scholarly journals A lateral flow immunochromatographic strip test for rapid detection of hexoestrol in fish samples

2018 ◽  
Vol 5 (8) ◽  
pp. 180504 ◽  
Author(s):  
Xingdong Yang ◽  
Zhongke Sun ◽  
Fengshou Tian ◽  
Guochao Jia ◽  
Jifei Yang ◽  
...  
Keyword(s):  
High Performance ◽  
Limit Of Detection ◽  
Lateral Flow ◽  
Control Line ◽  
Immunochromatographic Strip ◽  
Coefficients Of Variation ◽  
Fish Samples ◽  
Gold Labelling ◽  
Immunochromatographic Strip Test ◽  
Strip Test

A lateral flow immunochromatographic strip test was developed for rapid and sensitive on-site detection of hexoestrol (HES) residues in fish samples with colloidal gold labelling of the anti-HES monoclonal antibody. The strip is composed of a sample pad, a conjugate reagent pad, an absorbent pad and a test membrane containing a control line and a test line. The sensitivity (half inhibitory concentration, IC 50 ) of the strip in the detection of fish extract samples was confirmed to be 1.86 µg kg −1 , and the limit of detection value was 0.62 µg kg −1 . For intra-assay and inter-assay reproducibility, recoveries of HES-spiked samples ranged from 86.3% to 92.3% and 85.8% to 93.4%, coefficients of variation were 2.91–4.64% and 4.24–5.17%, respectively. High-performance liquid chromatography was employed to confirm the performance of the strip. The strip test takes less than 10 min, and thus provides a repaid method for on-site detection of HES residues.

scholarly journals Immunochromatographic Strip Test for Rapid Detection of Nevirapine in Plasma Samples from Human Immunodeficiency Virus-Infected Patients

2007 ◽  
Vol 51 (9) ◽  
pp. 3361-3363 ◽  
Author(s):  
Tim R. Cressey ◽  
Sawitree Nangola ◽  
Yardpiroon Tawon ◽  
Mookda Pattarawarapan ◽  
Marc Lallemant ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Limit Of Detection ◽  
Drug Level ◽  
Plasma Samples ◽  
Immunochromatographic Strip ◽  
Resource Limited ◽  
Qualitative Detection ◽  
Immunodeficiency Virus ◽  
Immunochromatographic Strip Test ◽  
Strip Test

ABSTRACT We report a novel one-step immunochromatographic strip test for the rapid, qualitative detection of nevirapine in plasma samples from human immunodeficiency virus-infected patients. The sensitivity was 100% (95% confidence interval [95% CI], 97.8 to 100%), and the specificity was 99.5% (95% CI, 97.2 to 99.9%). The limit of detection was 25 ng/ml. Immunochromatographic strip tests are simple, rapid, and cheap assays that could greatly facilitate drug level monitoring in resource-limited settings.

Developmental Validation of RSID™-Semen: A Lateral Flow Immunochromatographic Strip Test for the Forensic Detection of Human Semen*

2011 ◽  
Vol 57 (2) ◽  
pp. 489-499 ◽  
Author(s):  
Jennifer Old ◽  
Brett A. Schweers ◽  
Pravat W. Boonlayangoor ◽  
Brian Fischer ◽  
Kevin W. P. Miller ◽  
...  
Keyword(s):  
Lateral Flow ◽  
Human Semen ◽  
Immunochromatographic Strip ◽  
Developmental Validation ◽  
Immunochromatographic Strip Test ◽  
Strip Test

Stability of control materials for α-thalassemia immunochromatographic strip test

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Paweena Yanpanya ◽  
Chedtapak Ruengdit ◽  
Panida Pongpunyayuen ◽  
Sakorn Pornprasert
Keyword(s):  
Immunochromatographic Strip ◽  
Immunochromatographic Strip Test ◽  
Strip Test

Development of a rapid, immunochromatographic strip test for serum asialo α1-acid glycoprotein in patients with hepatic disease

2006 ◽  
Vol 308 (1-2) ◽  
pp. 116-123 ◽  
Author(s):  
Eun Young Lee ◽  
Ji Hyun Kang ◽  
Kyoung A. Kim ◽  
Tai Wha Chung ◽  
Hee Jung Kim ◽  
...  
Keyword(s):  
Hepatic Disease ◽  
Immunochromatographic Strip ◽  
Acid Glycoprotein ◽  
Immunochromatographic Strip Test ◽  
Strip Test

Development of an Immunochromatographic Strip Test for Rapid Detection of Melamine in Raw Milk, Milk Products and Animal Feed

2011 ◽  
Vol 59 (11) ◽  
pp. 6064-6070 ◽  
Author(s):  
Xiangmei Li ◽  
Pengjie Luo ◽  
Shusheng Tang ◽  
Ross C. Beier ◽  
Xiaoping Wu ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Animal Feed ◽  
Raw Milk ◽  
Immunochromatographic Strip ◽  
Milk Products ◽  
Immunochromatographic Strip Test ◽  
Strip Test

scholarly journals Rapid immunoassays for detection of anabolic nortestosterone in dietary supplements

2013 ◽  
Vol 31 (No. 5) ◽  
pp. 514-519 ◽  
Author(s):  
B. Holubová ◽  
S. Göselová ◽  
L. Ševčíková ◽  
M. Vlach ◽  
M. Blažková ◽  
...  
Keyword(s):  
Dietary Supplements ◽  
Rapid Detection ◽  
Visual Detection ◽  
Limit Of Detection ◽  
Polyclonal Antibodies ◽  
Cross Reactivity ◽  
Rapid Analysis ◽  
Experimental Conditions ◽  
Immunochromatographic Strip ◽  
Strip Test

An enzyme immunoassay (ELISA) and an immunochromatographic strip were designed for a rapid detection of nortestosterone in dietary supplements. Two polyclonal antibodies and two types of nortestosterone-protein coating conjugates were tested to develop the most appropriate method. Under optimal experimental conditions, the most sensitive ELISA achieved the IC<sub>50 </sub>and the limit of detection values of 6.41 and 0.09 ng/ml, respectively. The assay specificity was tested measuring cross-reactivity of several steroids. The interference with the assay was negligible (&lt; 0.1%), except for cross-reactivity with another frequently abused steroid testosterone (23%). The optimised gold particle-based immunochromatographic strip provided in semi-quantitative test a visual detection limit of 1&nbsp;ng/ml. None of these methods showed the interference using a filtrate of the suspension of non-contaminated sample. After the validation for particular matrices, the ELISA and the strip test could be useful tools for a rapid analysis of nortestosterone in crude extracts of dietary supplements.

Sign in / Sign up

Export Citation Format

Share Document