Evolution of In Vitro Cowʼs Milk Protein–specific Inflammatory and Regulatory Cytokine Responses in Preterm Infants With Necrotising Enterocolitis

2013 ◽  
Vol 56 (1) ◽  
pp. 5-11 ◽  
Author(s):  
Adel E. Abdelhamid ◽  
Shu-Ling Chuang ◽  
Peter Hayes ◽  
John M.E. Fell
Keyword(s):  
Preterm Infants ◽  
Milk Protein ◽  
Necrotising Enterocolitis ◽  
Cytokine Responses ◽  
Regulatory Cytokine

Impaired Cytokine Responses to Live Staphylococcus epidermidis in Preterm Infants Precede Gram-positive, Late-onset Sepsis

2020 ◽  
Author(s):  
Tobias Strunk ◽  
Julie Hibbert ◽  
Dorota Doherty ◽  
Elizabeth Nathan ◽  
Karen Simmer ◽  
...  
Keyword(s):  
Preterm Infants ◽  
Staphylococcus Epidermidis ◽  
Late Onset ◽  
Gram Positive ◽  
Cytokine Responses ◽  
Inflammatory Protein ◽  
Late Onset Sepsis ◽  
Immunological Mechanisms ◽  
Factor Α

Abstract Background Late-onset sepsis (LOS) with Staphylococcus epidermidis is common in preterm infants, but the immunological mechanisms underlying heightened susceptibility are poorly understood. Our aim is to characterize the ontogeny of cytokine responses to live S. epidermidis in preterm infants with and without subsequent Gram-positive LOS. Methods We conducted a prospective, observational cohort study of preterm infants (<30 weeks gestational age [GA]) with blood sampling on Days 1, 7, 14, 21, and 28 of life. Cytokine responses in peripheral whole blood stimulated with live S. epidermidis were analyzed by 11-plex immunoassay. Results Of 129 infants (mean GA, 26.2 weeks; mean birth weight, 887g), 23 (17.8%) had confirmed LOS with Gram-positive organisms and 15 (11.6%) had clinical sepsis, with median onsets at 13 and 15 days, respectively. Blood cytokine responses to an in vitro S. epidermidis challenge were similar between infected and uninfected infants on Day 1, but diverged thereafter. Infants with subsequent LOS displayed broadly reduced S. epidermidis–induced responses from Day 7 onwards, compared to those who did not develop LOS. This pattern was observed with chemokines (interleukin [IL]-8, monocyte chemotactic protein–1, and macrophage inflammatory protein–1α), pro-inflammatory cytokines (IL-1, IL-6, and tumor necrosis factor–α) and the regulatory cytokine IL-10. Conclusions Cytokine responses to a live S. epidermidis challenge are impaired in infants with LOS and precede the onset of clinical illness. Quantifying pathogen-specific cytokine responses at Day 7 may identify those high-risk preterm infants at the greatest risk of LOS, and prospective replication is warranted.

scholarly journals 52 In Vitro Evidence of Cow's Milk Protein Sensitisation in Neonatal Necrotising Enterocolitis

2004 ◽  
Vol 56 (3) ◽  
pp. 473-473
Author(s):  
S L Chuang ◽  
P Hayes ◽  
E Ogundipe ◽  
N Modi ◽  
M Haddad ◽  
...  
Keyword(s):  
Milk Protein ◽  
Necrotising Enterocolitis ◽  
Cow’S Milk ◽  
Cow's Milk ◽  
Neonatal Necrotising Enterocolitis ◽  
Cow’S Milk Protein

In vitro dynamic gastric digestion of soya protein/milk protein blended beverages: influence of protein composition and co-processing

2021 ◽  
Vol 12 (6) ◽  
pp. 2605-2616
Author(s):  
Teresa Francis Wegrzyn ◽  
Alejandra Acevedo-Fani ◽  
Simon M. Loveday ◽  
Harjinder Singh
Keyword(s):  
Whey Protein ◽  
Milk Protein ◽  
Protein Composition ◽  
Soya Protein ◽  
Gastric Digestion ◽  
Heat Treated ◽  
Protein Milk

The gastric digestion behaviours of blended protein beverages containing different ratios of casein, whey protein and soya protein that were heat-treated at 60 °C or 80 °C were investigated using an in vitro dynamic human gastric simulator.

scholarly journals Human milk feeding and cognitive outcome in preterm infants: the role of infection and NEC reduction

2021 ◽  
Author(s):  
Winok Lapidaire ◽  
Alan Lucas ◽  
Jonathan D. Clayden ◽  
Chris Clark ◽  
Mary S. Fewtrell
Keyword(s):  
Breast Milk ◽  
Human Milk ◽  
Preterm Infants ◽  
Neonatal Infection ◽  
Necrotising Enterocolitis ◽  
Cognitive Outcome ◽  
Standard Diet ◽  
Risk Of Infection ◽  
Verbal Iq

Abstract Background Breast milk has been associated with lower risk of infection and necrotising enterocolitis (NEC) and improved long-term cognitive outcomes in preterm infants but, if unsupplemented, does not meet the nutritional requirements of preterm infants. Methods Preterm infants were randomised to receive a high nutrient intervention diet: preterm formula (PTF) or the standard diet: term formula (TF) or banked donor breast milk (BBM), either as their sole diet or as supplement to maternal breast milk (MBM). IQ tests were performed at ages 7, 15, 20, and 30 years. Results An increase in MBM and BBM intake was associated with a lower chance of neonatal infection/NEC. Neonatal infection/NEC was associated with lower Full Scale IQ (FSIQ) and Performance IQ (PIQ) score at ages 7 and 30 years. The relationship between higher intake of MBM and PIQ at age 7 years was partly mediated by neonatal infection/NEC. The intervention diet was associated with higher Verbal IQ (VIQ) scores compared to the standard diet. There was no evidence that these effects changed from childhood through to adulthood. Conclusions Neonatal diet is an important modifiable factor that can affect long-term cognitive outcome through a ‘human milk’ factor, protecting against infection/NEC, and a ‘nutrient content’ factor. Impact This is the first study to demonstrate the effects of neonatal infection/necrotising enterocolitis (NEC) on IQ in the same cohort in childhood and adulthood. Diet can be a key factor in long-term cognitive outcome in people born preterm by preventing neonatal infection/NEC and providing adequate nutrients. Human milk, whether MBM or BBM, is associated with a reduced risk of infection/NEC. A higher nutrient diet is associated with better cognitive outcome in childhood. Performance IQ is particularly vulnerable to the effects of infection/NEC and verbal IQ to the quantity of (macro)nutrients in the diet.

scholarly journals Association Between In Vitro Bleeding Time and Bleeding in Preterm Infants With Thrombocytopenia

2019 ◽  
Vol 173 (4) ◽  
pp. 393 ◽  
Author(s):  
Emoke Deschmann ◽  
Matthew A. Saxonhouse ◽  
Henry A. Feldman ◽  
Mikael Norman ◽  
Maria Barbian ◽  
...  
Keyword(s):  
Preterm Infants ◽  
Bleeding Time

scholarly journals Interaction of DNA-binding proteins with a milk protein gene promoter in vitro: identification of a mammary gland-specific factor

1991 ◽  
Vol 19 (23) ◽  
pp. 6603-6610 ◽  
Author(s):  
Christine J. Waston ◽  
Katrina E. Gordon ◽  
Morag Robertson ◽  
A. John Clark
Keyword(s):  
Mammary Gland ◽  
Dna Binding ◽  
Milk Protein ◽  
Binding Proteins ◽  
Protein Gene ◽  
Gene Promoter ◽  
Dna Binding Proteins ◽  
Specific Factor ◽  
Milk Protein Gene
Sign in / Sign up

Export Citation Format

Share Document