Maarten, G., et al. Wound healing of the palatal mucoperiosteum in beagle dogs after surgery at different ages. J. Craniomaxillofac. Surg. 15

Objective : Biomodification of the root surface plays a major role in periodontal wound healing. Root surface modification with bone morphogenetic protein (BMP) stimulates bone and cementum-like tissue formation; however, severe ankylosis is simultaneously observed. Bio-safe collagen hydrogel scaffolds may therefore be useful for supplying periodontal ligament cells and preventing ankylosis. We examined the effects of BMP modification in conjunction with collagen hydrogel scaffold implantation on periodontal wound healing in dogs. Material and Methods: The collagen hydrogel scaffold was composed of type I collagen sponge and collagen hydrogel. One-wall infrabony defects (5 mm in depth, 3 mm in width) were surgically created in six beagle dogs. In the BMP/Col group, BMP-2 was applied to the root surface (loading dose; 1 µg/µl), and the defects were filled with collagen hydrogel scaffold. In the BMP or Col group, BMP-2 coating or scaffold implantation was performed. Histometric parameters were evaluated at 4 weeks after surgery. Results: Single use of BMP stimulated formation of alveolar bone and ankylosis. In contrast, the BMP/Col group frequently enhanced reconstruction of periodontal attachment including cementum-like tissue, periodontal ligament and alveolar bone. The amount of new periodontal ligament in the BMP/Col group was significantly greater when compared to all other groups. In addition, ankylosis was rarely observed in the BMP/Col group. Conclusion: The combination method using root surface modification with BMP and collagen hydrogel scaffold implantation facilitated the reestablishment of periodontal attachment. BMP-related ankylosis was suppressed by implantation of collagen hydrogel.

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Growth of the maxilla after soft tissue palatal surgery at different ages in beagle dogs: A longitudinal radiographic study

Journal of Oral and Maxillofacial Surgery ◽

10.1016/0278-2391(88)90084-5 ◽

1988 ◽

Vol 46 (3) ◽

pp. 204-209 ◽

Cited By ~ 14

Author(s):

Maarten G.M.M. Wijdeveld ◽

Ellen M. Grupping ◽

Anne M. Kuijpers-Jagtman ◽

Jaap C. Maltha

Keyword(s):

Soft Tissue ◽

Radiographic Study ◽

Beagle Dogs ◽

Different Ages ◽

Palatal Surgery

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Wound Healing in Beagle Dogs after Palatal Repair without Denudation of Bone

The Cleft Palate-Craniofacial Journal ◽

10.1597/1545-1569_1995_032_0363_whibda_2.3.co_2 ◽

1995 ◽

Vol 32 (5) ◽

pp. 363-370 ◽

Cited By ~ 4

Author(s):

Thomas S. Leenstra ◽

Jaap C. Maltha ◽

Anne Marie Kuijpers-Jagtman ◽

Paul H.M. Spauwen

Keyword(s):

Wound Healing ◽

Scar Tissue ◽

Control Group ◽

Beagle Dogs ◽

Flap Technique ◽

Group 2 ◽

Palatal Bone ◽

Lamellar Type ◽

Palatal Surgery ◽

Group 1

The connection of scar tissue to palatal bone by Sharpey's fibers, after cleft palate surgery, might lead to maxillary growth inhibition. The aim of this study, in beagle dogs, was to investigate the possibility of preventing the development of Sharpey's fibers by means of a modified surgical technique. In group 1, palatal repair according to von Langenbeck, was simulated. In group 2, palatal surgery was performed using a new partially split flap technique. The palates were histologically evaluated 12 weeks after surgery and compared with a control group. In group 1, the scar tissue was firmly attached to bundle bone by means of Sharpey's fibers. In group 2 and in the control group, this kind of attachment was not found; the bone was of the lamellar type. The partially split flap technique had led to the development of vaguely demarcated scar tissue and it had prevented, to a large extent, development of Sharpey's fibers.

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Effects of periodontal dressings on fibroblasts and gingival wound healing in dogs

Acta Veterinaria Hungarica ◽

10.1556/avet.52.2004.1.4 ◽

2004 ◽

Vol 52 (1) ◽

pp. 33-46 ◽

Cited By ~ 4

Author(s):

M. Petelin ◽

Z. Pavlica ◽

Urška Batista ◽

Draga Štiblar-Martinčič ◽

U. Skalerič

Keyword(s):

Cell Proliferation ◽

Wound Healing ◽

Inflammatory Reaction ◽

Inhibitory Effect ◽

The Other ◽

Gingival Tissue ◽

Histological Evaluation ◽

Beagle Dogs ◽

Inhibitory Effects

In the present study the effects of different commercially available periodontal dressings (Peripac, Barricaid, Fittydent, Reso-Pack and Myzotect-tincture) on fibroblast (V-79-379A) proliferation and survival were tested in vitro. Barricaid, Fittydent and Reso-Pack periodontal dressings have only small inhibitory effects on cell proliferation (83.3 ± 9%, 71.6 ± 8.7% and 87.3 ± 4.5% of control after 48 h, respectively) in comparison with the great inhibitory effect of Myzotect-tincture (2.9 ± 0.1%) and Peripac (33.7 ± 11.4%) (p < 0.001). Barricaid was the only dressing where 41% of cells survived after exposure, while the other four dressings killed all the cells in 6 days. In addition, the healing of artificially created gingival wounds covered by Barricaid and Reso-Pack was followed for 7 days in 12 Beagle dogs. Histological evaluation of gingival tissue demonstrated that wounds covered by Reso-Pack showed the best epithelisation and vascularity and the least inflammatory reaction in first 4days. Later the observed parameters were similar with those of wounds covered by Barricaid or without pack. The present results indicate that Peripac periodontal dressing and Myzotect-tincture showed the highest cytotoxicity to fibroblasts in vitro. From the histological observations in Beagle dogs Reso-Pack has been found to be the most suitable dressing, followed by Barricaid.

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