scholarly journals The optimum temperature of salicin hydrolysis by enzyme action is independent of the concentrations of substrate and enzyme

The object of the present investigation is to ascertain the influence, if any, on the optimum temperature—temperature of greatest activity—of an enzyme, of the concentration, on the one hand, of the substrate, and, on the other, of the enzyme. The investigation, involving two variables, presents three cases for consideration, according as the concentration of the substrate and the concentration of the enzyme are varied separately or together. An account is given of the results obtained with the enzyme or enzymic function, present in sweet-almond emulsin, which hydrolyses the glucoside salicin with the production of equimolecular quantities of glucose and saligenin. A commercial specimen of Merck’s emulsin was used, while the purity of the salicin employed was ascertained by determining its melting point (200-5°) and its optical activity ([ α ] 21 D = -62·7°). The successive stages in the inquiry may be briefly summarised as follows: (1) a preliminary determination of the activity of the specimen under certain chosen conditions as regards the concentration of the substrate, the temperature, and the duration of the experiment; (2) a preliminary determination of the optimum temperature with the quantity of enzyme found capable of producing 50 per cent, hydrolysis of the substrate under the above conditions; (3) a determination of the activity curves of the enzyme at the temperature thus found, in an action of the same duration for five concentrations of the substrate M/5, M/10, M/15, M/30, and M/50; (4) a determination of the optimum temperature of the enzyme for each of the five concentrations of the substrate in presence of a constant enzyme concentration ; (5) a determination of the optimum temperature of the enzyme for each of the five concentrations of the substrate with quantities of enzyme indicated by the activity curves as capable of producing 70 per cent, hydrolysis of the substrate in the given time: (6) a determination of the optimum temperature of the enzyme for a constant concentration of the substrate in presence of different enzyme concentrations.

1975 ◽  
Vol 68 ◽  
pp. 239-241
Author(s):  
John C. Brown ◽  
H. F. Van Beek

SummaryThe importance and difficulties of determining the height of hard X-ray sources in the solar atmosphere, in order to distinguish source models, have been discussed by Brown and McClymont (1974) and also in this Symposium (Brown, 1975; Datlowe, 1975). Theoretical predictions of this height, h, range between and 105 km above the photosphere for different models (Brown and McClymont, 1974; McClymont and Brown, 1974). Equally diverse values have been inferred from observations of synchronous chromospheric EUV bursts (Kane and Donnelly, 1971) on the one hand and from apparently behind-the-limb events (e.g. Datlowe, 1975) on the other.


Antichthon ◽  
1985 ◽  
Vol 19 ◽  
pp. 57-67 ◽  
Author(s):  
W.K. Lacey
Keyword(s):  
The One ◽  

While the exact meaning of Res Gestae 34, the powers of Augustus at various moments in his principate, and the significance of his auctoritas have been extensively argued, more practical questions about how his political arrangements might have been set into action have not generally excited much interest. In 1974 I put forward a suggestion about how the so-called first settlement of 27 B.C. came about. It was, in brief, that Octavian, as he then was, used the traditional consular mechanisms, and proposed for debate in the Senate a motion, de provinciis consularibus, and this explains why, on the one hand, the result of the debate was that he had provinces allocated to him, and, on the other, claims could be made that the res publica was restored, because one of the things which characterized res publica (as distinct from dictatorship or triumviral rule) was that the determination of who should command which army stationed in the provinces now lay, ostensibly at least, with the publicum consilium, the Senate.


Table II : Quantitative determination of carbonyl compounds at different odour sources (concentrations in ppb) Rendering plant Gelatine plant neighbourhood neighbourhood Formaldehyde 40 16 Acetaldehyde 39 24 Acetone 36 73 Prcpanal 10 -Isobutyraldehyde 10 30 Pentanal 15 19 Hexanal 3.52 Heptanal 12.5 Octanal 10.5 Nonanal 1 2 acids (figure 7). However extractions always involve a serious decrease in sensitivity, while evaporation of the extract produces a solution in 0.1-0.5 ml of solvent, and only 1 pi of it can be brought in the gas chromatograph. Therefore work is in progress to enhance sensitivity by converting acids in­ to halogenated derivatives, which can be GC-analysed with the more sensitive electron-capture detector. For thiols a similar procedure is investigated as with aldehydes. One possibility is absorption of thiols in an alkaline solution and reaction with 2,4-dinitrochlorobenzene, yielding 2,4-dinitrofenylsulfides, which are analysed by HPLC (9). Sane improvements on removal of reagents at the one hand and on separation of sane by-products on the other hand have to be achieved in order to in­ crease the sensitivity with another factor of ten. 5. CONCLUSION The actual scope and limitations of chemical analysis of odour show that all problems can be tackled as far as emission is concerned. For iititiission measurements seme progress is necessary, but there is no essential reason why chemical analysis would be unable to attain the desired sensitivity for all types of odorants. There is no doubt that in a few years the last dif­ ficulties will be solved. In order to achieve real control of odour nui­ sance, automatic measurement is necessary on a long time basis. There again seme technical development is to be expected. Does this mean that machines are going to decide if an odour is pre­ sent or not? By no means, while the population will always be the reference, and psychophysical measurements will be necessary to make chemical analysis possible.


2001 ◽  
Vol 56 (1-2) ◽  
pp. 89-94 ◽  
Author(s):  
Reinhold Deml

Abstract Scolus secretions and hemolymph of caterpillars of Satumia pyri fed with two different foodplants (Crataegus monogyna, Prunus spinosa) were chemically analyzed and their chemical similarities determined. The secondary-compound patterns obtained for the two body fluids showed no significant differences when compared between the two groups of alterna­ tively fed last-instar larvae. Thus, the composition of these fluids of full-grown caterpillars is not influenced by the larval diet. However, younger larvae on P. spinosa revealed a diversity of compounds differing significantly from that of larger caterpillars fed with either C. mono­gyna (both body fluids) or P. spinosa (hemolymph only). This indicates that, on the one hand, the hemolymph composition is adapted to the changing physiological requirements of the given instars whereas, on the other hand, the defensive mixtures remain unaltered in the late larval instars due to a constant spectrum of potential enemies.


2019 ◽  
Vol 150 (4) ◽  
pp. 1853-1870 ◽  
Author(s):  
R. I. Dmytryshyn

AbstractIn the paper the correspondence between a formal multiple power series and a special type of branched continued fractions, the so-called ‘multidimensional regular C-fractions with independent variables’ is analysed providing with an algorithm based upon the classical algorithm and that enables us to compute from the coefficients of the given formal multiple power series, the coefficients of the corresponding multidimensional regular C-fraction with independent variables. A few numerical experiments show, on the one hand, the efficiency of the proposed algorithm and, on the other, the power and feasibility of the method in order to numerically approximate certain multivariable functions from their formal multiple power series.


Author(s):  
D.D. Gabrielyan ◽  
Dan.S. Fedorov ◽  
Den.S. Fedorov

Problem statement. One of the constructing antenna arrays (AA) topic is related to the determination of complex amplitudes at the input of the antenna-feeder path, which, taking into account the distortions introduced by the its, ensure the formation of an amplitude-phase distribution (APD), in which the formed DP differed minimally from the set one. The statement of the problem assumes the known number and coordinates of the location of the emitters, the DP of the radiating element in the composition of the radiating opening, a given radiation pattern. It is required to form an APD in a given opening of the AA, which ensures the formation of a DP that has a minimum deviation from the specified one. To solve the problem, the following algorithm is proposed: determination of the APD at the input of the antenna-feeder path, which ensures the formation of a given DP in the absence of distortions introduced by the antenna-feeder path; measurement for the selected directions of the generated DP with the selected APD in the presence of APD distortions introduced by the antenna-feeder path; formation of a refined APD that ensures the fulfillment of required condition in the presence of distortions introduced by the antenna-feeder path of the AA. Objective. Minimize the root-mean-square deviation of the generated PD from the one specified for the AP with the opening of an arbitrary geometry. Results. The results obtained showed: The formulation of the problem of synthesis of the APD allows us to consider, within the framework of a single approach, AA with different geometries, including AA with a non-planar radiating opening, no restrictions are imposed on the shape of the boundary, and linear (quasi-ring) AA, the emitters of which are located along an arbitrary mane. Using the proposed algorithm for the synthesis of APD, which includes three main stages: the formation of APD for a given DP; measurement of complex values of the generated DP; refinement of the APD by determining the corrections while minimizing the standard deviation (SD) between the specified and formed at the first stage of the DP, allows us to form an APD that provides a minimum SD between the specified and formed DP. The performed studies have shown that when choosing the number of angular directions in each plane, comparable to the number of emitters in this plane, there is a large difference between the given and formed DP. When the number of angular directions for measuring the DP is approximately three times greater than the number of emitters in a given plane, the SD between the specified and formed DP is close to the minimum value and practically does not change with further increase.


Author(s):  
Gleb L. Kotkin ◽  
Valeriy G. Serbo

If the potential energy is independent of time, the energy of the system remains constant during the motion of a closed system. A system with one degree of freedom allows for the determination of the law of motion in quadrature. In this chapter, the authors consider motion of the particles in the one-dimensional fields. They discuss also how the law and the period of a particle moving in the potential field change due to adding to the given field a small correction.


1831 ◽  
Vol 121 ◽  
pp. 417-422 ◽  

That several of the planets as well as that which we inhabit are surrounded by atmosphere, astronomical observations have long since established; the extent, however, to which in particular planets such atmospheres are diffused, is as yet not satisfactorily determined. The former rests principally upon phenomena observed on the planets’ discs, whilst the latter derives its support chiefly from those detected at or near their respective limbs. Every night, nay almost every hour, may give us indication of the one, whilst years are sometimes necessary, as in the case of planets unattended by satellites, to help us to the other; thus the hypothesis of the extensive atmosphere of Mars derives its origin from the observations of Cassini and Roëmer, and has stood more than a century and a half without refutation or support. The observations to which I allude formed part of a series undertaken for the determination of the parallax of Mars, and are recorded in the Mémoires de l’Académie des Sciences. Cassini’s were made at Briare and at La Charité sur Loire; whilst Roémer’s was obtained at the Royal Observatory of Paris.


Author(s):  
Marion Ledwig

Spohn's decision model, an advancement of Fishburn's theory, is valuable for making explicit the principle used also by other thinkers that 'any adequate quantitative decision model must not explicitly or implicitly contain any subjective probabilities for acts.' This principle is not used in the decision theories of Jeffrey or of Luce and Krantz. According to Spohn, this principle is important because it has effects on the term of action, on Newcomb's problem, and on the theory of causality and the freedom of the will. On the one hand, I will argue against Spohn with Jeffrey that the principle has to be given up. On the other, I will try to argue against Jeffrey that the decision-maker ascribes subjective probabilities to actions on the condition of the given decision situation.


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