Developmental Validation of RSID™-Saliva: A Lateral Flow Immunochromatographic Strip Test for the Forensic Detection of Saliva

2009 ◽  
Vol 54 (4) ◽  
pp. 866-873 ◽  
Author(s):  
Jennifer B. Old ◽  
Brett A. Schweers ◽  
Pravat W. Boonlayangoor ◽  
Karl A. Reich
2011 ◽  
Vol 57 (2) ◽  
pp. 489-499 ◽  
Author(s):  
Jennifer Old ◽  
Brett A. Schweers ◽  
Pravat W. Boonlayangoor ◽  
Brian Fischer ◽  
Kevin W. P. Miller ◽  
...  

2018 ◽  
Vol 5 (8) ◽  
pp. 180504 ◽  
Author(s):  
Xingdong Yang ◽  
Zhongke Sun ◽  
Fengshou Tian ◽  
Guochao Jia ◽  
Jifei Yang ◽  
...  

A lateral flow immunochromatographic strip test was developed for rapid and sensitive on-site detection of hexoestrol (HES) residues in fish samples with colloidal gold labelling of the anti-HES monoclonal antibody. The strip is composed of a sample pad, a conjugate reagent pad, an absorbent pad and a test membrane containing a control line and a test line. The sensitivity (half inhibitory concentration, IC 50 ) of the strip in the detection of fish extract samples was confirmed to be 1.86 µg kg −1 , and the limit of detection value was 0.62 µg kg −1 . For intra-assay and inter-assay reproducibility, recoveries of HES-spiked samples ranged from 86.3% to 92.3% and 85.8% to 93.4%, coefficients of variation were 2.91–4.64% and 4.24–5.17%, respectively. High-performance liquid chromatography was employed to confirm the performance of the strip. The strip test takes less than 10 min, and thus provides a repaid method for on-site detection of HES residues.


Author(s):  
Paweena Yanpanya ◽  
Chedtapak Ruengdit ◽  
Panida Pongpunyayuen ◽  
Sakorn Pornprasert

2007 ◽  
Vol 51 (9) ◽  
pp. 3361-3363 ◽  
Author(s):  
Tim R. Cressey ◽  
Sawitree Nangola ◽  
Yardpiroon Tawon ◽  
Mookda Pattarawarapan ◽  
Marc Lallemant ◽  
...  

ABSTRACT We report a novel one-step immunochromatographic strip test for the rapid, qualitative detection of nevirapine in plasma samples from human immunodeficiency virus-infected patients. The sensitivity was 100% (95% confidence interval [95% CI], 97.8 to 100%), and the specificity was 99.5% (95% CI, 97.2 to 99.9%). The limit of detection was 25 ng/ml. Immunochromatographic strip tests are simple, rapid, and cheap assays that could greatly facilitate drug level monitoring in resource-limited settings.


2006 ◽  
Vol 308 (1-2) ◽  
pp. 116-123 ◽  
Author(s):  
Eun Young Lee ◽  
Ji Hyun Kang ◽  
Kyoung A. Kim ◽  
Tai Wha Chung ◽  
Hee Jung Kim ◽  
...  

2011 ◽  
Vol 59 (11) ◽  
pp. 6064-6070 ◽  
Author(s):  
Xiangmei Li ◽  
Pengjie Luo ◽  
Shusheng Tang ◽  
Ross C. Beier ◽  
Xiaoping Wu ◽  
...  

2018 ◽  
Vol 19 (10) ◽  
pp. 3013 ◽  
Author(s):  
Trinh Thuy Tien ◽  
Hyun Park ◽  
Hien Tuong ◽  
Seung-Taek Yu ◽  
Du-Young Choi ◽  
...  

Human respiratory syncytial virus (RSV) is one of the most common viruses infecting the respiratory tracts of infants. The rapid and sensitive detection of RSV is important to minimize the incidence of infection. In this study, novel monoclonal antibodies (mAbs; B11A5 and E8A11) against RSV nucleoprotein (NP) were developed and applied to develop a rapid fluorescent immunochromatographic strip test (FICT), employing europium nanoparticles as the fluorescent material. For the FICT, the limits of detection of the antigen and virus were 1.25 µg/mL and 4.23 × 106 TCID50/mL, respectively, corresponding to 4.75 × 106 ± 5.8 ×105 (mean ± SD) RNA copy numbers per reaction mixture for RSV NP. A clinical study revealed a sensitivity of 90% (18/20) and specificity of 98.18% (108/110) for RSV detection when comparing the performance to that of reverse transcription polymerase chain reaction (RT-PCR), representing a 15% improvement in sensitivity over the SD Bioline rapid kit. This newly developed FICT could be a useful tool for the rapid diagnosis of RSV infection.


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