Synaptic structure and its alteration with environmental temperature: a study by light and electron microscopy of the central nervous system of lizards

1961 ◽  
Vol 154 (955) ◽  
pp. 151-172 ◽  
Keyword(s):  
Electron Microscopy ◽  
Central Nervous System ◽  
Nervous System ◽  
Environmental Temperature ◽  
Light And Electron Microscopy ◽  
Synaptic Structure ◽  
The Central Nervous System

Localization of Actin Filaments in the Central Nervous System Using Phalloidin and Correlative Light and Electron Microscopy

1999 ◽  
Vol 5 (S2) ◽  
pp. 498-499
Author(s):  
Francisco Capani ◽  
Maryann E. Martone ◽  
Thomas J. Deerinck ◽  
Mark H. Ellisman
Keyword(s):  
Electron Microscopy ◽  
Central Nervous System ◽  
Nervous System ◽  
Actin Filaments ◽  
Cold Water ◽  
Light And Electron Microscopy ◽  
Actin Binding ◽  
Electron Microscopic Level ◽  
The Central Nervous System ◽  
Structural Methods

Determination of the fine organization of actin networks is important for understanding different functional aspects of the central nervous system (CNS) such as plastic events. Early studies investigating the localization of actin filaments at the ultrastructural level relied on structural methods or antibodies raised against actin isoforms. More recently, many investigators have employed the actin-binding peptide phalloidin conjugated to various fluorescent molecules. By conjugating phalloidin to the fluorophore eosin, we have been able to localize f-actin at the electron microscopic level using photooxidation of diaminobenzidine (DAB) by eosin. The goal of this study is to provide a better description of the cellular and subcellular localization of actin filaments at the light and electron microscopy level based on the high binding affinity of phalloidin for actin and utilizing this novel method.Tissues were obtained from male Sprague Dawley rats that were perfused transcardially under deep anesthesia with normal rat Ringer's solution at 35°C followed by fixative. The fixative contained 4 % paraformaldehyde and different concentrations of glutaraldehyde, ranging from 0.1- 2.5%. Sections of cerebellum, striatum and hippocampus which were cut at a thickness of 50-80 μm with a Vibratome were incubated with phalloidin-eosin in a solution of 0.05% in 0.5% cold water fish gelatin/50mM glycine-PBS (“working buffer”) for 2 hrs. As a control, phalloidin-eosin was omitted for one set of tissues.

Distribution of adrenomedullin-like immunoreactivity in the rat central nervous system by light and electron microscopy

2000 ◽  
Vol 853 (2) ◽  
pp. 245-268 ◽  
Author(s):  
Julia Serrano ◽  
L.Otto Uttenthal ◽  
Alfredo Martı́nez ◽  
A.Patricia Fernández ◽  
Javier Martı́nez de Velasco ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Central Nervous System ◽  
Nervous System ◽  
Light And Electron Microscopy

scholarly journals Central nervous system virion detection in acute measles: histopathological, ultrastructural and pathogenetic aspects

1995 ◽  
Vol 37 (2) ◽  
pp. 137-143
Author(s):  
C.L.P. Lancellotti ◽  
C.E.P. Corbett ◽  
M.I.S. Duarte
Keyword(s):  
Electron Microscopy ◽  
Central Nervous System ◽  
Nervous System ◽  
Clinical Diagnosis ◽  
Clinical Manifestations ◽  
Histopathological Changes ◽  
Ultrastructural Studies ◽  
The Central Nervous System ◽  
Neurological Alterations

Histopathological and ultrastructural studies of 23 patients who died with clinical diagnosis of measles were carried out. In 12 cases viral nucleocapsids were searched by electron microscopy and detected in 100% of the cases in the lungs and in 50% of the cases in the central nervous system. They were mostly intranuclear. Histopathological changes associated to neurological alterations and the detection of virion are discussed in relation to acute and delayed clinical manifestations.

scholarly journals Development of the central nervous system in guinea pig (Cavia porcellus, Rodentia, Caviidae)

2016 ◽  
Vol 36 (8) ◽  
pp. 753-760 ◽  
Author(s):  
Fernanda Menezes de Oliveira e Silva ◽  
Dayane Alcantara ◽  
Rafael Cardoso Carvalho ◽  
Phelipe Oliveira Favaron ◽  
Amilton Cesar dos Santos ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Central Nervous System ◽  
Nervous System ◽  
Guinea Pig ◽  
Brain Tissue ◽  
Light And Electron Microscopy ◽  
Cauda Equina ◽  
Neural Tube Closure ◽  
Morphological Studies ◽  
The Central Nervous System

Abstract: This study describes the development of the central nervous system in guinea pigs from 12th day post conception (dpc) until birth. Totally, 41 embryos and fetuses were analyzed macroscopically and by means of light and electron microscopy. The neural tube closure was observed at day 14 and the development of the spinal cord and differentiation of the primitive central nervous system vesicles was on 20th dpc. Histologically, undifferentiated brain tissue was observed as a mass of mesenchymal tissue between 18th and 20th dpc, and at 25th dpc the tissue within the medullary canal had higher density. On day 30 the brain tissue was differentiated on day 30 and the spinal cord filling throughout the spinal canal, period from which it was possible to observe cerebral and cerebellar stratums. At day 45 intumescences were visualized and cerebral hemispheres were divided, with a clear division between white and gray matter in brain and cerebellum. Median sulcus of the dorsal spinal cord and the cauda equina were only evident on day 50. There were no significant structural differences in fetuses of 50 and 60 dpc, and animals at term were all lissencephalic. In conclusion, morphological studies of the nervous system in guinea pig can provide important information for clinical studies in humans, due to its high degree of neurological maturity in relation to its short gestation period, what can provide a good tool for neurological studies.

Monoamine synaptic structure and localization in the central nervous system

1990 ◽  
Vol 15 (1) ◽  
pp. 20-33 ◽  
Author(s):  
Bruce E. Maley ◽  
Mary Gail Engle ◽  
Suzanne Humphreys ◽  
Debra A. Vascik ◽  
Kimberly A. Howes ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Synaptic Structure ◽  
The Central Nervous System

scholarly journals Cellular and molecular mechanisms of the demyelination in the central nervous system and cell therapy approaches

2017 ◽  
Vol 5 (1) ◽  
pp. 74-78
Author(s):  
V. Tsymbaliuk ◽  
V. Semenova ◽  
L. Pichkur ◽  
O. Velychko ◽  
D. Egorova
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Cell Therapy ◽  
Molecular Mechanisms ◽  
Molecular Genetic ◽  
Light And Electron Microscopy ◽  
Cellular Level ◽  
Molecular Features ◽  
The Central Nervous System ◽  
Demyelinating Disorders

The review summarizes the current concepts of cell-tissue and molecular features of development of demyelinating processes in the central nervous system related to multiple sclerosis and its animal model – allergic encephalomyelitis. An analysis of recently published studies of this pathology, carried out with light and electron microscopy and immunohistochemical and molecular genetic methods, is given. New methodological approaches to the study of the pathomorhological aspects of demyelinating disorders allowed receiving in-depth understanding of the etiology and mechanisms of demyelination processes in the brain and spinal cord tissues at the cellular level and identifying the ways to develop effective modern methods of pathogenetic treatment of these diseases using cell therapy.

scholarly journals Cerebrospinal Lipodystrophy in Swine

1968 ◽  
Vol 5 (1) ◽  
pp. 67-74 ◽  
Author(s):  
W. Kay Read ◽  
Charles H. Bridges
Keyword(s):  
Electron Microscopy ◽  
Central Nervous System ◽  
Nervous System ◽  
Neuronal Inclusions ◽  
The Central Nervous System

Spontaneous lipidosis of the central nervous system of two Yorkshire swine is reported. The presence of membranous cytoplasmic neuronal inclusions similar to those reported in Tay-Sach's disease in man was confirmed by electron microscopy.

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