EFFICACY OF SHORT COURSES OF ORAL NOVOBIOCIN-RIFAMPIN IN ERADICATING CARRIER STATE OF METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS AND IN VITRO KILLING STUDIES OF CLINICAL ISOLATES

Abstract This descriptive cross-sectional study was performed in the Department of Microbiology, Fauji Foundation Hospital, Rawalpindi, from March 2019 to September 2019 to determine the in vitro efficacy of Daptomycin against clinical isolates of Methicillin-Resistant Staphylococcus aureus (MRSA). Consecutive non-probability sampling technique was used and a total number of 270 patients’ Pan Cultures having MRSA growth on Cefoxatin Disc with size less than 22 mm zone size were included in the study. Cultures were inoculated on MacConkey, Chocolate and Blood agar and then incubated for 24 hours at 37 degree Celsius. After incubation, Coagulase test, Catalase test and Gram staining technique were used for further identification. Minimum Inhibitory Concentration (MIC) of the isolates for Daptomycin was obtained by using E strips (Oxoid UK) according to Clinical & Laboratory Standards Institute (CLSI) guidelines. Continuous...

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In Vitro Antimicrobial Activity of Telavancin against Methicillin-resistant Staphylococcus aureus Clinical Isolates from Japan (2006)

The Journal of Antibiotics ◽

10.1038/ja.2007.91 ◽

2007 ◽

Vol 60 (11) ◽

pp. 709-712 ◽

Cited By ~ 5

Author(s):

Kazuo Hatano ◽

Kaoru Matsuzaki ◽

Yumie Sato ◽

Intetsu Kobayashi ◽

Keizo Yamaguchi

Keyword(s):

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Methicillin Resistant Staphylococcus Aureus ◽

Clinical Isolates ◽

Methicillin Resistant ◽

In Vitro Antimicrobial Activity

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In-Vitro Antibacterial Activity of crude Garlic (Allium Sativum) Extract Against Clinical Isolates of Methicillin Resistant Staphylococcus aureus

Biomedical & Pharmacology Journal ◽

10.13005/bpj/2247 ◽

2021 ◽

Vol 14 (3) ◽

pp. 1449-1457

Author(s):

Salma Osman Noorelhuda Mohammed ◽

Nadir Musa Khalil Abuzeid ◽

Sara Abdelghani ◽

Lienda Bashier Eltayeb

Keyword(s):

Staphylococcus Aureus ◽

Allium Sativum ◽

Antimicrobial Agents ◽

Methicillin Resistant Staphylococcus Aureus ◽

Clinical Isolates ◽

Diffusion Method ◽

Negative Consequences ◽

Methicillin Resistant ◽

And Staphylococcus Aureus

Background:Methicillin-resistant Staphylococcus aureus (MRSA) has gained significant health solicitude globallydue to its resistance to nearly almost antimicrobial agents, and garlic is one of nature's most powerful antibiotics that must be used as a pharmaceutical regimen. The current study aimed to determine the In-Vitro antibacterial efficacy of crude garlic extract against MRSA. Methods: The aqueous and 70% ethanol crude garlic (Alllium sativum)extract was prepared. Disc diffusion method was performed to assess the antimicrobial activity for100 clinical isolates of MRSA collected,The reference standard strain was Staphylococcus aureus (ATCC 25923). Results: All MRSA strains assessed were significantly sensitive to 70% ethanolic extract at various concentrations range from 200 to 25%, exhibited inhibitory effects against clinical isolates and Staphylococcus aureus (ATCC 25923) with the means of inhibition zones ranging from 17.76- 14.35 mm and 15-13 mm in length, while the aqueous extracts were less in both clinical isolates and Staphylococcus aureus (ATCC 25923) ranging from 11.93-8.62 mm and 11-8 mm respectively, methanol and distilled water were not effected on growth. Conclusion: The findings demonstrate that 70%ethanol extract of crude Allium sativum has significantly inhibitory effect on methicillin-resistant Staphylococcus aureus is better than aqueous extract. This study does not undermine the value of antibiotic use, but instead the probability of using them in low dosage to minimize their negative consequences.

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