scholarly journals Lack of fidelity revealed in an insect–fungal mutualism after invasion

2013 ◽  
Vol 9 (4) ◽  
pp. 20130342 ◽  
Author(s):  
Amy L. Wooding ◽  
Michael J. Wingfield ◽  
Brett P. Hurley ◽  
Jeffrey R. Garnas ◽  
Peter de Groot ◽  
...  
Keyword(s):  
North America ◽  
Biological Invasions ◽  
Community Change ◽  
Fungal Species ◽  
Biological Mechanisms ◽  
Culture Methods ◽  
Sirex Noctilio ◽  
Sirex Nigricornis ◽  
Obligate Mutualism ◽  
Novel Associations

Symbiont fidelity is an important mechanism in the evolution and stability of mutualisms. Strict fidelity has been assumed for the obligate mutualism between Sirex woodwasps and their mutualistic Amylostereum fungi. This assumption has been challenged in North America where the European woodwasp, Sirex noctilio , and its fungal mutualist, Amylostereum areolatum , have recently been introduced. We investigate the specificity of the mutualism between Sirex and Amylostereum species in Canada, where S. noctilio co-infests Pinus with native Sirex nigricornis and its mutualist, Amylostereum chailletii . Using phylogenetic and culture methods, we show that extensive, reciprocal exchange of fungal species and strains is occurring, with 75.3 per cent of S. nigricornis carrying A. areolatum and 3.5 per cent of S. noctilio carrying A. chailletii . These findings show that the apparent specificity of the mutualism between Sirex spp. and their associated Amylostereum spp. is not the result of specific biological mechanisms that maintain symbiont fidelity. Rather, partner switching may be common when shifting geographical distributions driven by ecological or anthropogenic forces bring host and mutualist pairs into sympatry. Such novel associations have potentially profound consequences for fitness and virulence. Symbiont sharing, if it occurs commonly, may represent an important but overlooked mechanism of community change linked to biological invasions.

Intergenic Spacer Single Nucleotide Polymorphisms for Genotyping Amylostereum areolatum (Russulales: Amylostereacea) Symbionts of Native and Non-native Sirex Species

2020 ◽  
Vol 113 (4) ◽  
pp. 280-287 ◽  
Author(s):  
Rabiu O Olatinwo ◽  
Timothy D Schowalter ◽  
Daniel Doucet ◽  
Susan Bowman ◽  
Wood C Johnson ◽  
...  
Keyword(s):  
North America ◽  
Single Nucleotide Polymorphisms ◽  
Genetic Markers ◽  
Native Species ◽  
Intergenic Spacer ◽  
Snp Markers ◽  
Nucleotide Polymorphisms ◽  
Sirex Noctilio ◽  
Single Nucleotide ◽  
Sirex Nigricornis

Abstract In North America Amylostereum areolatum (Chaillet ex Fr.) Boidin is a fungal symbiont associated with both the non-native Sirex noctilio Fabricius (Hymenoptera: Siricidae) and less commonly the native Sirex nigricornis Fabricius (Hymenoptera: Siricidae) woodwasps. The relationship between S. noctilio and A. areolatum constitutes a serious threat to pine plantation in the southern hemisphere. Studies have shown evidence of exchange of symbionts between non-native and native Sirex species. Our objectives were 1) to identify and assemble a panel of rDNA intergenic spacer–single nucleotide polymorphisms (IGS-SNPs) for genotyping strains of A. areolatum symbionts associated with Sirex species in North America, and 2) to develop genetic markers for monitoring the spread of specific A. areolatum haplotypes associated with S. noctilio across regions. The IGS-SNPs panel analyzed included haplotypes B1, B2, D1, D2 (from known IGS type B and D), E, and F. Genetic markers and haplotype-specific primers were designed to detect the IGS haplotypes D and E of A. areolatum. We found that haplotype D was absent in A. areolatum from S. nigricornis in Louisiana, while haplotype E was detected in all A. areolatum from S. nigricornis in Canada and Louisiana. Both haplotype D and E were co-detected in approximately 5% of samples from Canada. The IGS-SNP markers detected specific haplotypes accurately. Observing haplotype D in any A. areolatum from the native S. nigricornis likely indicates the presence of the potentially harmful S. noctilo-A. areolatum complex. The work highlights how IGS-SNPs can help in early detection without direct occurrence/observations of the non-native species of concern.

scholarly journals Inter- and Intra-Continental Genetic Variation in the Generalist Conifer Wood Saprobic Fungus Phlebiopsis gigantea

Forests ◽  
2021 ◽  
Vol 12 (6) ◽  
pp. 751
Author(s):  
Francesco Dovana ◽  
Paolo Gonthier ◽  
Matteo Garbelotto
Keyword(s):  
North America ◽  
Genetic Differentiation ◽  
Dna Sequences ◽  
North American ◽  
Fungal Species ◽  
Coniferous Forests ◽  
Native Forest ◽  
Western North America ◽  
Phlebiopsis Gigantea ◽  
Conifer Wood

Phlebiopsis gigantea (Fr.) Jülich is a well-known generalist conifer wood saprobe and a biocontrol fungus used in several world countries to prevent stump infection by tree pathogenic Heterobasidion fungal species. Previous studies have reported the presence of regional and continental genetic differentiation in host-specific fungi, but the presence of such differentiation for generalist wood saprobes such as P. gigantea has not been often studied or demonstrated. Additionally, little information exists on the distribution of this fungus in western North America. The main purposes of this study were: (I) to assess the presence of P. gigantea in California, (II) to explore the genetic variability of P. gigantea at the intra and inter-continental levels and (III) to analyze the phylogeographic relationships between American and European populations. Seven loci (nrITS, ML5–ML6, ATP6, RPB1, RPB2, GPD and TEF1-α) from 26 isolates of P. gigantea from coniferous forests in diverse geographic distribution and from different hosts were analyzed in this study together with 45 GenBank sequences. One hundred seventy-four new sequences were generated using either universal or specific primers designed in this study. The mitochondrial ML5–ML6 DNA and ATP6 regions were highly conserved and did not show differences between any of the isolates. Conversely, DNA sequences from the ITS, RPB1, RPB2, GPD and TEF1-α loci were variable among samples. Maximum likelihood analysis of GPD and TEF1-α strongly supported the presences of two different subgroups within the species but without congruence or geographic partition, suggesting the presence of retained ancestral polymorphisms. RPB1 and RPB2 sequences separated European isolates from American ones, while the GPD locus separated western North American samples from eastern North American ones. This study reports the presence of P. gigantea in California for the first time using DNA-based confirmation and identifies two older genetically distinct subspecific groups, as well as three genetically differentiated lineages within the species: one from Europe, one from eastern North America and one from California, with the latter presumably including individuals from the rest of western North America. The genetic differentiation identified here among P. gigantea individuals from coniferous forests from different world regions indicates that European isolates of this fungus should not be used in North America (or vice versa), and, likewise, commercially available eastern North American P. gigantea isolates should not be used in western North America forests. The reported lack of host specificity of P. gigantea was documented by the field survey and further reinforces the need to only use local isolates of this biocontrol fungus, given that genetically distinct exotic genotypes of a broad generalist microbe may easily spread and permanently alter the microbial biodiversity of native forest ecosystems.

scholarly journals Creativity, Community, Change: Functions of and motives for singing niggunim

1970 ◽  
Vol 27 ◽  
pp. 284-305
Author(s):  
Ruth Illman
Keyword(s):  
North America ◽  
Community Change ◽  
Religious Communities ◽  
Jewish Communities ◽  
Musical Practices ◽  
Mystical Traditions ◽  
Depth Interviews

Jewish musical practices stemming from Kabbalah and Hasidic mystical traditions are currently the object of growing attention among a variety of different Jewish communities in Europe and North America, as well as in non-Jewish spiritual circles. This article focuses on contemporary practices of niggunim – the (mostly) wordless melodies with roots in Hasidic Jewish traditions, sung, chanted and sometimes danced in preparation for, or as a form of, ardent prayer. The practice is seen as an example of the expressive, engaging, emotional and embodied forms of prayer that currently attract many Jews of different institutional attachments. As niggunim travel into new contexts, they are reframed and reconsidered in order to meet the needs and expectations of contemporary religious communities, characterised by a liberal and egalitarian, global and transformative religiosity. The article seeks to explore the different functions niggunim are put to today and the motives which drive different people to engage in the practice. The analysis is based on ethno-graphic material in the form of in-depth interviews conducted among progressive Jews in the London area. As a conclusion, the article suggests an approach to contemporary niggunim practices that incorporates perspectives from both literature and ethnography in order to deepen the understanding of the motives for and functions of singing niggunim today.

scholarly journals Paleogene community change among terrestrial vertebrates of the Western Hemisphere

1992 ◽  
Vol 6 ◽  
pp. 282-282
Author(s):  
Richard K. Stucky
Keyword(s):  
North America ◽  
South America ◽  
Community Change ◽  
Taxonomic Composition ◽  
Western Hemisphere ◽  
Dental Morphology ◽  
Predator Prey ◽  
Terrestrial Vertebrates ◽  
Morphological Adaptations ◽  
Dinosaur Extinction

Paleogene vertebrate communities in North and South America show dramatic changes in taxonomic composition and ecological organization. Worldwide, mammals diversified substantially following dinosaur extinction (Fig. 1). Most families of living vertebrates appear by the end of the Paleogene. In North America, placental omnivores, herbivores and carnivores dominate mammalian communities, but in South America marsupial carnivores and omnivores and placental herbivores dominate them. Immigration from Asia and Europe to North America of taxa from several placental orders (Perissodactyla, Primates, Artiodactyla, Rodentia, Carnivora, Mesonychia, Creodonta) occurred periodically during the Paleogene. South America, however, was completely isolated from the Paleocene to the Oligocene when Rodentia and perhaps Primates first appear. Despite the independent evolutionary histories of these continents, their constituent species show remarkable convergences in morphological adaptations including body size distributions, dental morphology, and other features. Low resolution chronostratigraphic data for the Paleogene of South America precludes correlation with North American faunas. In North America, patterns of diversification and extinction appear to be related to climatic events. Morphological convergences appear to be related to climate and concomitant habitat change, but may also be a function of coevolution via predator-prey interactions and diffuse competition among guild members.

Investigating the effects of symbiotic fungi on the flight behaviour ofSirex noctilio(Hymenoptera: Siricidae)

2016 ◽  
Vol 148 (5) ◽  
pp. 543-551 ◽  
Author(s):  
Mark A. Sarvary ◽  
Ann E. Hajek ◽  
Katalin Böröczky ◽  
Robert A. Raguso ◽  
Miriam F. Cooperband
Keyword(s):  
Fungal Species ◽  
Flight Tunnel ◽  
White Rot ◽  
White Rot Fungus ◽  
Sirex Noctilio ◽  
Flight Behaviour ◽  
Native North American ◽  
Complete Chemical Analysis ◽  
Volatile Profiles ◽  
Symbiotic Fungi

AbstractThe invasive woodwaspSirex noctilioFabricius (Hymenoptera: Siricidae) is obligately associated with the symbiotic white rot fungusAmylostereum areolatum(Chaillet ex Fries) Boidin (Basidiomycota: Amylosteraceae), and shows positive chemotaxis to volatiles emitted by this symbiont. After introduction to North America,S. noctiliowas collected carrying another fungus speciesAmylostereum chailletii(Persoon) Boidin, used symbiotically by native North AmericanSirexLinnaeus. We conducted flight behaviour studies in a walk-in flight tunnel to evaluate specificity of the attraction of mated and unmatedS. noctilioto its primary symbiont,A. areolatum, versus the alternative symbiont,A. chailletii. Fewer unmated than matedS. noctiliofemales responded to either of the fungi. Unmated females showed no landing preference but matedS. noctiliofemales were attracted toA. areolatumalthough avoidance ofA. chailletiiwas not complete. Chemical analysis demonstrated major differences in the volatile profiles of the two fungal species. Sesquiterpenes dominated theA. areolatumsamples, whereas only two aromatic volatiles were consistently present in the nativeA. chailletii.

Interactions between the fungal symbiont of Sirex noctilio (Hymenoptera: Siricidae) and two bark beetle-vectored fungi

2011 ◽  
Vol 143 (3) ◽  
pp. 224-235 ◽  
Author(s):  
Kathleen Ryan ◽  
Jean-Marc Moncalvo ◽  
Peter de Groot ◽  
Sandy M. Smith
Keyword(s):  
Larval Development ◽  
Bark Beetles ◽  
Essential Role ◽  
Geographic Area ◽  
Fungal Species ◽  
Competitive Interactions ◽  
Effect Of Temperature ◽  
Fungal Symbiont ◽  
Sirex Noctilio ◽  
Individual Trees

AbstractThe woodwasp Sirex noctilio F. is invading North American forests, where it will interact with a large guild of pine-inhabiting beetles and their associated fungi. The woodwasp's obligate fungal symbiont, Amylostereum areolatum (Fries) Boidin (Stereaceae), plays an essential role in the wasp's larval development but is expected to be a poor competitor in the presence of fungi vectored by co-occurring insects. We examined the outcomes of competitive interactions between A. areolatum and two fungal species vectored by bark beetles, Leptographium wingfieldii Morelet (Ophiostomataceae) and Ophiostoma minus (Hedgcock) H. and P. Sydow (Ophiostomataceae), and the effect of temperature and substrate on these interactions. Beetle-associated fungi were usually able to capture more uncolonized resource than A. areolatum regardless of substrate or temperature. Amylostereum areolatum was able to colonize relatively more space in some cases but could not gain substrate already colonized by the ophiostomatoid competitor. These findings suggest that competitive interactions between beetle-vectored fungal species and A. areolatum could influence the reproductive fitness and distribution of S. noctilio within individual trees and also across a wide geographic area.

Sirex species (Hymenoptera: Siricidae) and their parasitoids in Pinus sylvestris in eastern North America

2009 ◽  
Vol 141 (2) ◽  
pp. 153-157 ◽  
Author(s):  
Stefan J. Long ◽  
David W. Williams ◽  
Anne E. Hajek
Keyword(s):  
New York ◽  
Pinus Sylvestris ◽  
New York State ◽  
Eastern North America ◽  
Parasitoid Species ◽  
Sirex Noctilio ◽  
York State ◽  
Sirex Nigricornis ◽  
Percent Parasitism ◽  
Central New York

AbstractSiricids and their parasitoids were reared from Scots pine (Pinus sylvestris L. (Pinaceae)) trees infested by Sirex noctilio F. in central New York State. Sirex noctilio accounted for 94.3% of the siricid specimens emerging, totaling 1313 specimens from six trees, with a maximum of 495 from one tree. Of the individuals emerging per tree, 20.6 ± 5.2% were female. Two native siricids, Sirex nigricornis F. and S. edwardsii Brullè, also emerged from trees but in low numbers. Three hymenopteran parasitoid species that attack siricids emerged, totaling 21.8 ± 6.4% parasitism per tree. Ibalia leucospoides ensiger Norton (Ibaliidae) was by far the most abundant parasitoid, at 20.5 ± 6.3% parasitism per tree. The percentage of female S. noctilio emerging was positively correlated with wood diameter, whereas percent parasitism by I. l. ensiger was negatively correlated with wood diameter.

scholarly journals Two decades of data reveal that Biological Invasions needs to increase participation beyond North America, Europe, and Australasia

2021 ◽  
Author(s):  
Martin A. Nuñez ◽  
Mariana C. Chiuffo ◽  
Hanno Seebens ◽  
Sara Kuebbing ◽  
Matthew A. McCary ◽  
...  
Keyword(s):  
North America ◽  
Biological Invasions
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