Extraordinarily rapid proliferation of cultured muscle satellite cells from migratory birds

Migratory birds experience bouts of muscle growth and depletion as they prepare for, and undertake prolonged flight. Our studies of migratory bird muscle physiology in vitro led to the discovery that sanderling ( Calidris alba ) muscle satellite cells proliferate more rapidly than other normal cell lines. Here we determined the proliferation rate of muscle satellite cells isolated from five migratory species (sanderling; ruff, Calidris pugnax ; western sandpiper, Calidris mauri ; yellow-rumped warbler, Setophaga coronata ; Swainson's thrush, Catharus ustulatus ) from two families (shorebirds and songbirds) and with different migratory strategies. Ruff and sanderling satellite cells exhibited rapid proliferation, with population doubling times of 9.3 ± 1.3 and 11.4 ± 2 h, whereas the remaining species' cell doubling times were greater than or equal to 24 h. The results indicate that the rapid proliferation of satellite cells is not associated with total migration distance but may be related to flight bout duration and interact with lifespan.

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miR-194-5p negatively regulates the proliferation and differentiation of rabbit skeletal muscle satellite cells

Molecular and Cellular Biochemistry ◽

10.1007/s11010-020-03918-0 ◽

2020 ◽

Author(s):

Yu Shi ◽

Xudong Mao ◽

Mingcheng Cai ◽

Shenqiang Hu ◽

Xiulan Lai ◽

...

Keyword(s):

Skeletal Muscle ◽

Satellite Cells ◽

Mammalian Species ◽

Muscle Growth ◽

Luciferase Reporter ◽

Stem Cell Population ◽

Muscle Satellite Cells ◽

Skeletal Muscle Satellite Cells ◽

Proliferation And Differentiation

Abstract Skeletal muscle satellite cells (SMSCs), also known as a multipotential stem cell population, play a crucial role during muscle growth and regeneration. In recent years, numerous miRNAs have been associated with the proliferation and differentiation of SMSCs in a number of mammalian species; however, the regulatory mechanisms of miR-194-5p in rabbit SMSCs still remain scarce. In this study, miR-194-5p was first observed to be highly expressed in the rabbit leg muscle. Furthermore, both the mimics and inhibitor of miR-194-5p were used to explore its role in the proliferation and differentiation of rabbit SMSCs cultured in vitro. Results from both EdU and CCK8 assays showed that miR-194-5p inhibited the proliferation of SMSCs. Meanwhile, Mef2c was identified as a target gene of miR-194-5p based on the dual-luciferase reporter assay results. In addition, upregulation of miR-194-5p decreased the expression levels of Mef2c and MyoG during rabbit SMSCs differentiation on Days 3 and 7 of in vitro culture. Taken together, these data demonstrated that miR-194-5p negatively regulates the proliferation and differentiation of rabbit SMSCs by targeting Mef2c.

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HDAC4 Regulates the Proliferation, Differentiation and Apoptosis of Chicken Skeletal Muscle Satellite Cells

Animals ◽

10.3390/ani10010084 ◽

2020 ◽

Vol 10 (1) ◽

pp. 84 ◽

Cited By ~ 3

Author(s):

Jing Zhao ◽

Xiaoxu Shen ◽

Xinao Cao ◽

Haorong He ◽

Shunshun Han ◽

...

Keyword(s):

Skeletal Muscle ◽

Satellite Cells ◽

Muscle Development ◽

Critical Role ◽

Muscle Growth ◽

Positive Role ◽

Muscle Satellite Cells ◽

Skeletal Muscle Satellite Cells ◽

Chicken Skeletal Muscle

The development of skeletal muscle satellite cells (SMSCs) is a complex process that could be regulated by many genes. Previous studies have shown that Histone Deacetylase 4 (HDAC4) plays a critical role in cell proliferation, differentiation, and apoptosis in mouse. However, the function of HDAC4 in chicken muscle development is still unknown. Given that chicken is a very important meat-producing animal that is also an ideal model to study skeletal muscle development, we explored the functions of HDAC4 in chicken SMSCs after the interference of HDAC4. The results showed that HDAC4 was enriched in embryonic skeletal muscle, and it was highly expressed in embryonic muscle than in postnatal muscles. Meanwhile, knockdown of HDAC4 could significantly inhibit the proliferation and differentiation of chicken SMSCs but had no effect on the apoptosis of SMSCs as observed in a series of experiment conducted in vitro. These results indicated that HDAC4 might play a positive role in chicken skeletal muscle growth and development.

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Isolation, Culture and Biological Characteristics of Tibetan Mastiff (Canis lupus Familiaris) Muscle Satellite Cells

Journal of Biomaterials and Tissue Engineering ◽

10.1166/jbt.2019.2030 ◽

2019 ◽

Vol 9 (5) ◽

pp. 573-582

Author(s):

Tengfei Lu ◽

Xishuai Wang ◽

Yanjie Zheng ◽

Hebao Wen ◽

Hongda Ji ◽

...

Keyword(s):

Satellite Cells ◽

Muscle Growth ◽

Canis Lupus Familiaris ◽

Multipotent Stem Cells ◽

Muscle Satellite Cells ◽

Long Time ◽

Solid Foundation ◽

S Curve ◽

Technical Basis

With the ability of self-renewal and multipotenntial differentiation, muscle satellite cells (MSCs) are recognized as a population of tissue-specific progenitors which play an important role in the growth, repair and regeneration of muscles. Satellite cells have been isolated for a very long time, but the techniques of isolation are still to be improved. In this study, MSCs were obtained from aborted Tibetan mastiff fetuses (about 60 days) under sterile conditions. Primary MSCs were sub-cultured to passage 25 in vitro. The gene of C-met, MyoD1, Desmin and Pax7 were detected by RT-polymerase chain reaction and immunofluorescence assays. The result showed that they were positive in MSCs of Tibetan mastiff. The growth of different passages cells typically appeared in S curve. Furthermore, myogenic, osteogenic and adipogenic differenciation of MSCs were successfully induced. The results denoted that the MSCs obtained from Tibetan Mastiffs exhibited the characteristics of multipotent stem cells. Therefore, this work provided a theoretical and technical basis for Tibetan mastiff, even its genetic resources preservation and laid a solid foundation for studying the mechanism of skeletal muscle growth and development in future.

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Sex, fiber-type, and age dependent in vitro proliferation of mouse muscle satellite cells

Journal of Cellular Biochemistry ◽

10.1002/jcb.23197 ◽

2011 ◽

Vol 112 (10) ◽

pp. 2825-2836 ◽

Cited By ~ 23

Author(s):

Raquel Manzano ◽

Janne M. Toivonen ◽

Ana C. Calvo ◽

Francisco J. Miana-Mena ◽

Pilar Zaragoza ◽

...

Keyword(s):

Satellite Cells ◽

Fiber Type ◽

In Vitro Proliferation ◽

Mouse Muscle ◽

Muscle Satellite Cells ◽

Age Dependent

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Sensitivity of muscle satellite cells to pollutants: an in vitro and in vivo comparative approach

Aquatic Toxicology ◽

10.1016/s0166-445x(01)00170-9 ◽

2001 ◽

Vol 53 (3-4) ◽

pp. 247-263 ◽

Cited By ~ 13

Author(s):

B Fauconneau ◽

G Paboeuf

Keyword(s):

Satellite Cells ◽

Comparative Approach ◽

Muscle Satellite Cells

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Mechanical stretch-induced activation of skeletal muscle satellite cells is dependent on nitric oxide production in vitro

Animal Science Journal ◽

10.1046/j.1344-3941.2002.00033.x ◽

2002 ◽

Vol 73 (3) ◽

pp. 235-239 ◽

Cited By ~ 14

Author(s):

Ryuichi TATSUMI ◽

Akihito HATTORI ◽

Ronald E. ALLEN ◽

Yoshihide IKEUCHI ◽

Tatsumi ITO

Keyword(s):

Nitric Oxide ◽

Skeletal Muscle ◽

Satellite Cells ◽

Mechanical Stretch ◽

Nitric Oxide Production ◽

Muscle Satellite Cells ◽

Skeletal Muscle Satellite Cells ◽

Oxide Production

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MiR-27b Promotes Muscle Development by Inhibiting MDFI Expression

Cellular Physiology and Biochemistry ◽

10.1159/000489595 ◽

2018 ◽

Vol 46 (6) ◽

pp. 2271-2283 ◽

Cited By ~ 12

Author(s):

Lianjie Hou ◽

Jian Xu ◽

Yiren Jiao ◽

Huaqin Li ◽

Zhicheng Pan ◽

...

Keyword(s):

Skeletal Muscle ◽

Western Blot ◽

Satellite Cells ◽

Muscle Regeneration ◽

Muscle Injury ◽

Target Gene ◽

Muscle Development ◽

Muscle Satellite Cells ◽

Qrt Pcr

Background/Aims: Skeletal muscle plays an essential role in the body movement. However, injuries to the skeletal muscle are common. Lifelong maintenance of skeletal muscle function largely depends on preserving the regenerative capacity of muscle. Muscle satellite cells proliferation, differentiation, and myoblast fusion play an important role in muscle regeneration after injury. Therefore, understanding of the mechanisms associated with muscle development during muscle regeneration is essential for devising the alternative treatments for muscle injury in the future. Methods: Edu staining, qRT-PCR and western blot were used to evaluate the miR-27b effects on pig muscle satellite cells (PSCs) proliferation and differentiation in vitro. Then, we used bioinformatics analysis and dual-luciferase reporter assay to predict and confirm the miR-27b target gene. Finally, we elucidate the target gene function on muscle development in vitro and in vivo through Edu staining, qRT-PCR, western blot, H&E staining and morphological observation. Result: miR-27b inhibits PSCs proliferation and promotes PSCs differentiation. And the miR-27b target gene, MDFI, promotes PSCs proliferation and inhibits PSCs differentiation in vitro. Furthermore, interfering MDFI expression promotes mice muscle regeneration after injury. Conclusion: our results conclude that miR-27b promotes PSCs myogenesis by targeting MDFI. These results expand our understanding of muscle development mechanism in which miRNAs and genes work collaboratively in regulating skeletal muscle development. Furthermore, this finding has implications for obtaining the alternative treatments for patients with the muscle injury.

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