The severity of brain damage determines bone marrow stromal cell therapy efficacy in a traumatic brain injury model

2012 ◽  
Vol 72 (5) ◽  
pp. 1203-1212 ◽  
Author(s):  
Celia Bonilla ◽  
Mercedes Zurita ◽  
Laura Otero ◽  
Concepción Aguayo ◽  
Miguel A. Rico ◽  
...  
Keyword(s):  
Traumatic Brain Injury ◽  
Bone Marrow ◽  
Brain Injury ◽  
Cell Therapy ◽  
Stromal Cell ◽  
Bone Marrow Stromal Cell ◽  
Marrow Stromal Cell ◽  
Therapy Efficacy ◽  
Traumatic Brain Injury Model ◽  
Injury Model

Long-term recovery after bone marrow stromal cell treatment of traumatic brain injury in rats

2006 ◽  
Vol 104 (2) ◽  
pp. 272-277 ◽  
Author(s):  
Asim Mahmood ◽  
Dunyue Lu ◽  
Changsheng Qu ◽  
Anton Goussev ◽  
Michael Chopp
Keyword(s):  
Traumatic Brain Injury ◽  
Bone Marrow ◽  
Brain Injury ◽  
Functional Outcome ◽  
Stromal Cell ◽  
Bone Marrow Stromal Cell ◽  
Male Rats ◽  
Marrow Stromal Cell ◽  
Injured Brain ◽  
Female Wistar Rats

Object This study was designed to follow the effects of bone marrow stromal cell (BMSC) administration in rats after traumatic brain injury (TBI) for a 3-month period. Methods Forty adult female Wistar rats were injured by a controlled cortical impact and, 1 week later, were injected intravenously with one of three different doses of BMSCs (2 × 106, 4 × 106, or 8 × 106 cells per animal) obtained in male rats. Control rats received phosphate-buffered saline (PBS). Neurological function in these rats was studied using a neurological severity scale (NSS). The rats were killed 3 months after injury, and immunohistochemical stains were applied to brain samples to study the distribution of the BMSCs. Additional brain samples were analyzed by quantitative enzyme-linked immunosorbent assays to measure the expression of the growth factors brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF). Three months after injury, BMSCs were present in the injured brain and their number was significantly greater in animals that received 4 × 106 or 8 × 106 BMSCs than in animals that received 2 × 106 BMSCs. The cells were primarily distributed around the lesion boundary zone. Functional outcome was significantly better in rats that received 4 × 106 or 8 × 106 BMSCs, compared with control animals, although no improvement was seen in animals that received 2 × 106 BMSCs. All doses of BMSCs significantly increased the expression of BDNF but not that of NGF; however, this increase was significantly larger in animals that received 4 × 106 or 8 × 106 BMSCs than in controls or animals that received 2 × 106 BMSCs. Conclusions In summary, when injected in rats after TBI, BMSCs are present in the brain 3 months later and significantly improve functional outcome.

scholarly journals MRI evaluation of axonal reorganization after bone marrow stromal cell treatment of traumatic brain injury

2011 ◽  
Vol 24 (9) ◽  
pp. 1119-1128 ◽  
Author(s):  
Quan Jiang ◽  
Changsheng Qu ◽  
Michael Chopp ◽  
Guang Liang Ding ◽  
Siamak P. Nejad- Davarani ◽  
...  
Keyword(s):  
Traumatic Brain Injury ◽  
Bone Marrow ◽  
Brain Injury ◽  
Stromal Cell ◽  
Bone Marrow Stromal Cell ◽  
Marrow Stromal Cell ◽  
Mri Evaluation

Intravenous bone marrow stromal cell therapy reduces apoptosis and promotes endogenous cell proliferation after stroke in female rat

2003 ◽  
Vol 73 (6) ◽  
pp. 778-786 ◽  
Author(s):  
Jieli Chen ◽  
Yi Li ◽  
Mark Katakowski ◽  
Xiaoguang Chen ◽  
Lei Wang ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Cell Proliferation ◽  
Cell Therapy ◽  
Stromal Cell ◽  
Bone Marrow Stromal Cell ◽  
Female Rat ◽  
Marrow Stromal Cell
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