Synthesis of a Kunitz-Type Serine Proteinase Inhibitory Protein That Shares Homology with Bovine Pancreatic Trypsin Inhibitor by Ovine Intervertebral Disc Cells in Serum-Free Alginate Bead Culture

2002 ◽  
Vol 15 (2) ◽  
pp. 164-171 ◽  
Author(s):  
James Melrose ◽  
Susan Smith ◽  
Peter Ghosh
Keyword(s):  
Intervertebral Disc ◽  
Trypsin Inhibitor ◽  
Alginate Bead ◽  
Serine Proteinase ◽  
Bovine Pancreatic Trypsin Inhibitor ◽  
Inhibitory Protein ◽  
Serum Free ◽  
Pancreatic Trypsin Inhibitor ◽  
Disc Cells ◽  
Kunitz Type

Genes encoding WFDC- and Kunitz-type protease inhibitor domains: are they related?

2011 ◽  
Vol 39 (5) ◽  
pp. 1398-1402 ◽  
Author(s):  
Åke Lundwall ◽  
Adam Clauss
Keyword(s):  
Human Chromosome ◽  
Protease Inhibitor ◽  
Protease Inhibitors ◽  
Trypsin Inhibitor ◽  
Bovine Pancreatic Trypsin Inhibitor ◽  
Sequence Motif ◽  
Genes Encoding ◽  
Pancreatic Trypsin Inhibitor ◽  
Kunitz Type ◽  
Kunitz Type Protease Inhibitor

We have previously demonstrated that the genes of SCPs (semen coagulum proteins) and the WFDC (whey acidic protein four-disulfide core)-type protease inhibitor elafin are homologous in spite of lacking similarity between their protein products. This led to the discovery of a locus on human chromosome 20, encompassing genes of the SCPs, SEMG1 (semenogelin I) and SEMG2, and 14 genes containing the sequence motif that is characteristic of WFDC-type protease inhibitors. We have now identified additional genes at the locus that are similarly organized, but which give rise to proteins containing the motif of Kunitz-type protease inhibitors. Here, we discuss the evolution of genes encoding SCPs and describe mechanisms by which they and genes with Kunitz motifs might have evolved from genes with WFDC motifs. We can also demonstrate an expansion of the WFDC locus with 0.6 Mb in the cow. The region, which seems to be specific to ruminants, contains several genes and pseudogenes with Kunitz motifs, one of which is the much-studied BPTI (bovine pancreatic trypsin inhibitor).

scholarly journals Screening for Stable Mutants with Amino Acid Pairs Substituted for the Disulfide Bond between Residues 14 and 38 of Bovine Pancreatic Trypsin Inhibitor (BPTI)

2002 ◽  
Vol 277 (52) ◽  
pp. 51043-51048 ◽  
Author(s):  
Yoshihisa Hagihara ◽  
Kentaro Shiraki ◽  
Tsutomu Nakamura ◽  
Koichi Uegaki ◽  
Masahiro Takagi ◽  
...  
Keyword(s):  
Amino Acid ◽  
Disulfide Bond ◽  
Trypsin Inhibitor ◽  
Bovine Pancreatic Trypsin Inhibitor ◽  
Pancreatic Trypsin Inhibitor

Synthetic Approaches to Disulfide-free Circular Bovine Pancreatic Trypsin Inhibitor (c-BPTI) Analogues

2006 ◽  
Vol 12 (2) ◽  
pp. 93-104 ◽  
Author(s):  
Judit Tulla-Puche ◽  
Irina V. Getun ◽  
Yvonne M. Angell ◽  
Jordi Alsina ◽  
Fernando Albericio ◽  
...  
Keyword(s):  
Trypsin Inhibitor ◽  
Bovine Pancreatic Trypsin Inhibitor ◽  
Pancreatic Trypsin Inhibitor ◽  
Synthetic Approaches

A Pulsed-Field Gradient NMR Study of Bovine Pancreatic Trypsin Inhibitor Self-Association†

Biochemistry ◽  
1997 ◽  
Vol 36 (11) ◽  
pp. 3383-3388 ◽  
Author(s):  
Elena Ilyina ◽  
Vikram Roongta ◽  
Hong Pan ◽  
Clare Woodward ◽  
Kevin H. Mayo
Keyword(s):  
Field Gradient ◽  
Trypsin Inhibitor ◽  
Bovine Pancreatic Trypsin Inhibitor ◽  
Pulsed Field Gradient Nmr ◽  
Pulsed Field ◽  
Nmr Study ◽  
Pancreatic Trypsin Inhibitor ◽  
Self Association
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