Preimplantation Genetic Testing for Aneuploidy Versus Morphology as Selection Criteria for Single Frozen-Thawed Embryo Transfer in Good-Prognosis Patients

Abstract Background: Displacement of the window of implantation (WOI) has been proposed as an important factor contributing to RIF. However, histologic dating of the endometrium as a diagnostic tool of endometrial receptivity has been questioned. Methods: This is a prospective intervention trial that entailed 205 infertile patients from July 2017 to December 2017. Endometrial biopsies from 50 good-prognosis patients were conducted on day 3 (n=6), 5 (n=6), 7 (n=26), 9 (n=6) or 11 (n=6) post-ovulation (PO+3/5/7/9/11) of the previous natural cycle before their conventional frozen-thawed embryo transfer (FET) cycle. We conducted endometrial biopsies of 155 RIF patients on day PO +7. Results: The verification of the Noyes criteria for endometrial dating was conducted at different times (PO +3/+5/+7/+9/+11) on 41 good-prognosis patients who achieved an ongoing pregnancy in their first conventional FET cycle after endometrial biopsy. The agreement between two pathologists for endometrial biopsy dating in infertile patients was determined to be acceptable (weighted kappa = 0.672, P < 0.001). The rate of out-of-phase dating on day PO+7 was significantly higher in RIF patients than good- prognosis patients (31.6% vs . 3.8%, P=0.003). pFET was performed in 47 RIF patients diagnosed to be out of phase, and the cumulative live-birth rate was 61.7%. Conclusions: Histologic endometrial dating of RIF patients in natural cycles may be a biomarker for a receptive endometrium in diagnosing the displacement of WOI.

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Clinical value of endometrial histological dating for personalized frozen-thawed embryo transfer (pFET) in patients with repeated implantation failure (RIF) in natural cycle

10.21203/rs.2.16202/v1 ◽

2019 ◽

Author(s):

Yuan Li ◽

Xiaofeng Li ◽

Jingnan liao ◽

Xiangxiu Fan ◽

Yongbin Hu ◽

...

Keyword(s):

Embryo Transfer ◽

Birth Rate ◽

Live Birth Rate ◽

Weighted Kappa ◽

Good Prognosis ◽

Natural Cycle ◽

Clinical Value ◽

Infertile Patients ◽

Frozen Thawed Embryo Transfer ◽

Thawed Embryo Transfer

Abstract Background: The displacement of window of implantation (WOI) has been proposed as an important factor contributed to RIF. However, endometrial histological dating as the diagnostic tool of endometrial receptivity has been questioned. Methods : This is a prospective intervention trial including 205 infertile patients from July 2017 to December 2017.Endometrial biopsies from 50 good prognosis patients were conducted on day 3(n=6), 5(n=6), 7(n=26), 9(n=6) or 11(n=6) of post-ovulation (PO+3/5/7/9/11) in the previous natural cycle before their conventional frozen-thawed embryo transfer (FET) cycle. The endometrial biopsies of 155 RIF patients were conducted on day of PO +7. Results: The verification of Noyes criterion for endometrial dating was conducted in different time (PO +3/+5/+7/+9/+11) from 41 good prognosis patients achieving ongoing pregnancy in the first conventional FET cycle after the endometrial biopsies. The agreement between two pathologists for endometrium dating in infertile patients was determined to be good (weighted kappa = 0.672; P < 0.001). The rate of out-of-phase dating on the day of PO+7 was significantly higher in RIF patients than good prognosis patients (31.6% vs 3.8%, P=0.003).pFET was performed in 47 RIF patients diagnosed to be out of phase, and the accumulative live birth rate was 55.7%. Conclusions: The endometrial histological dating in RIF patients in natural cycle may be a endometrial receptive biomarker for diagnosing the displacement of WOI.

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Clinical value of histologic dating of the endometrium for personalized frozen-thawed embryo transfer (pFET) in patients with repeated implantation failure (RIF) in natural cycles

10.21203/rs.2.16202/v2 ◽

2019 ◽

Author(s):

Yuan Li ◽

Xiaofeng Li ◽

Jingnan liao ◽

Xiangxiu Fan ◽

Yongbin Hu ◽

...

Keyword(s):

Embryo Transfer ◽

Live Birth Rate ◽

Good Prognosis ◽

Endometrial Biopsy ◽

Clinical Value ◽

Cumulative Live Birth Rate ◽

Infertile Patients ◽

Frozen Thawed Embryo Transfer ◽

Natural Cycles ◽

Thawed Embryo Transfer

Abstract Background: Displacement of the window of implantation (WOI) has been proposed as an important factor contributing to RIF. However, histologic dating of the endometrium as a diagnostic tool of endometrial receptivity has been questioned. Methods: This is a prospective intervention trial that entailed 205 infertile patients from July 2017 to December 2017. Endometrial biopsies from 50 good-prognosis patients were conducted on day 3 (n=6), 5 (n=6), 7 (n=26), 9 (n=6) or 11 (n=6) post-ovulation (PO+3/5/7/9/11) of the previous natural cycle before their conventional frozen-thawed embryo transfer (FET) cycle. We conducted endometrial biopsies of 155 RIF patients on day PO +7. Results: The verification of the Noyes criteria for endometrial dating was conducted at different times (PO +3/+5/+7/+9/+11) on 41 good-prognosis patients who achieved an ongoing pregnancy in their first conventional FET cycle after endometrial biopsy. The agreement between two pathologists for endometrial biopsy dating in infertile patients was determined to be acceptable (weighted kappa = 0.672, P < 0.001). The rate of out-of-phase dating on day PO+7 was significantly higher in RIF patients than good- prognosis patients (31.6% vs . 3.8%, P=0.003). pFET was performed in 47 RIF patients diagnosed to be out of phase, and the cumulative live-birth rate was 55.7%. Conclusions: Histologic endometrial dating of RIF patients in natural cycles may be a biomarker for a receptive endometrium in diagnosing the displacement of WOI.

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Comparison of birthweights in patients randomly assigned to fresh or frozen-thawed embryo transfer

10.26226/morressier.573c1513d462b80296c98a23 ◽

2016 ◽

Author(s):

Bruce Shapiro

Keyword(s):

Embryo Transfer ◽

Frozen Thawed Embryo Transfer ◽

Thawed Embryo Transfer

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THE EFFECT OF ASSISTED HATCHING IN FROZEN-THAWED EMBRYO TRANSFER CYCLES

Journal of Medicine and Pharmacy ◽

10.34071/jmp.2016.3.12 ◽

2016 ◽

pp. 80-84

Author(s):

Thi Tam An Nguyen ◽

Minh Tam Le ◽

Ngoc Thanh Cao

Keyword(s):

Embryo Transfer ◽

Zona Pellucida ◽

Assisted Hatching ◽

Implantation Failure ◽

Multiple Pregnancies ◽

Recurrent Implantation Failure ◽

Key Words Laser ◽

Implantation And Pregnancy Rates ◽

Frozen Thawed Embryo Transfer ◽

Thawed Embryo Transfer

Background: Laser assisted hatching technique based on the hypothesis to make an artificial hole on zona pellucida (ZP) that can help embryo hatching out of ZP easily. This technique has been shown to increase implantation and pregnancy rates in women of advanced age, in women with recurrent implantation failure and following the transfer of frozen–thawed embryos. This study described the outcome of frozen–thawed embryo transfers with laser assisted hatching (LAH), which is one of the safest method in nowadays. Purpose: To assess the effect of assisted hatching technique on the clinical outcomes in vitrified-warmed transfer cycles. Method: A total of 65 thawed-transfer cycles with 153 thawed-embryos undertaken within a 12-month period were analysed, Assisted hatching with laser zona thinning was performed with one-quarter of the zona pellucida circumference. The overall thawed-embryos (day 3) were kept in culture overnight. Patient were prepared the suitable endometrium and transferred embryos advantageously. Results: In which, having the rate of survival embryos were 143 occupying 94.3%, the percentage of grade 1 and 2 embryos occupied 55.9% and 29,3% respectively, and that were enrolled LAH before transfering of frozen–thawed embryos. The average transferred embryos were 2.4±0.8, The rate of implantation per transferred embryos and per transferred embryos cycles was 19.5% and 43.1% respectively. The rate of clinical pregnancies per embryo transfer cycles occupied 33.8% with percentage of early miscarriages (biochemical pregnancies and early clinical miscarriages) was 12.3%. The rate of ongoing pregnancies was 30.8% and multiple pregnancies was low just 12.3%. This result was equal or higher than other researchs in embryos transfer had or no LAH. Conclusion: LAH contributed to stable frozen–thawed embryos transfer effectiveness. Key words: Laser assisted hatching, frozen–thawed embryos transfer, zona pellucida (ZP)

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The effects of the day of trophectoderm biopsy and blastocyst grade on the clinical and neonatal outcomes of preimplantation genetic testing–frozen embryo transfer cycles

Zygote ◽

10.1017/s0967199421000435 ◽

2021 ◽

pp. 1-6

Author(s):

Linjun Chen ◽

Zhenyu Diao ◽

Jie Wang ◽

Zhipeng Xu ◽

Ningyuan Zhang ◽

...

Keyword(s):

Birth Weight ◽

Genetic Testing ◽

Embryo Transfer ◽

Live Birth ◽

Frozen Embryo Transfer ◽

Neonatal Outcomes ◽

Miscarriage Rate ◽

Birth Rates ◽

Preimplantation Genetic Testing ◽

Live Birth Rates

Summary This study analyzed the effects of the day of trophectoderm (TE) biopsy and blastocyst grade on clinical and neonatal outcomes. The results showed that the implantation and live birth rates of day 5 (D5) TE biopsy were significantly higher compared with those of D6 TE biopsy. The miscarriage rate of the former was lower than that of the latter, but there was no statistically significant difference. Higher quality blastocysts can achieve better implantation and live birth rates. Among good quality blastocysts, the implantation and live birth rates of D5 and D6 TE biopsy were not significantly different. Among fair quality and poor quality blastocysts, the implantation and live birth rates of D5 TE biopsy were significantly higher compared with those of D6 TE biopsy. Neither blastocyst grade nor the day of TE biopsy significantly affected the miscarriage rate. Neonatal outcomes, including newborn sex, gestational age, preterm birth, birth weight and low birth weight in the D5 and D6 TE biopsies were not significantly different. Both blastocyst grade and the day of TE biopsy must be considered at the same time when performing preimplantation genetic testing–frozen embryo transfer.

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