working-Age Adults

Circulation ◽  
2014 ◽  
Vol 130 (suppl_2) ◽  
Author(s):  
Beatrice A Golomb ◽  
Alexis K Bui
Keyword(s):  
Cross Sectional ◽  
Full Sample ◽  
Adult Men ◽  
Word Memory ◽  
Fat Consumption ◽  
Menopausal Women ◽  
Working Age ◽  
Cell Energy ◽  
Post Menopausal ◽  
The Relationship

Background: Dietary industrial trans fatty acids ( dTFA ) have pro oxidant and cell energy harms . Food with anti oxidant and cell energy benefits has been favorably linked to word memory in younger adults, prior to major age-induced variance. Goal: We assessed the cross-sectional relation of dTFA to word-memory. Participants: 1018 adult men age ≥20 and post-menopausal women without CAD, with LDL 115-190mg/dL, fasting glucose ≤142mg/dL, assessed in 1999-2004 (before dTFA regulation). Primary analyses focused on men, as only men (N=694) were represented in younger adult ages. Measurements: “Recurrent words” ( RecWd ) assessed word memory. A Food Frequency Questionnaire ( FFQ ) estimated TFA (gms/day). An age-by-dTFA interaction was assessed. Regression, stratified at age 45, assessed the relation between RecWd and dTFA across models adjusting for potential confounders (see Table). Potential mediators were examined. Results: An age-by-dTFA interaction was significant (P=0.025), supporting stratification. dTFA adversely predicted memory in age <45 (only), robust to adjustment model (Table). Findings were replicated in the full sample, including women (not shown). Each gm/day dTFA was associated with an estimated 0.76 fewer words recalled (full model), P=0.006 - yielding an estimated 11 fewer words at the highest dTFA intake vs none - from a mean of 86. Adjustment for SBP, waist and BMI (but not lipid or glycemic variables) attenuated the relationship, consistent with mediation by factors involving, relating to, or concurrently influencing, these factors. Conclusion: Greater dTFA was significantly associated with worse word memory in adults aged 20-45 years, often critical years for career building. dTFA findings remain relevant because US regulations do not extend worldwide, and presumed dTFA mechanisms have relevance to other exposures. Prooxidant and energetic detriments of dTFA and triangulation with other evidence offer prospects for causality.

DETERMINATION OF TAMOXIFEN AND AN HYDROXYLATED METABOLITE IN PLASMA FROM PATIENTS WITH ADVANCED BREAST CANCER USING GAS CHROMATOGRAPHY–MASS SPECTROMETRY

1979 ◽  
Vol 83 (3) ◽  
pp. 401-408 ◽  
Author(s):  
C. P. DANIEL ◽  
S. J. GASKELL ◽  
H. BISHOP ◽  
R. I. NICHOLSON
Keyword(s):  
Breast Cancer ◽  
Advanced Breast Cancer ◽  
Tamoxifen Therapy ◽  
Advanced Breast ◽  
Gas Chromatography Mass Spectrometry ◽  
Menopausal Women ◽  
Considerable Excess ◽  
Post Menopausal ◽  
Hydroxylated Metabolite

An assay has been established for the selective measurement of tamoxifen and its monohydroxy derivative, metabolite B, in human plasma. The assay was used to examine the concentrations of these compounds, relative to oestradiol-17β, in the plasma of patients undergoing tamoxifen therapy for advanced breast cancer. Oral administration of the drug (20 mg twice a day) raised the level of tamoxifen in plasma to approximately 200 ng/ml 20 days after the commencement of treatment. This level was 3000-fold higher than the corresponding concentration of oestradiol which remained within the range for post-menopausal women. Metabolite B was present in plasma at a much lower concentration than tamoxifen although in considerable excess over oestradiol. The overall results are discussed in relation to the possible mechanism of action of the drug.

THE SPECIFICITY AND APPLICATION OF A RADIOIMMUNOASSAY FOR THE α-SUBUNIT OF LUTEINIZING HORMONE IN MAN

1975 ◽  
Vol 78 (4) ◽  
pp. 664-674 ◽  
Author(s):  
C. Hagen ◽  
A. S. McNeilly
Keyword(s):  
Luteinizing Hormone ◽  
Cord Blood ◽  
Glycoprotein Hormones ◽  
Α Subunit ◽  
Menopausal Women ◽  
Post Menopausal ◽  
Lh Rh ◽  
Circulating Levels

ABSTRACT A sensitive radioimmunoassay for the α-subunit of LH has been developed. Differences in the immunopotency of LH, FSH and subunit preparations were found and are described in detail. The assay has been applied to the measurement of circulating levels of α-subunit; higher levels of α-subunit were found in post-menopausal than in pre-menopausal women, or men, and a rise in α-subunit was seen after LH-RH but not TRH administration. Elevated levels of free α-subunit were found in foetal cord blood at term. These results suggest that the α-subunit circulates independently of the intact pituitary and placental glycoprotein hormones.

The effect in post-menopausal women of natural human and artificial oestrogens on the concentration in serum of prolactin

1980 ◽  
Vol 95 (4) ◽  
pp. 433-437 ◽  
Author(s):  
Jesper Rye Andersen ◽  
Erik Schroeder ◽  
Paul Erik Lebech
Keyword(s):  
Luteinizing Hormone ◽  
Serum Concentration ◽  
Follicle Stimulating Hormone ◽  
Dose Dependence ◽  
Blood Samples ◽  
Younger Women ◽  
Menopausal Women ◽  
Post Menopausal ◽  
Higher Doses ◽  
Stimulating Hormone

Abstract. 25 women aged 53–78 years with at least two vears menopause were divided in three groups receiving one of the following oral treatments for three weeks: 2 mg oestradiol-17β plus 1 mg oestriol, 4 mg oestradiol-17β plus 2 mg oestriol or 50 μg ethinyloestradiol daily. Blood samples were collected before, during and after the treatment and the effect on the serum concentration of prolactin, oestradiol-17β, follicle-stimulating hormone and luteinizing hormone was evaluated. During treatment with natural human oestrogens serum oestradiol-17β levels were significantly higher than before treatment. The serum concentration of prolactin was unchanged in patients receiving 2 mg oestradiol-17β plus 1 mg oestriol but increased in patients receiving 4 mg oestradiol-17β plus 2 mg oestriol or 50 μg ethinyloestradiol, thus indicating dose-dependence for natural human oestrogens. However, the increase was moderate, and these higher levels were not significantly different from levels of prolactin in serum found in 16 younger women. Concentrations of follicle-stimulating hormone and luteinizing hormon were depressed during treatment, the former to significantly lower levels when higher doses of oestrogens were used.

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